################################## # NO CUT ENZYMES ( 193 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeIII HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MboII MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 25 GTAGAC GT^[AC][GT]AC AlwI 380 GGATC GGATC(4/5) Aor51HI 160 AGCGCT AGC^GCT AvaII 242 GGACC G^G[AT]CC BbvI 94 GCAGC GCAGC(8/12) BpmI 246 CTGGAG CTGGAG(16/14) BsaWI 29 ACCGGA [AT]^CCGG[AT] BsmFI 241 GGGAC GGGAC(10/14) BspHI 99 TCATGA T^CATGA BssKI 245 CCTGG ^CC[ACGT]GG BstBI 167 TTCGAA TT^CGAA BstNI 245 CCTGG CC^[AT]GG BstYI 380 GGATCT [GA]^GATC[CT] Cac8I 90 GCTTGC GC[ACGT]^[ACGT]GC CfoI 161 GCGC GCG^C Cfr13I 242 GGACC G^G[ACGT]CC DdeI 235 CTCAG C^T[ACGT]AG DpnII 381 GATC ^GATC DraI 385 TTTAAA TTT^AAA DraII 241 GGGACCT [GA]G^G[ACGT]CC[CT] Eco47III 160 AGCGCT AGC^GCT EcoO109I 241 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 245 CCTGG ^CC[AT]GG Fnu4HI 94 GCAGC GC^[ACGT]GC HaeII 160 AGCGCT [GA]GCGC^[CT] HapII 30 CCGG C^CGG HhaI 161 GCGC GCG^C HinP1I 161 GCGC G^CGC HindIII 88 AAGCTT A^AGCTT HinfI 154 GAATC G^A[ACGT]TC HpaII 30 CCGG C^CGG ItaI 94 GCAGC GC^[ACGT]GC MaeIII 199 GTCAC ^GT[ACGT]AC MboI 381 GATC ^GATC MflI 380 GGATCT [GA]^GATC[CT] MnlI 256 GAGG CCTC(7/6) MspI 30 CCGG C^CGG MvaI 245 CCTGG CC^[AT]GG NdeII 381 GATC ^GATC NspV 167 TTCGAA TT^CGAA PpuMI 241 GGGACCT [GA]G^G[AT]CC[CT] RcaI 99 TCATGA T^CATGA Sau3AI 381 GATC ^GATC Sau96I 242 GGACC G^G[ACGT]CC ScrFI 245 CCTGG CC^[ACGT]GG SfuI 167 TTCGAA TT^CGAA SmlI 253 CTTGAG C^T[CT][GA]AG SpeI 339 ACTAGT A^CTAGT TaqI 168 TCGA T^CGA TfiI 154 GAATC G^A[AT]TC TseI 94 GCAGC G^C[AT]GC Tsp45I 199 GTCAC ^GT[GC]AC TthHB8I 168 TCGA T^CGA XhoII 380 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AcsI 139 AAATTC [GA]^AATT[CT] 277 AAATTC ApoI 139 AAATTC [GA]^AATT[CT] 277 AAATTC BfaI 217 CTAG C^TAG 340 CTAG HphI 331 GGTGA GGTGA(8/7) 200 TCACC MaeI 217 CTAG C^TAG 340 CTAG NlaIII 54 CATG CATG^ 100 CATG NlaIV 241 GGGACC GG[ACGT]^[ACGT]CC 248 GGAGCC ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### AluI MseI Tru9I Tsp509I