(Circular) (Six-base) MAPSORT of: pFN21AE2296.seq Check: 9750 from: 1 to: 8200 pFN21AE2296 8200 bp With 182 enzymes: SgfI * November 4, 2010 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5050 5050 Size: 8200 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6010 278 Size: 53 83 186 936 4474 2468 Fragments arranged by size: 4474 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5543 1937 Size: 3606 4594 Acc65I G'GTAC_C Cuts at: 5521 5521 Size: 8200 AclI AA'CG_TT Cuts at: 1563 6327 6700 1563 Size: 4764 373 3063 Fragments arranged by size: 4764 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2309 2525 3246 3647 3758 4110 4668 5120 Size: 677 216 721 401 111 352 558 452 Cuts at: 5120 5250 5457 6263 7647 7915 1632 Size: 130 207 806 1384 268 1917 Fragments arranged by size: 1917 1384 806 721 677 558 452 401 352 268 216 207 130 111 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8003 AflIII A'CryG_T Cuts at: 1203 3406 7099 8076 1203 Size: 2203 3693 977 1327 Fragments arranged by size: 3693 2203 1327 977 AgeI A'CCGG_T Cuts at: 7031 7031 Size: 8200 AhdI GACnn_n'nnGTC Cuts at: 6929 6929 Size: 8200 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8200 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3098 1184 Size: 1914 6286 AlwNI CAG_nnn'CTG Cuts at: 2548 2645 4030 4133 5098 5943 7515 2548 Size: 97 1385 103 965 845 1572 3233 Fragments arranged by size: 3233 1572 1385 965 845 103 97 ApaLI G'TGCA_C Cuts at: 3983 6257 7413 3983 Size: 2274 1156 4770 Fragments arranged by size: 4770 2274 1156 ApoI r'AATT_y Cuts at: 1493 3056 3826 4450 5356 5464 5509 5735 5887 Size: 1563 770 624 906 108 45 226 152 Cuts at: 5887 7996 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1563 906 770 624 226 152 108 45 AseI AT'TA_AT Cuts at: 160 1032 6754 160 Size: 872 5722 1606 Fragments arranged by size: 5722 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8200 AvaI C'yCGr_G Cuts at: 1870 1943 5525 1870 Size: 73 3582 4545 Fragments arranged by size: 4545 3582 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3887 3920 5358 5391 3887 Size: 33 1438 33 6696 Fragments arranged by size: 6696 1438 33 33 BamHI G'GATC_C Cuts at: 5530 5897 5530 Size: 367 7833 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3373 5521 6976 618 Size: 325 217 627 1586 2148 1455 1842 Fragments arranged by size: 2148 1842 1586 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2137 2322 3849 5519 1391 Size: 746 185 1527 1670 4072 Fragments arranged by size: 4072 1670 1527 746 185 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7229 BbvCI CC'TCA_GC Cuts at: 3252 3252 Size: 8200 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6391 6425 1903 Size: 34 4454 34 3678 Fragments arranged by size: 4454 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5359 6092 7302 5359 Size: 733 1210 6257 Fragments arranged by size: 6257 1210 733 BclI T'GATC_A Cuts at: 1548 4306 1548 Size: 2758 5442 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3844 6811 136 Size: 107 122 71 631 605 2172 2967 1525 Fragments arranged by size: 2967 2172 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3655 4633 8195 3655 Size: 978 3562 3660 Fragments arranged by size: 3660 3562 978 BlpI GC'TnA_GC Cuts at: 4749 5617 4749 Size: 868 7332 Bme1580I G_kGCm'C Cuts at: 1790 3666 3987 6261 7417 1790 Size: 1876 321 2274 1156 2573 Fragments arranged by size: 2573 2274 1876 1156 321 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8200 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4088 5931 6889 Size: 416 53 83 399 24 2671 1843 958 Cuts at: 6889 442 Size: 1753 Fragments arranged by size: 2671 1843 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3044 1056 Size: 1988 6212 