(Circular) (Six-base) MAPSORT of: pFN21AE6581.seq Check: 7324 from: 1 to: 5101 pFN21AE6581 5101 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2911 278 Size: 53 83 186 936 1375 2468 Fragments arranged by size: 2468 1375 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2031 2444 1937 Size: 94 413 4594 Fragments arranged by size: 4594 413 94 Acc65I G'GTAC_C Cuts at: 2422 2422 Size: 5101 AclI AA'CG_TT Cuts at: 1563 3228 3601 1563 Size: 1665 373 3063 Fragments arranged by size: 3063 1665 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3164 4548 4816 1632 Size: 1532 1384 268 1917 Fragments arranged by size: 1917 1532 1384 268 AfeI AGC'GCT Cuts at: 2167 2167 Size: 5101 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4904 AflIII A'CryG_T Cuts at: 1203 4000 4977 1203 Size: 2797 977 1327 Fragments arranged by size: 2797 1327 977 AgeI A'CCGG_T Cuts at: 3932 3932 Size: 5101 AhdI GACnn_n'nnGTC Cuts at: 3830 3830 Size: 5101 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5101 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5101 AlwNI CAG_nnn'CTG Cuts at: 2844 4416 2844 Size: 1572 3529 ApaLI G'TGCA_C Cuts at: 3158 4314 3158 Size: 1156 3945 ApoI r'AATT_y Cuts at: 1493 2144 2282 2410 2636 2788 4897 1493 Size: 651 138 128 226 152 2109 1697 Fragments arranged by size: 2109 1697 651 226 152 138 128 AseI AT'TA_AT Cuts at: 160 1032 3655 160 Size: 872 2623 1606 Fragments arranged by size: 2623 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5101 AvaI C'yCGr_G Cuts at: 1870 1943 2426 1870 Size: 73 483 4545 Fragments arranged by size: 4545 483 73 BamHI G'GATC_C Cuts at: 2431 2798 2431 Size: 367 4734 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2422 3877 618 Size: 325 217 627 635 1455 1842 Fragments arranged by size: 1842 1455 635 627 325 217 BanII G_rGCy'C Cuts at: 1391 2420 1391 Size: 1029 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3292 3326 1903 Size: 34 1355 34 3678 Fragments arranged by size: 3678 1355 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2993 4203 2993 Size: 1210 3891 BclI T'GATC_A Cuts at: 1548 1548 Size: 5101 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3712 136 Size: 107 122 71 631 605 2040 1525 Fragments arranged by size: 2040 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5096 5096 Size: 5101 BlpI GC'TnA_GC Cuts at: 2518 2518 Size: 5101 Bme1580I G_kGCm'C Cuts at: 1790 3162 4318 1790 Size: 1372 1156 2573 Fragments arranged by size: 2573 1372 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5101 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2832 3790 442 Size: 416 53 83 399 24 1415 958 1753 Fragments arranged by size: 1753 1415 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5101 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2272 3761 1239 Size: 1033 1489 2579 Fragments arranged by size: 2579 1489 1033 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2279 3226 4091 4389 2279 Size: 947 865 298 2991 Fragments arranged by size: 2991 947 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3764 882 Size: 617 2265 2219 Fragments arranged by size: 2265 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2892 493 Size: 711 1688 2702 Fragments arranged by size: 2702 1688 711 BsaBI GATnn'nnATC Cuts at: 5095 5095 Size: 5101 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2908 3290 3925 275 Size: 53 83 186 936 1375 382 635 1451 Fragments arranged by size: 1451 1375 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2034 3534 3932 4206 4353 4764 2034 Size: 1500 398 274 147 411 2371 Fragments arranged by size: 2371 1500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4876 Fragments arranged by size: 4876 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5101 BseYI C'CCAG_C Cuts at: 4304 4800 4304 Size: 496 4605 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4528 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2551 3312 4340 1540 Size: 275 186 550 761 1028 2301 Fragments arranged by size: 2301 1028 761 550 275 186 BsiHKAI G_wGCw'C Cuts at: 3162 3247 4318 3162 Size: 85 1071 3945 Fragments arranged by size: 3945 1071 85 BsmI GAATG_Cn' Cuts at: 2612 2705 2612 Size: 93 5008 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2420 3162 3247 4318 1391 Size: 399 630 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 630 399 85 BspEI T'CCGG_A Cuts at: 4764 4764 Size: 5101 BspHI T'CATG_A Cuts at: 2104 2990 4720 2104 Size: 886 1730 2485 Fragments arranged by size: 2485 1730 886 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2456 844 Size: 1612 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2988 1394 Size: 429 1165 3507 Fragments arranged by size: 3507 1165 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3596 3770 65 Size: 1183 2348 174 1396 Fragments arranged by size: 2348 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3745 3932 1700 Size: 110 1935 187 2869 Fragments arranged by size: 2869 1935 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5101 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5101 BssSI C'ACGA_G Cuts at: 3161 4173 3161 Size: 1012 4089 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5101 BstBI TT'CG_AA Cuts at: 2173 3905 2173 Size: 1732 3369 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5101 BstYI r'GATC_y Cuts at: 1972 2385 2431 2798 3183 3200 4652 4738 4750 Size: 413 46 367 385 17 1452 86 12 Cuts at: 4750 5096 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 