(Circular) (Six-base) MAPSORT of: pFN21AE3132.seq Check: 8342 from: 1 to: 5713 pFN21AE3132 5713 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3523 278 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2372 3056 1937 Size: 435 684 4594 Fragments arranged by size: 4594 684 435 Acc65I G'GTAC_C Cuts at: 2399 3034 2399 Size: 635 5078 AclI AA'CG_TT Cuts at: 1563 3840 4213 1563 Size: 2277 373 3063 Fragments arranged by size: 3063 2277 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 2261 3776 5160 5428 1632 Size: 488 141 1515 1384 268 1917 Fragments arranged by size: 1917 1515 1384 488 268 141 AflII C'TTAA_G Cuts at: 820 1017 2150 2171 820 Size: 197 1133 21 4362 Fragments arranged by size: 4362 1133 197 21 AflIII A'CryG_T Cuts at: 1203 4612 5589 1203 Size: 3409 977 1327 Fragments arranged by size: 3409 1327 977 AgeI A'CCGG_T Cuts at: 4544 4544 Size: 5713 AhdI GACnn_n'nnGTC Cuts at: 4442 4442 Size: 5713 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2136 1184 Size: 952 4761 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2022 2054 2022 Size: 32 5681 AlwNI CAG_nnn'CTG Cuts at: 3456 5028 3456 Size: 1572 4141 ApaLI G'TGCA_C Cuts at: 3770 4926 3770 Size: 1156 4557 ApoI r'AATT_y Cuts at: 1493 2286 3022 3248 3400 5509 1493 Size: 793 736 226 152 2109 1697 Fragments arranged by size: 2109 1697 793 736 226 152 AseI AT'TA_AT Cuts at: 160 1032 4267 160 Size: 872 3235 1606 Fragments arranged by size: 3235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 AvaI C'yCGr_G Cuts at: 1870 1943 3038 1870 Size: 73 1095 4545 Fragments arranged by size: 4545 1095 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2373 2406 2373 Size: 33 5680 BamHI G'GATC_C Cuts at: 3043 3410 3043 Size: 367 5346 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2399 3034 4489 618 Size: 325 217 627 612 635 1455 1842 Fragments arranged by size: 1842 1455 635 627 612 325 217 BanII G_rGCy'C Cuts at: 1391 2986 3032 1391 Size: 1595 46 4072 Fragments arranged by size: 4072 1595 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4742 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2538 2572 3904 3938 1903 Size: 34 50 34 517 34 1332 34 3678 Fragments arranged by size: 3678 1332 517 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3605 4815 3605 Size: 1210 4503 BclI T'GATC_A Cuts at: 1548 1548 Size: 5713 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4324 136 Size: 107 122 71 631 605 2652 1525 Fragments arranged by size: 2652 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5708 5708 Size: 5713 BlpI GC'TnA_GC Cuts at: 3130 3130 Size: 5713 Bme1580I G_kGCm'C Cuts at: 1790 3774 4930 1790 Size: 1984 1156 2573 Fragments arranged by size: 2573 1984 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5713 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3444 4402 442 Size: 416 53 83 399 24 2027 958 1753 Fragments arranged by size: 2027 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5713 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2838 4373 1239 Size: 890 709 1535 2579 Fragments arranged by size: 2579 1535 890 709 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2730 3838 4703 5001 2730 Size: 1108 865 298 3442 Fragments arranged by size: 3442 1108 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4376 882 Size: 617 2877 2219 Fragments arranged by size: 2877 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3504 493 Size: 711 2300 2702 Fragments arranged by size: 2702 2300 711 BsaBI GATnn'nnATC Cuts at: 5707 5707 Size: 5713 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3520 3902 4537 275 Size: 53 83 186 936 1987 382 635 1451 Fragments arranged by size: 1987 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4146 4544 4818 4965 5376 4146 Size: 398 274 147 411 4483 Fragments arranged by size: 4483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5488 Fragments arranged by size: 5488 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2787 1186 Size: 1601 4112 BseYI C'CCAG_C Cuts at: 4916 5412 4916 Size: 496 5217 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2142 2318 1163 Size: 573 406 176 4558 Fragments arranged by size: 4558 573 406 176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3163 3924 4952 1540 Size: 275 186 1162 761 1028 2301 Fragments arranged by size: 2301 1162 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3774 3859 4930 3774 Size: 85 1071 4557 Fragments arranged by size: 4557 1071 85 BsmI GAATG_Cn' Cuts at: 3224 3317 3224 Size: 93 5620 BsmBI CGTCTCn'nnnn_ Cuts at: 2069 2424 2069 Size: 355 5358 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2986 3032 3774 3859 4930 1391 Size: 399 1196 46 742 85 1071 2174 Fragments arranged by size: 2174 1196 1071 742 399 85 46 BspEI T'CCGG_A Cuts at: 5376 5376 Size: 5713 BspHI T'CATG_A Cuts at: 2049 3602 5332 2049 Size: 1553 1730 2430 Fragments arranged by size: 2430 1730 1553 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2728 3068 844 Size: 1884 340 3489 Fragments arranged by size: 3489 1884 340 BsrBI CCG'CTC Cuts at: 1394 1823 3600 1394 Size: 429 1777 3507 Fragments arranged by size: 3507 1777 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2757 4208 4382 65 Size: 1183 1509 1451 174 1396 Fragments arranged by size: 1509 1451 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4357 4544 1700 Size: 110 2547 187 2869 Fragments arranged by size: 2869 2547 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5713 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5713 BssSI C'ACGA_G Cuts at: 3773 4785 3773 Size: 1012 4701 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5713 