################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI Fnu4HI FokI FseI FspI HaeII HapII HhaI Hin1I HinP1I HindIII HpaI HpaII HphI ItaI KasI KpnI KspI MaeII MaeIII MfeI MluI MluNI MroI MscI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TseI Tsp45I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AlwI 5 GATCC GGATC(4/5) AlwNI 448 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 163 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 341 GAAGAC GAAGAC(2/6) BclI 138 TGATCA T^GATCA BpmI 188 CTCCAG CTGGAG(16/14) BpuAI 341 GAAGAC GAAGAC(2/6) BsaJI 87 CCAAGG C^C[ACGT][ACGT]GG BsmAI 202 GAGAC GTCTC(1/5) BsmI 113 GAATGC GAATGC(1/-1) BssKI 13 CCTGG ^CC[ACGT]GG BstNI 13 CCTGG CC^[AT]GG BstYI 4 AGATCC [GA]^GATC[CT] Cac8I 183 GCCAGC GC[ACGT]^[ACGT]GC EarI 309 GAAGAG CTCTTC(1/4) EcoRI 403 GAATTC G^AATTC EcoRII 13 CCTGG ^CC[AT]GG EcoT14I 87 CCAAGG C^C[AT][AT]GG FbaI 138 TGATCA T^GATCA HaeIII 243 GGCC GG^CC HgaI 117 GCGTC GACGC(5/10) HincII 119 GTCAAC GT[CT]^[GA]AC HindII 119 GTCAAC GT[CT]^[GA]AC Ksp632I 309 GAAGAG CTCTTC(1/4) MflI 4 AGATCC [GA]^GATC[CT] MseI 299 TTAA T^TAA MslI 469 CACGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 142 CAGCTG C[AC]G^C[GT]G MvaI 13 CCTGG CC^[AT]GG NlaIV 168 GGTCCC GG[ACGT]^[ACGT]CC NspI 361 ACATGC [GA]CATG^[CT] PflFI 163 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 63 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 142 CAGCTG CAG^CTG ScrFI 13 CCTGG CC^[ACGT]GG SfaNI 450 GCATC GCATC(5/9) StyI 87 CCAAGG C^C[AT][AT]GG Tru9I 299 TTAA T^TAA TspRI 65 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 163 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 460 TCTAGA T^CTAGA XhoII 4 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AcsI 175 GAATTT [GA]^AATT[CT] 403 GAATTC ApoI 175 GAATTT [GA]^AATT[CT] 403 GAATTC AvaII 69 GGTCC G^G[AT]CC 168 GGTCC BfaI 409 CTAG C^TAG 461 CTAG BsmFI 61 GGGAC GGGAC(10/14) 169 GTCCC BsrI 66 ACTGG ACTGG(1/-1) 8 CCAGT MaeI 409 CTAG C^TAG 461 CTAG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BsaBI Cfr13I DpnII HinfI MamI MboI MboII MnlI NdeII NlaIII Sau3AI Sau96I TfiI Tsp509I