(Circular) (Six-base) MAPSORT of: pFN21AB7727.seq Check: 3149 from: 1 to: 5998 pFN21AB7727 5998 bp With 182 enzymes: SgfI * March 28, 2012 12:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2629 3003 3341 1937 Size: 692 374 338 4594 Fragments arranged by size: 4594 692 374 338 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2903 3213 3222 3250 4061 5445 5713 1632 Size: 1271 310 9 28 811 1384 268 1917 Fragments arranged by size: 1917 1384 1271 811 310 268 28 9 AfeI AGC'GCT Cuts at: 2260 2260 Size: 5998 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 2210 4829 2210 Size: 2619 3379 AhdI GACnn_n'nnGTC Cuts at: 3247 4727 3247 Size: 1480 4518 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5998 AlwNI CAG_nnn'CTG Cuts at: 2815 2926 3741 5313 2815 Size: 111 815 1572 3500 Fragments arranged by size: 3500 1572 815 111 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 3307 3533 3685 5794 1493 Size: 1814 226 152 2109 1697 Fragments arranged by size: 2109 1814 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 2164 3323 1870 Size: 73 221 1159 4545 Fragments arranged by size: 4545 1159 221 73 BamHI G'GATC_C Cuts at: 3328 3695 3328 Size: 367 5631 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 3319 4774 618 Size: 325 217 627 381 1151 1455 1842 Fragments arranged by size: 1842 1455 1151 627 381 325 217 BanII G_rGCy'C Cuts at: 1391 2316 3317 1391 Size: 925 1001 4072 Fragments arranged by size: 4072 1001 925 BbeI G_GCGC'C Cuts at: 2172 2172 Size: 5998 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5027 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2826 2860 4189 4223 1903 Size: 34 889 34 1329 34 3678 Fragments arranged by size: 3678 1329 889 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2159 4609 136 Size: 107 122 71 631 605 487 2450 1525 Fragments arranged by size: 2450 1525 631 605 487 122 107 71 BglII A'GATC_T Cuts at: 5993 5993 Size: 5998 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 2501 4059 5215 1790 Size: 711 1558 1156 2573 Fragments arranged by size: 2573 1558 1156 711 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3729 4687 442 Size: 416 53 83 399 24 2312 958 1753 Fragments arranged by size: 2312 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2330 2837 3007 4658 1239 Size: 1091 507 170 1651 2579 Fragments arranged by size: 2579 1651 1091 507 170 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2357 2886 4123 4988 5286 2357 Size: 529 1237 865 298 3069 Fragments arranged by size: 3069 1237 865 529 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4661 882 Size: 617 3162 2219 Fragments arranged by size: 3162 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3789 493 Size: 711 2585 2702 Fragments arranged by size: 2702 2585 711 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 3805 4187 4822 Size: 53 83 186 936 636 1636 382 635 Cuts at: 4822 275 Size: 1451 Fragments arranged by size: 1636 1451 936 636 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2210 3102 4431 4829 5103 5250 5661 2210 Size: 892 1329 398 274 147 411 2547 Fragments arranged by size: 2547 1329 892 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2353 2383 1178 Size: 30 165 30 950 30 4793 Fragments arranged by size: 4793 950 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2612 1186 Size: 1426 4572 BseYI C'CCAG_C Cuts at: 2268 2880 5201 5697 2268 Size: 612 2321 496 2569 Fragments arranged by size: 2569 2321 612 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 3139 1163 Size: 573 606 797 4022 Fragments arranged by size: 4022 797 606 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2236 4059 4144 5215 2236 Size: 1823 85 1071 3019 Fragments arranged by size: 3019 1823 1071 85 BsmI GAATG_Cn' Cuts at: 2348 3509 3602 2348 Size: 1161 93 4744 Fragments arranged by size: 4744 1161 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2236 2316 2501 3317 4059 4144 5215 Size: 399 446 80 185 816 742 85 1071 Cuts at: 5215 1391 Size: 2174 Fragments arranged by size: 2174 1071 816 742 446 399 185 85 80 BspEI T'CCGG_A Cuts at: 3102 5661 3102 Size: 2559 3439 BspHI T'CATG_A Cuts at: 3887 5617 3887 Size: 1730 4268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2934 3353 844 Size: 2090 419 3489 Fragments arranged by size: 3489 2090 419 BsrBI CCG'CTC Cuts at: 1394 1823 2887 3885 1394 Size: 429 1064 998 3507 Fragments arranged by size: 3507 1064 998 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2450 2802 4493 4667 65 Size: 1183 1202 352 1691 174 1396 Fragments arranged by size: 1691 1396 1202 1183 352 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 2210 4642 4829 1700 Size: 110 297 103 2432 187 2869 Fragments arranged by size: 2869 2432 297 187 110 103 BsrGI T'GTAC_A Cuts at: 96 2491 96 Size: 2395 3603 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 4058 5070 4058 Size: 1012 4986 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5998 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 2667 3216 1184 Size: 1483 549 3966 Fragments arranged by size: 3966 1483 549 BstXI CCAn_nnnn'nTGG Cuts at: 2468 2797 2468 Size: 329 5669 BstYI r'GATC_y Cuts at: 1972 2395 3099 3328 3695 4080 4097 5549 5635 Size: 423 704 229 367 385 17 1452 86 Cuts at: 5635 5647 5993 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 704 423 385 367 346 229 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3109 3109 Size: 5998 BtgI