BstBI TT'CG_AA Cuts at: 2548 4517 2548 Size: 1969 3744 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5713 BstYI r'GATC_y Cuts at: 1972 2542 2895 3043 3410 3795 3812 5264 5350 Size: 570 353 148 367 385 17 1452 86 Cuts at: 5350 5362 5708 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 570 385 367 353 346 148 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2034 513 Size: 552 969 4192 Fragments arranged by size: 4192 969 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4974 Fragments arranged by size: 4974 683 56 BtsI GCAGTG_nn' Cuts at: 2330 3229 4023 4043 2330 Size: 899 794 20 4000 Fragments arranged by size: 4000 899 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5678 DraI TTT'AAA Cuts at: 3017 3017 Size: 5713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4720 809 Size: 507 3404 1802 Fragments arranged by size: 3404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3160 4049 5678 Size: 54 1425 50 275 18 1330 889 1629 Cuts at: 5678 8 Size: 43 Fragments arranged by size: 1629 1425 1330 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3160 1537 Size: 275 1348 4090 Fragments arranged by size: 4090 1348 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 2202 3643 1353 Size: 743 106 1441 3423 Fragments arranged by size: 3423 1441 743 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4300 4674 4820 387 Size: 1064 138 2711 374 146 1280 Fragments arranged by size: 2711 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2129 2261 2838 3776 4373 5160 Size: 393 488 9 132 577 938 597 787 Cuts at: 5160 5428 1239 Size: 268 1524 Fragments arranged by size: 1524 938 787 597 577 488 393 268 132 9 EcoRI G'AATT_C Cuts at: 3022 3400 3022 Size: 378 5335 FspI TGC'GCA Cuts at: 4219 4219 Size: 5713 HaeII r_GCGC'y Cuts at: 1678 4860 1678 Size: 3182 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2671 2703 4354 4386 4428 Size: 32 135 32 1099 32 1651 32 42 Cuts at: 4428 4460 1373 Size: 32 2626 Fragments arranged by size: 2626 1651 1099 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3057 3303 669 Size: 988 281 1119 246 3079 Fragments arranged by size: 3079 1119 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5713 HpaI GTT'AAC Cuts at: 3303 3303 Size: 5713 KpnI G_GTAC'C Cuts at: 2403 3038 2403 Size: 635 5078 MfeI C'AATT_G Cuts at: 3312 3312 Size: 5713 MluI A'CGCG_T Cuts at: 5589 5589 Size: 5713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2599 4827 5011 1305 Size: 1294 2228 184 2007 Fragments arranged by size: 2228 2007 1294 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3891 Fragments arranged by size: 3891 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2583 2916 3671 4030 4189 518 Size: 648 1417 333 755 359 159 2042 Fragments arranged by size: 2042 1417 755 648 359 333 159 MspA1I CmG'CkG Cuts at: 1606 3129 3806 4954 5199 1606 Size: 1523 677 1148 245 2120 Fragments arranged by size: 2120 1523 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5161 NdeI CA'TA_TG Cuts at: 387 387 Size: 5713 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5713 NotI GC'GGCC_GC Cuts at: 3160 3160 Size: 5713 NspI r_CATG'y Cuts at: 1132 1229 3071 4616 1132 Size: 97 1842 1545 2229 Fragments arranged by size: 2229 1842 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5713 PciI A'CATG_T Cuts at: 4612 4612 Size: 5713 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5713 PfoI T'CCnGG_A Cuts at: 2815 2815 Size: 5713 PmeI GTTT'AAAC Cuts at: 3017 3017 Size: 5713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 5295 5327 3782 Size: 32 1481 32 4168 Fragments arranged by size: 4168 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2642 1613 Size: 1029 4684 PsiI TTA'TAA Cuts at: 3283 3283 Size: 5713 PstI C_TGCA'G Cuts at: 830 3065 830 Size: 2235 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5713 SalI G'TCGA_C Cuts at: 1936 3055 1936 Size: 1119 4594 SbfI CC_TGCA'GG Cuts at: 3065 3065 Size: 5713 ScaI AGT'ACT Cuts at: 3961 5673 3961 Size: 1712 4001 SfcI C'TryA_G Cuts at: 826 1045 2373 3061 4196 4877 5068 826 Size: 219 1328 688 1135 681 191 1471 Fragments arranged by size: 1471 1328 1135 688 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5713 SmaI CCC'GGG Cuts at: 1872 3040 1872 Size: 1168 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 2171 2745 3817 4718 4980 Size: 197 926 207 21 574 1072 901 262 Cuts at: 4980 820 Size: 1553 Fragments arranged by size: 1553 1072 926 901 574 262 207 197 21 SnaBI TAC'GTA Cuts at: 493 3504 493 Size: 3011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5713 SphI G_CATG'C Cuts at: 1132 3071 1132 Size: 1939 3774 SspI AAT'ATT Cuts at: 5 52 3637 5 Size: 47 3585 2081 Fragments arranged by size: 3585 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5713 StyI C'CwwG_G Cuts at: 513 1065 2633 3152 513 Size: 552 1568 519 3074 Fragments arranged by size: 3074 1568 552 519 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3740 3757 3910 4095 4540 325 Size: 1255 2160 17 153 185 445 1498 Fragments arranged by size: 2160 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3959 5671 96 Size: 274 80 33 51 1443 1982 1712 138 Fragments arranged by size: 1982 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5673 5705 5673 Size: 32 5681 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5713 XbaI T'CTAG_A Cuts at: 3049 3049 Size: 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5713 XmaI C'CCGG_G Cuts at: 1870 3038 1870 Size: 1168 4545 XmnI GAAnn'nnTTC Cuts at: 3842 3842 Size: 5713 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3521 276 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI