################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AciI 679 CCGC CCGC(-3/-1) AlwNI 689 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 1138 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 119 CTCGGG C^[CT]CG[GA]G BanII 208 GAGCCC G[GA]GC[CT]^C BbvCI 1283 CCTCAGC CCTCAGC(-5/-2) BpmI 713 CTCCAG CTGGAG(16/14) BseRI 439 GAGGAG GAGGAG(10/8) BsiYI 606 CCCTCGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 606 CCCTCGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 60 GCATTC GAATGC(1/-1) BsoBI 119 CTCGGG C^[CT]CG[GA]G BstAPI 880 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 726 AGATCC [GA]^GATC[CT] DraI 1077 TTTAAA TTT^AAA DsaI 464 CCATGG C^C[GA][CT]GG HphI 582 GGTGA GGTGA(8/7) MflI 726 AGATCC [GA]^GATC[CT] MslI 465 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 716 CAGCTG C[AC]G^C[GT]G NcoI 464 CCATGG C^CATGG NlaIII 465 CATG CATG^ PflFI 1138 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 695 CTGCAG CTGCA^G PvuII 716 CAGCTG CAG^CTG ScaI 1243 AGTACT AGT^ACT SfcI 695 CTGCAG C^T[GA][CT]AG SmlI 1378 CTCAAG C^T[CT][GA]AG SpeI 859 ACTAGT A^CTAGT Tth111I 1138 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 726 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 173 AAATTT [GA]^AATT[CT] 825 AAATTC AlwI 28 GATCC GGATC(4/5) 727 GATCC ApoI 173 AAATTT [GA]^AATT[CT] 825 AAATTC Asp700I 824 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1028 GAATAGGTTC BbsI 227 GTCTTC GAAGAC(2/6) 1157 GTCTTC BmyI 208 GAGCCC G[AGT]GC[ACT]^C 626 GTGCCC BpuAI 227 GTCTTC GAAGAC(2/6) 1157 GTCTTC BsaI 294 GAGACC GGTCTC(1/5) 442 GAGACC Bsp1286I 208 GAGCCC G[AGT]GC[ACT]^C 626 GTGCCC BspMI 297 ACCTGC ACCTGC(4/8) 960 ACCTGC BstXI 464 CCATGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1197 CCAACTAAGTGG Cac8I 481 GCCTGC GC[ACGT]^[ACGT]GC 838 GCCAGC DpnII 28 GATC ^GATC 727 GATC DraII 254 AGGTCCT [GA]G^G[ACGT]CC[CT] 1230 AGGACCT EcoO109I 254 AGGTCCT [GA]G^G[ACGT]CC[CT] 1230 AGGACCT EcoT14I 464 CCATGG C^C[AT][AT]GG 1100 CCTTGG MboI 28 GATC ^GATC 727 GATC NdeII 28 GATC ^GATC 727 GATC PpuMI 254 AGGTCCT [GA]G^G[AT]CC[CT] 1230 AGGACCT Sau3AI 28 GATC ^GATC 727 GATC SfaNI 511 GCATC GCATC(5/9) 1288 GCATC StyI 464 CCATGG C^C[AT][AT]GG 1100 CCTTGG Tsp45I 583 GTGAC ^GT[GC]AC 1136 GTGAC XmnI 824 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1028 GAATAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AluI AvaII BbvI BfaI BsaJI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboII MnlI MseI MvaI MwoI NlaIV RsaI Sau96I ScrFI TfiI Tru9I TseI Tsp509I TspRI