(Linear) (Six-base) MAPSORT of: KIEE2296.seq Check: 139 from: 1 to: 3495 KIEE2296.orf With 182 enzymes: SgfI * November 2, 2010 17:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3045 3495 Size: 3045 450 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 520 1241 1642 1753 2105 2663 3115 Size: 304 216 721 401 111 352 558 452 Cuts at: 3115 3245 3452 3495 Size: 130 207 43 Fragments arranged by size: 721 558 452 401 352 304 216 207 130 111 43 AflIII A'CryG_T Cuts at: 0 1401 3495 Size: 1401 2094 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1093 3495 Size: 1093 2402 AlwNI CAG_nnn'CTG Cuts at: 0 543 640 2025 2128 3093 3495 Size: 543 97 1385 103 965 402 Fragments arranged by size: 1385 965 543 402 103 97 ApaLI G'TGCA_C Cuts at: 0 1978 3495 Size: 1978 1517 ApoI r'AATT_y Cuts at: 0 1051 1821 2445 3351 3459 3495 Size: 1051 770 624 906 108 36 Fragments arranged by size: 1051 906 770 624 108 36 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1882 1915 3353 3386 3495 Size: 1882 33 1438 33 109 Fragments arranged by size: 1882 1438 109 33 33 BanI G'GyrC_C Cuts at: 0 1368 3495 Size: 1368 2127 BanII G_rGCy'C Cuts at: 0 132 317 1844 3495 Size: 132 185 1527 1651 Fragments arranged by size: 1651 1527 185 132 BbvCI CC'TCA_GC Cuts at: 0 1247 3495 Size: 1247 2248 BciVI GTATCCnnnnn_n' Cuts at: 0 3354 3495 Size: 3354 141 BclI T'GATC_A Cuts at: 0 2301 3495 Size: 2301 1194 BglI GCCn_nnn'nGGC Cuts at: 0 1839 3495 Size: 1839 1656 BglII A'GATC_T Cuts at: 0 1650 2628 3495 Size: 1650 978 867 Fragments arranged by size: 1650 978 867 BlpI GC'TnA_GC Cuts at: 0 2744 3495 Size: 2744 751 Bme1580I G_kGCm'C Cuts at: 0 1661 1982 3495 Size: 1661 321 1513 Fragments arranged by size: 1661 1513 321 BmrI ACTGGGnnnn_n' Cuts at: 0 2083 3495 Size: 2083 1412 BmtI G_CTAG'C Cuts at: 0 1039 3495 Size: 1039 2456 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 1900 1936 2254 2437 3495 Size: 85 1815 36 318 183 1058 Fragments arranged by size: 1815 1058 318 183 85 36 Bpu10I CC'TnA_GC Cuts at: 0 1247 3495 Size: 1247 2248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1519 3495 Size: 1519 1976 BsaI GGTCTCn'nnnn_ Cuts at: 0 3070 3495 Size: 3070 425 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1873 1903 2650 2680 3495 Size: 1873 30 747 30 815 Fragments arranged by size: 1873 815 747 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1217 1220 3495 Size: 1217 3 2275 Fragments arranged by size: 2275 1217 3 BseYI C'CCAG_C Cuts at: 0 3234 3495 Size: 3234 261 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 745 2038 3076 3495 Size: 745 1293 1038 419 Fragments arranged by size: 1293 1038 745 419 BsiHKAI G_wGCw'C Cuts at: 0 132 1886 1982 3495 Size: 132 1754 96 1513 Fragments arranged by size: 1754 1513 132 96 Bsp1286I G_dGCh'C Cuts at: 0 132 317 1661 1844 1886 1982 3495 Size: 132 185 1344 183 42 96 1513 Fragments arranged by size: 1513 1344 185 183 132 96 42 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3045 3495 Size: 3045 450 BsrDI GCAATG_nn' Cuts at: 0 716 932 3495 Size: 716 216 2563 Fragments arranged by size: 2563 716 216 BssSI C'ACGA_G Cuts at: 0 1646 3495 Size: 1646 1849 BstAPI GCAn_nnn'nTGC Cuts at: 0 2592 3495 Size: 2592 903 BstYI r'GATC_y Cuts at: 0 1650 2628 3495 Size: 1650 978 867 Fragments arranged by size: 1650 978 867 Bsu36I CC'TnA_GG Cuts at: 0 810 1364 3495 Size: 810 554 2131 Fragments arranged by size: 2131 810 554 BtgI C'CryG_G Cuts at: 0 650 3080 3495 Size: 650 2430 415 Fragments arranged by size: 2430 650 415 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 785 3495 Size: 785 2710 BtsI GCAGTG_nn' Cuts at: 0 115 519 617 875 1569 1753 3206 3495 Size: 115 404 98 258 694 184 1453 289 Fragments arranged by size: 1453 694 404 289 258 184 115 98 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2927 2962 3495 Size: 2927 35 533 Fragments arranged by size: 2927 533 35 DraI TTT'AAA Cuts at: 0 1112 3495 Size: 1112 2383 DraIII CAC_nnn'GTG Cuts at: 0 158 667 1977 2029 3495 Size: 158 509 1310 52 1466 Fragments arranged by size: 1466 1310 509 158 52 EaeI y'GGCC_r Cuts at: 0 2328 3495 Size: 2328 1167 EarI CTCTTCn'nnn_ Cuts at: 0 651 1238 3495 Size: 651 587 2257 Fragments arranged by size: 2257 651 587 EcoICRI GAG'CTC Cuts at: 0 130 3495 Size: 130 3365 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 