C'CryG_G Cuts at: 513 1065 2018 2202 513 Size: 552 953 184 4309 Fragments arranged by size: 4309 953 552 184 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2833 487 Size: 683 56 1607 3652 Fragments arranged by size: 3652 1607 683 56 BtsI GCAGTG_nn' Cuts at: 3514 4308 4328 3514 Size: 794 20 5184 Fragments arranged by size: 5184 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2799 3445 4334 Size: 54 1425 50 275 18 969 646 889 Cuts at: 4334 5963 8 Size: 1629 43 Fragments arranged by size: 1629 1425 969 889 646 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2991 3928 1353 Size: 1638 937 3423 Fragments arranged by size: 3423 1638 937 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4585 4959 5105 387 Size: 1064 138 2996 374 146 1280 Fragments arranged by size: 2996 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2837 2903 3007 3213 3222 3250 Size: 393 698 507 66 104 206 9 28 Cuts at: 3250 4061 4658 5445 5713 1239 Size: 811 597 787 268 1524 Fragments arranged by size: 1524 811 787 698 597 507 393 268 206 104 66 28 9 EcoNI CCTnn'n_nnAGG Cuts at: 2585 2585 Size: 5998 EcoRI G'AATT_C Cuts at: 3307 3685 3307 Size: 378 5620 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2351 2351 Size: 5998 FspI TGC'GCA Cuts at: 4504 4504 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 2172 2262 3045 5145 1678 Size: 494 90 783 2100 2531 Fragments arranged by size: 2531 2100 783 494 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3056 3088 3233 3265 4639 Size: 32 135 32 1484 32 145 32 1374 Cuts at: 4639 4671 4713 4745 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1484 1374 145 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2749 3342 3588 669 Size: 988 281 811 593 246 3079 Fragments arranged by size: 3079 988 811 593 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KasI G'GCGC_C Cuts at: 2168 2168 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5112 5296 1305 Size: 3807 184 2007 Fragments arranged by size: 3807 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2801 10 Size: 54 1425 343 969 3207 Fragments arranged by size: 3207 1425 969 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2515 2694 3956 4315 4474 518 Size: 648 1349 179 1262 359 159 2042 Fragments arranged by size: 2042 1349 1262 648 359 179 159 MspA1I CmG'CkG Cuts at: 1606 2020 3414 4091 5239 5484 1606 Size: 414 1394 677 1148 245 2120 Fragments arranged by size: 2120 1394 1148 677 414 245 NaeI GCC'GGC Cuts at: 2109 2109 Size: 5998 NarI GG'CG_CC Cuts at: 2169 2169 Size: 5998 NcoI C'CATG_G Cuts at: 513 1065 2202 513 Size: 552 1137 4309 Fragments arranged by size: 4309 1137 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NgoMIV G'CCGG_C Cuts at: 2107 2107 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 3158 3356 4901 1132 Size: 97 1929 198 1545 2229 Fragments arranged by size: 2229 1929 1545 198 97 PasI CC'CwG_GG Cuts at: 1348 2081 1348 Size: 733 5265 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 2590 2664 1108 Size: 1482 74 4442 Fragments arranged by size: 4442 1482 74 PfoI T'CCnGG_A Cuts at: 2541 2541 Size: 5998 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2167 2199 4067 4099 5580 5612 2167 Size: 32 1868 32 1481 32 2553 Fragments arranged by size: 2553 1868 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3029 3061 3029 Size: 32 5966 PstI C_TGCA'G Cuts at: 830 2046 3350 830 Size: 1216 1304 3478 Fragments arranged by size: 3478 1304 1216 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 SacII CC_GC'GG Cuts at: 2021 2021 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SexAI A'CCwGG_T Cuts at: 2793 2793 Size: 5998 SfcI C'TryA_G Cuts at: 826 1045 2042 2227 3346 4481 5162 5353 826 Size: 219 997 185 1119 1135 681 191 1471 Fragments arranged by size: 1471 1135 1119 997 681 219 191 185 SfiI GGCCn_nnn'nGGCC Cuts at: 2159 2159 Size: 5998 SfoI GGC'GCC Cuts at: 2170 2170 Size: 5998 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 3325 1872 Size: 1453 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2336 2901 4102 5003 5265 820 Size: 197 926 393 565 1201 901 262 1553 Fragments arranged by size: 1553 1201 926 901 565 393 262 197 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 2761 3922 5 Size: 47 2709 1161 2081 Fragments arranged by size: 2709 2081 1161 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5998 StyI C'CwwG_G Cuts at: 513 1065 2202 2941 3437 513 Size: 552 1137 739 496 3074 Fragments arranged by size: 3074 1137 739 552 496 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2289 2439 2954 4025 4042 4195 4380 Size: 1255 709 150 515 1071 17 153 185 Cuts at: 4380 4825 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1071 709 515 445 185 153 150 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2491 4244 5956 Size: 274 80 33 51 1443 514 1753 1712 Cuts at: 5956 96 Size: 138 Fragments arranged by size: 1753 1712 1443 514 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2353 2385 3002 3034 5958 5990 2353 Size: 32 617 32 2924 32 2361 Fragments arranged by size: 2924 2361 617 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 2677 3334 2677 Size: 657 5341 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3090 3090 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 3323 1870 Size: 1453 4545 XmnI GAAnn'nnTTC Cuts at: 4127 4127 Size: 5998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SanDI SapI SrfI SwaI