413 385 367 346 86 46 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3610 Fragments arranged by size: 3610 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4362 Fragments arranged by size: 4362 683 56 BtsI GCAGTG_nn' Cuts at: 2617 3411 3431 2617 Size: 794 20 4287 Fragments arranged by size: 4287 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5101 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5066 DraI TTT'AAA Cuts at: 2392 2405 2392 Size: 13 5088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4108 809 Size: 507 2792 1802 Fragments arranged by size: 2792 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2548 3437 5066 Size: 54 1425 50 275 18 718 889 1629 Cuts at: 5066 8 Size: 43 Fragments arranged by size: 1629 1425 889 718 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2548 1537 Size: 275 736 4090 Fragments arranged by size: 4090 736 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3031 1353 Size: 1678 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3688 4062 4208 387 Size: 1064 138 2099 374 146 1280 Fragments arranged by size: 2099 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 3164 3761 4548 4816 1239 Size: 393 640 892 597 787 268 1524 Fragments arranged by size: 1524 892 787 640 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2247 2247 Size: 5101 EcoRI G'AATT_C Cuts at: 2410 2788 2410 Size: 378 4723 FspI TGC'GCA Cuts at: 3607 3607 Size: 5101 HaeII r_GCGC'y Cuts at: 1678 2169 4248 1678 Size: 491 2079 2531 Fragments arranged by size: 2531 2079 491 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3742 3774 3816 3848 1373 Size: 32 135 32 2170 32 42 32 2626 Fragments arranged by size: 2626 2170 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2445 2691 669 Size: 988 281 507 246 3079 Fragments arranged by size: 3079 988 507 281 246 HindIII A'AGCT_T Cuts at: 748 2093 748 Size: 1345 3756 HpaI GTT'AAC Cuts at: 2691 2691 Size: 5101 KpnI G_GTAC'C Cuts at: 2426 2426 Size: 5101 MfeI C'AATT_G Cuts at: 2700 2700 Size: 5101 MluI A'CGCG_T Cuts at: 4977 4977 Size: 5101 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4215 4399 1305 Size: 2910 184 2007 Fragments arranged by size: 2910 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3279 Fragments arranged by size: 3279 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3059 3418 3577 518 Size: 648 1893 359 159 2042 Fragments arranged by size: 2042 1893 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2517 3194 4342 4587 1606 Size: 911 677 1148 245 2120 Fragments arranged by size: 2120 1148 911 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3610 Fragments arranged by size: 3610 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5101 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5101 NotI GC'GGCC_GC Cuts at: 2548 2548 Size: 5101 NspI r_CATG'y Cuts at: 1132 1229 2459 4004 1132 Size: 97 1230 1545 2229 Fragments arranged by size: 2229 1545 1230 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5101 PciI A'CATG_T Cuts at: 4000 4000 Size: 5101 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5101 PmeI GTTT'AAAC Cuts at: 2405 2405 Size: 5101 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3170 3202 4683 4715 3170 Size: 32 1481 32 3556 Fragments arranged by size: 3556 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2247 2247 Size: 5101 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5101 PsiI TTA'TAA Cuts at: 2671 2671 Size: 5101 PstI C_TGCA'G Cuts at: 830 2453 830 Size: 1623 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5101 SalI G'TCGA_C Cuts at: 1936 2443 1936 Size: 507 4594 SbfI CC_TGCA'GG Cuts at: 2453 2453 Size: 5101 ScaI AGT'ACT Cuts at: 3349 5061 3349 Size: 1712 3389 SfcI C'TryA_G Cuts at: 826 1045 2449 3584 4265 4456 826 Size: 219 1404 1135 681 191 1471 Fragments arranged by size: 1471 1404 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5101 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5101 SmaI CCC'GGG Cuts at: 1872 2428 1872 Size: 556 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 3205 4106 4368 820 Size: 197 926 315 947 901 262 1553 Fragments arranged by size: 1553 947 926 901 315 262 197 SnaBI TAC'GTA Cuts at: 493 2892 493 Size: 2399 2702 SpeI A'CTAG_T Cuts at: 152 2344 152 Size: 2192 2909 SphI G_CATG'C Cuts at: 1132 2459 1132 Size: 1327 3774 SspI AAT'ATT Cuts at: 5 52 3025 5 Size: 47 2973 2081 Fragments arranged by size: 2973 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5101 StyI C'CwwG_G Cuts at: 513 1065 2004 2540 513 Size: 552 939 536 3074 Fragments arranged by size: 3074 939 552 536 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 3128 3145 3298 3483 3928 325 Size: 1255 480 1068 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1068 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3347 5059 96 Size: 274 80 33 51 1443 1370 1712 138 Fragments arranged by size: 1712 1443 1370 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5061 5093 5061 Size: 32 5069 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5101 XbaI T'CTAG_A Cuts at: 2437 2437 Size: 5101 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5101 XmaI C'CCGG_G Cuts at: 1870 2426 1870 Size: 556 4545 XmnI GAAnn'nnTTC Cuts at: 3230 3230 Size: 5101 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2909 276 Size: 53 83 186 936 1375 2468 Fragments arranged by size: 2468 1375 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI