################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I CfoI Cfr10I CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MflI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 869 GGTACC G^GTACC AcsI 211 AAATTT [GA]^AATT[CT] ApoI 211 AAATTT [GA]^AATT[CT] Asp718I 869 GGTACC G^GTACC AspHI 563 GTGCTC G[AT]GC[AT]^C AvaI 929 CTCGAG C^[CT]CG[GA]G AvaII 309 GGTCC G^G[AT]CC BalI 881 TGGCCA TGG^CCA BbsI 1075 GAAGAC GAAGAC(2/6) BfaI 220 CTAG C^TAG BlpI 324 GCTTAGC GC^T[ACGT]AGC BmyI 563 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 324 GCTTAGC GC^T[ACGT]AGC BpuAI 1075 GAAGAC GAAGAC(2/6) BsaI 903 GGTCTC GGTCTC(1/5) BsaJI 317 CCAAGG C^C[ACGT][ACGT]GG BsiHKAI 563 GTGCTC G[AT]GC[AT]^C BsoBI 929 CTCGAG C^[CT]CG[GA]G Bsp1286I 563 GTGCTC G[AGT]GC[ACT]^C BspDI 823 ATCGAT AT^CGAT BspHI 284 TCATGA T^CATGA BstXI 217 CCACTAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 764 GCTGGC GC[ACGT]^[ACGT]GC CelII 324 GCTTAGC GC^T[ACGT]AGC Cfr13I 309 GGTCC G^G[ACGT]CC ClaI 823 ATCGAT AT^CGAT DraII 308 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 429 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 308 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRV 4 GATATC GAT^ATC EcoT14I 317 CCAAGG C^C[AT][AT]GG EcoT22I 550 ATGCAT ATGCA^T FauI 79 CCCGC CCCGC(4/6) HgaI 467 GACGC GACGC(5/10) KpnI 869 GGTACC GGTAC^C MaeI 220 CTAG C^TAG MluNI 881 TGGCCA TGG^CCA MscI 881 TGGCCA TGG^CCA MseI 757 TTAA T^TAA NsiI 550 ATGCAT ATGCA^T PaeR7I 929 CTCGAG C^TCGAG PflMI 873 CCATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 583 GACTC GAGTC(4/5) PpuMI 308 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 276 GACAACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 284 TCATGA T^CATGA Sau96I 309 GGTCC G^G[ACGT]CC SmlI 929 CTCGAG C^T[CT][GA]AG SspI 725 AATATT AAT^ATT StyI 317 CCAAGG C^C[AT][AT]GG Tru9I 757 TTAA T^TAA Van91I 873 CCATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 217 CCACTAGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 929 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 433 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 471 CAGATTCTG BanI 154 GGCACC G^G[CT][GA]CC 869 GGTACC BclI 287 TGATCA T^GATCA 830 TGATCA BcnI 168 CCCGG CC^[GC]GG 782 CCGGG BsmFI 465 GGGAC GGGAC(10/14) 310 GTCCC BsmI 548 GAATGC GAATGC(1/-1) 794 GCATTC Bsp1407I 297 TGTACA T^GTACA 972 TGTACA BsrGI 297 TGTACA T^GTACA 972 TGTACA EaeI 881 TGGCCA [CT]^GGCC[GA] 1003 TGGCCG EarI 673 CTCTTC CTCTTC(1/4) 906 CTCTTC FbaI 287 TGATCA T^GATCA 830 TGATCA HapII 169 CCGG C^CGG 782 CCGG HpaII 169 CCGG C^CGG 782 CCGG Ksp632I 673 CTCTTC CTCTTC(1/4) 906 CTCTTC MslI 218 CACTAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 513 CATTACAATG MspI 169 CCGG C^CGG 782 CCGG NciI 168 CCCGG CC^[GC]GG 782 CCGGG SspBI 297 TGTACA T^GTACA 972 TGTACA Tsp45I 683 GTCAC ^GT[GC]AC 737 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BsiYI BslI BsmAI BsrDI BsrI BssKI BstNI DdeI DpnII EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI TaqI TfiI TseI Tsp509I TspRI TthHB8I