304 520 1241 1642 1753 1900 1936 Size: 85 219 216 721 401 111 147 36 Cuts at: 1936 2105 2254 2437 2663 3115 3245 3452 3495 Size: 169 149 183 226 452 130 207 43 Fragments arranged by size: 721 452 401 226 219 216 207 183 169 149 147 130 111 85 43 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 1259 3495 Size: 1259 2236 EcoO109I rG'GnC_Cy Cuts at: 0 1704 3495 Size: 1704 1791 EcoRI G'AATT_C Cuts at: 0 1051 3495 Size: 1051 2444 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1188 1871 2650 3495 Size: 1188 683 779 845 Fragments arranged by size: 1188 845 779 683 FspI TGC'GCA Cuts at: 0 2735 3495 Size: 2735 760 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 69 101 117 149 1374 1406 1873 1905 Size: 69 32 16 32 1225 32 467 32 Cuts at: 1905 2193 2225 2265 2297 2532 2564 2650 2682 Size: 288 32 40 32 235 32 86 32 Cuts at: 2682 3376 3408 3495 Size: 694 32 87 Fragments arranged by size: 1225 694 467 288 235 87 86 69 40 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 0 412 2725 3495 Size: 412 2313 770 Fragments arranged by size: 2313 770 412 HindIII A'AGCT_T Cuts at: 0 1853 2675 3495 Size: 1853 822 820 Fragments arranged by size: 1853 822 820 MfeI C'AATT_G Cuts at: 0 1207 3495 Size: 1207 2288 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1850 3495 Size: 1850 1645 MscI TGG'CCA Cuts at: 0 2330 3495 Size: 2330 1165 MslI CAynn'nnrTG Cuts at: 0 662 721 746 1404 2768 2774 3495 Size: 662 59 25 658 1364 6 721 Fragments arranged by size: 1364 721 662 658 59 25 6 MspA1I CmG'CkG Cuts at: 0 865 1878 3289 3495 Size: 865 1013 1411 206 Fragments arranged by size: 1411 1013 865 206 NcoI C'CATG_G Cuts at: 0 3080 3495 Size: 3080 415 NheI G'CTAG_C Cuts at: 0 1035 3495 Size: 1035 2460 NspI r_CATG'y Cuts at: 0 1405 1833 2483 2595 2848 2971 3495 Size: 1405 428 650 112 253 123 524 Fragments arranged by size: 1405 650 524 428 253 123 112 PciI A'CATG_T Cuts at: 0 1401 3495 Size: 1401 2094 PflMI CCAn_nnn'nTGG Cuts at: 0 1555 3495 Size: 1555 1940 PfoI T'CCnGG_A Cuts at: 0 674 3495 Size: 674 2821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 290 322 3495 Size: 290 32 3173 Fragments arranged by size: 3173 290 32 PshAI GACnn'nnGTC Cuts at: 0 1521 3495 Size: 1521 1974 PstI C_TGCA'G Cuts at: 0 514 870 1008 1479 3216 3495 Size: 514 356 138 471 1737 279 Fragments arranged by size: 1737 514 471 356 279 138 PvuII CAG'CTG Cuts at: 0 865 1878 3289 3495 Size: 865 1013 1411 206 Fragments arranged by size: 1411 1013 865 206 SacI G_AGCT'C Cuts at: 0 132 3495 Size: 132 3363 SexAI A'CCwGG_T Cuts at: 0 1973 3495 Size: 1973 1522 SfcI C'TryA_G Cuts at: 0 458 510 632 866 1004 1475 1573 2686 Size: 458 52 122 234 138 471 98 1113 Cuts at: 2686 3212 3495 Size: 526 283 Fragments arranged by size: 1113 526 471 458 283 234 138 122 98 52 SmlI C'TyrA_G Cuts at: 0 1534 3495 Size: 1534 1961 SphI G_CATG'C Cuts at: 0 2483 2848 3495 Size: 2483 365 647 Fragments arranged by size: 2483 647 365 SspI AAT'ATT Cuts at: 0 697 2599 3495 Size: 697 1902 896 Fragments arranged by size: 1902 896 697 StuI AGG'CCT Cuts at: 0 883 1437 3495 Size: 883 554 2058 Fragments arranged by size: 2058 883 554 StyI C'CwwG_G Cuts at: 0 1846 1929 3080 3495 Size: 1846 83 1151 415 Fragments arranged by size: 1846 1151 415 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 812 1876 3495 Size: 812 1064 1619 Fragments arranged by size: 1619 1064 812 TatI w'GTAC_w Cuts at: 0 2424 3424 3495 Size: 2424 1000 71 Fragments arranged by size: 2424 1000 71 Tth111I GACn'n_nGTC Cuts at: 0 41 3495 Size: 41 3454 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 352 2716 3495 Size: 352 2364 779 Fragments arranged by size: 2364 779 352 XmnI GAAnn'nnTTC Cuts at: 0 2351 3495 Size: 2351 1144 Enzymes that do cut: AarI AcuI AflIII AlfI AlwNI ApaLI ApoI BaeI BanI BanII BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NheI NspI PciI PflMI PfoI PpiI PshAI PstI PvuII SacI SexAI SfcI SmlI SphI SspI StuI StyI TaqII TatI Tth111I XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BamHI BbeI BbsI BcgI BfrBI BmgBI BplI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I ClaI DrdI EagI EciI EcoRV FseI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PmeI PmlI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TstI XbaI XhoI XmaI ZraI