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 3905 3941 4259 4442 6860 1239 Size: 851 1815 36 318 183 2418 2579 Fragments arranged by size: 2579 2418 1815 851 318 183 36 Bpu10I CC'TnA_GC Cuts at: 3252 3252 Size: 8200 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3524 6325 7190 7488 3524 Size: 2801 865 298 4236 Fragments arranged by size: 4236 2801 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5075 6863 882 Size: 617 3576 1788 2219 Fragments arranged by size: 3576 2219 1788 617 BsaAI yAC'GTr Cuts at: 493 1204 5991 493 Size: 711 4787 2702 Fragments arranged by size: 4787 2702 711 BsaBI GATnn'nnATC Cuts at: 8194 8194 Size: 8200 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6007 6389 7024 275 Size: 53 83 186 936 4474 382 635 1451 Fragments arranged by size: 4474 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6633 7031 7305 7452 7863 6633 Size: 398 274 147 411 6970 Fragments arranged by size: 6970 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3878 3908 4655 4685 1178 Size: 30 165 30 2475 30 747 30 4693 Fragments arranged by size: 4693 2475 747 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3222 3225 1186 Size: 2036 3 6161 Fragments arranged by size: 6161 2036 3 BseYI C'CCAG_C Cuts at: 5239 7403 7899 5239 Size: 2164 496 5540 Fragments arranged by size: 5540 2164 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2750 4043 5081 1163 Size: 573 1014 1293 1038 4282 Fragments arranged by size: 4282 1293 1038 1014 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5650 6411 7439 1540 Size: 275 186 3649 761 1028 2301 Fragments arranged by size: 3649 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2137 3891 3987 6261 6346 7417 2137 Size: 1754 96 2274 85 1071 2920 Fragments arranged by size: 2920 2274 1754 1071 96 85 BsmI GAATG_Cn' Cuts at: 5711 5804 5711 Size: 93 8107 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2322 3666 3849 3891 3987 5519 Size: 399 347 185 1344 183 42 96 1532 Cuts at: 5519 6261 6346 7417 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1532 1344 1071 742 399 347 185 183 96 85 42 BspEI T'CCGG_A Cuts at: 7863 7863 Size: 8200 BspHI T'CATG_A Cuts at: 6089 7819 6089 Size: 1730 6470 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5050 5555 844 Size: 4206 505 3489 Fragments arranged by size: 4206 3489 505 BsrBI CCG'CTC Cuts at: 1394 1823 6087 1394 Size: 429 4264 3507 Fragments arranged by size: 4264 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2721 2937 6695 6869 65 Size: 1183 1473 216 3758 174 1396 Fragments arranged by size: 3758 1473 1396 1183 216 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6844 7031 1700 Size: 110 5034 187 2869 Fragments arranged by size: 5034 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8200 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8200 BssSI C'ACGA_G Cuts at: 3651 6260 7272 3651 Size: 2609 1012 4579 Fragments arranged by size: 4579 2609 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4597 794 Size: 3803 4397 BstBI TT'CG_AA Cuts at: 7004 7004 Size: 8200 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8200 BstYI r'GATC_y Cuts at: 1972 3655 4633 5530 5897 6282 6299 7751 7837 Size: 1683 978 897 367 385 17 1452 86 Cuts at: 7837 7849 8195 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1683 1452 978 897 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2815 3369 2815 Size: 554 7646 BtgI C'CryG_G Cuts at: 513 1065 2004 2655 5085 513 Size: 552 939 651 2430 3628 Fragments arranged by size: 3628 2430 939 651 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2790 487 Size: 683 56 1564 5897 Fragments arranged by size: 5897 1564 683 56 BtsI GCAGTG_nn' Cuts at: 2120 2524 2622 2880 3574 3758 5211 5716 6510 Size: 404 98 258 694 184 1453 505 794 Cuts at: 6510 6530 2120 Size: 20 3790 Fragments arranged by size: 3790 1453 794 694 505 404 258 184 98 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8200 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4932 4967 529 Size: 35 4368 35 3762 Fragments arranged by size: 4368 3762 35 35 DraI TTT'AAA Cuts at: 3117 5504 3117 Size: 2387 5813 DraIII CAC_nnn'GTG Cuts at: 2163 2672 3982 4034 2163 Size: 509 1310 52 6329 Fragments arranged by size: 6329 1310 509 52 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7207 809 Size: 507 5891 1802 Fragments arranged by size: 5891 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4333 5647 6536 Size: 54 1425 50 275 18 2503 1314 889 Cuts at: 6536 8165 8 Size: 1629 43 Fragments arranged by size: 2503 1629 1425 1314 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5647 1537 Size: 275 3835 4090 Fragments arranged by size: 4090 3835 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2656 3243 6130 1353 Size: 1303 587 2887 3423 Fragments arranged by size: 3423 2887 1303 587 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6787 7161 7307 387 Size: 1064 138 5198 374 146 1280 Fragments arranged by size: 5198 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2135 2135 Size: 8200 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2309 2525 3246 3647 3758 3905 Size: 393 458 219 216 721 401 111 147 Cuts at: 3905 3941 4110 4259 4442 4668 5120 5250 5457 Size: 36 169 149 183 226 452 130 207 Cuts at: 5457 6263 6860 7647 7915 1239 Size: 806 597 787 268 1524 Fragments arranged by size: 1524 806 787 721 597 458 452 401 393 268 226 219 216 207 183 169 149 147 130 111 36 EcoNI CCTnn'n_nnAGG Cuts at: 3264 3264 Size: 8200 EcoO109I rG'GnC_Cy Cuts at: 3709 3709 Size: 8200 EcoRI G'AATT_C Cuts at: 3056 5509 5887 3056 Size: 2453 378 5369 Fragments arranged by size: 5369 2453 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3193 3876 4655 3193 Size: 683 779 6738 Fragments arranged by size: 6738 779 683 FspI TGC'GCA Cuts at: 4740 6706 4740 Size: 1966 6234 HaeII r_GCGC'y Cuts at: 1678 7347 1678 Size: 5669 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2074 2106 2122 2154 3379 Size: 32 135 32 502 32 16 32 1225 Cuts at: 3379 3411 3878 3910 4198 4230 4270 4302 4537 Size: 32 467 32 288 32 40 32 235 Cuts at: 4537 4569 4655 4687 5381 5413 6841 6873 6915 Size: 32 86 32 694 32 1428 32 42 Cuts at: 6915 6947 1373 Size: 32 2626 Fragments arranged by size: 2626 1428 1225 694 502 467 288 235 135 86 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 2417 4730 5544 5790 669 Size: 988 281 479 2313 814 246 3079 Fragments arranged by size: 3079 2313 988 814 479 281 246 HindIII A'AGCT_T Cuts at: 748 3858 4680 748 Size: 3110 822 4268 Fragments arranged by size: 4268 3110 822 HpaI GTT'AAC Cuts at: 5790 5790 Size: 8200 KpnI G_GTAC'C Cuts at: 5525 5525 Size: 8200 MfeI C'AATT_G Cuts at: 3212 5799 3212 Size: 2587 5613 MluI A'CGCG_T Cuts at: 8076 8076 Size: 8200 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3855 7314 7498 1305 Size: 2550 3459 184 2007 Fragments arranged by size: 3459 2550 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4335 10 Size: 54 1425 343 2503 3875 Fragments arranged by size: 3875 2503 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2667 2726 2751 3409 4773 4779 6158 Size: 648 1501 59 25 658 1364 6 1379 Cuts at: 6158 6517 6676 518 Size: 359 159 2042 Fragments arranged by size: 2042 1501 1379 1364 658 648 359 159 59 25 6 MspA1I CmG'CkG Cuts at: 1606 2870 3883 5294 5616 6293 7441 7686 1606 Size: 1264 1013 1411 322 677 1148 245 2120 Fragments arranged by size: 2120 1411 1264 1148 1013 677 322 245 NcoI C'CATG_G Cuts at: 513 1065 2004 5085 513 Size: 552 939 3081 3628 Fragments arranged by size: 3628 3081 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8200 NheI G'CTAG_C Cuts at: 1052 3040 1052 Size: 1988 6212 NotI GC'GGCC_GC Cuts at: 5647 5647 Size: 8200 NspI r_CATG'y Cuts at: 1132 1229 3410 3838 4488 4600 4853 4976 5558 Size: 97 2181 428 650 112 253 123 582 Cuts at: 5558 7103 1132 Size: 1545 2229 Fragments arranged by size: 2229 2181 1545 650 582 428 253 123 112 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8200 PciI A'CATG_T Cuts at: 3406 7099 3406 Size: 3693 4507 PflMI CCAn_nnn'nTGG Cuts at: 1108 3560 1108 Size: 2452 5748 PfoI T'CCnGG_A Cuts at: 2679 2679 Size: 8200 PmeI GTTT'AAAC Cuts at: 5504 5504 Size: 8200 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2295 2327 6269 6301 7782 7814 2295 Size: 32 3942 32 1481 32 2681 Fragments arranged by size: 3942 2681 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3526 1613 Size: 1913 6287 PsiI TTA'TAA Cuts at: 5770 5770 Size: 8200 PstI C_TGCA'G Cuts at: 830 2519 2875 3013 3484 5221 5552 830 Size: 1689 356 138 471 1737 331 3478 Fragments arranged by size: 3478 1737 1689 471 356 331 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8200 PvuII CAG'CTG Cuts at: 2870 3883 5294 2870 Size: 1013 1411 5776 Fragments arranged by size: 5776 1411 1013 SacI G_AGCT'C Cuts at: 2137 2137 Size: 8200 SalI G'TCGA_C Cuts at: 1936 5542 1936 Size: 3606 4594 SbfI CC_TGCA'GG Cuts at: 5552 5552 Size: 8200 ScaI AGT'ACT Cuts at: 6448 8160 6448 Size: 1712 6488 SexAI A'CCwGG_T Cuts at: 3978 3978 Size: 8200 SfcI C'TryA_G Cuts at: 826 1045 2463 2515 2637 2871 3009 3480 3578 Size: 219 1418 52 122 234 138 471 98 Cuts at: 3578 4691 5217 5548 6683 7364 7555 826 Size: 1113 526 331 1135 681 191 1471 Fragments arranged by size: 1471 1418 1135 1113 681 526 471 331 234 219 191 138 122 98 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8200 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8200 SmaI CCC'GGG Cuts at: 1872 5527 1872 Size: 3655 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3539 6304 7205 7467 820 Size: 197 926 1596 2765 901 262 1553 Fragments arranged by size: 2765 1596 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5991 493 Size: 5498 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8200 SphI G_CATG'C Cuts at: 1132 4488 4853 5558 1132 Size: 3356 365 705 3774 Fragments arranged by size: 3774 3356 705 365 SspI AAT'ATT Cuts at: 5 52 2702 4604 6124 5 Size: 47 2650 1902 1520 2081 Fragments arranged by size: 2650 2081 1902 1520 47 StuI AGG'CCT Cuts at: 1517 2888 3442 1517 Size: 1371 554 6275 Fragments arranged by size: 6275 1371 554 StyI C'CwwG_G Cuts at: 513 1065 2004 3851 3934 5085 5639 513 Size: 552 939 1847 83 1151 554 3074 Fragments arranged by size: 3074 1847 1151 939 554 552 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2817 3881 6227 6244 6397 6582 7027 Size: 1255 1237 1064 2346 17 153 185 445 Cuts at: 7027 325 Size: 1498 Fragments arranged by size: 2346 1498 1255 1237 1064 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4429 5429 6446 Size: 274 80 33 51 1443 2452 1000 1017 Cuts at: 6446 8158 96 Size: 1712 138 Fragments arranged by size: 2452 1712 1443 1017 1000 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8160 8192 8160 Size: 32 8168 Tth111I GACn'n_nGTC Cuts at: 1316 2046 1316 Size: 730 7470 XbaI T'CTAG_A Cuts at: 5536 5536 Size: 8200 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2357 4721 2357 Size: 2364 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8200 XmaI C'CCGG_G Cuts at: 1870 5525 1870 Size: 3655 4545 XmnI GAAnn'nnTTC Cuts at: 4356 6329 4356 Size: 1973 6227 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6008 276 Size: 53 83 186 936 4474 2468 Fragments arranged by size: 4474 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI