Restriction map for
pFN21AE2401
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrGI BsrI BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EcoO65I EcoRI
EcoRV FbaI FokI FseI FspI
HincII HindII HindIII HpaI KpnI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NheI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfuI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcsI 1009 AAATTT [GA]^AATT[CT]
AlwI 453 GATCC GGATC(4/5)
AlwNI 529 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 872 CCTCAGG CC^T[ACGT]AGG
ApoI 1009 AAATTT [GA]^AATT[CT]
AvaII 500 GGACC G^G[AT]CC
BanI 163 GGCGCC G^G[CT][GA]CC
BbeI 163 GGCGCC GGCGC^C
BbvCI 788 CCTCAGC CCTCAGC(-5/-2)
BfaI 1054 CTAG C^TAG
BglII 1001 AGATCT A^GATCT
BpmI 743 CTCCAG CTGGAG(16/14)
BsiEI 236 CGGCCG CG[GA][CT]^CG
BspHI 758 TCATGA T^CATGA
BsrBI 126 CCGCTC CCGCTC(-3/-3)
BsrFI 463 GCCGGC [GA]^CCGG[CT]
BstAPI 968 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1001 AGATCT [GA]^GATC[CT]
Bsu36I 872 CCTCAGG CC^T[ACGT]AGG
Cfr10I 463 GCCGGC [GA]^CCGG[CT]
DraII 41 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 233 CCGCGG C^C[GA][CT]GG
EagI 236 CGGCCG C^GGCCG
EclXI 236 CGGCCG C^GGCCG
Eco52I 236 CGGCCG C^GGCCG
Eco81I 872 CCTCAGG CC^T[ACGT]AGG
EcoNI 938 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 41 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 56 CCAAGG C^C[AT][AT]GG
EcoT22I 636 ATGCAT ATGCA^T
HgaI 962 GCGTC GACGC(5/10)
HphI 197 TCACC GGTGA(8/7)
KasI 163 GGCGCC G^GCGCC
KspI 233 CCGCGG CCGC^GG
MaeI 1054 CTAG C^TAG
MaeIII 799 GTCAC ^GT[ACGT]AC
MflI 1001 AGATCT [GA]^GATC[CT]
NaeI 463 GCCGGC GCC^GGC
NarI 163 GGCGCC GG^CGCC
NgoMIV 463 GCCGGC G^CCGGC
NotI 235 GCGGCCGC GC^GGCCGC
NsiI 636 ATGCAT ATGCA^T
PleI 523 GACTC GAGTC(4/5)
RcaI 758 TCATGA T^CATGA
SacII 233 CCGCGG CCGC^GG
SapI 1024 GAAGAGC GCTCTTC(1/4)
SfoI 163 GGCGCC GGC^GCC
SgrAI 462 CGCCGGCG C[GA]^CCGG[CT]G
SphI 506 GCATGC GCATG^C
StyI 56 CCAAGG C^C[AT][AT]GG
Tsp45I 799 GTCAC ^GT[GC]AC
XcmI 8 CCAATGTCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1001 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 163 GGCGCC G[GA]^CG[CT]C
961 GGCGTC
Aor51HI 119 AGCGCT AGC^GCT
1075 AGCGCT
BglI 229 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
823 GCCAATCTGGC
BsaHI 163 GGCGCC G[GA]^CG[CT]C
961 GGCGTC
BsmI 634 GAATGC GAATGC(1/-1)
1084 GCATTC
BssHII 190 GCGCGC G^CGCGC
244 GCGCGC
DdeI 789 CTCAG C^T[ACGT]AG
873 CTCAG
EarI 909 GAAGAG CTCTTC(1/4)
1024 GAAGAG
Eco47III 119 AGCGCT AGC^GCT
1075 AGCGCT
Hin1I 163 GGCGCC G[GA]^CG[CT]C
961 GGCGTC
Ksp632I 909 GAAGAG CTCTTC(1/4)
1024 GAAGAG
MspA1I 233 CCGCGG C[AC]G^C[GT]G
318 CAGCGG
NspI 254 GCATGT [GA]CATG^[CT]
506 GCATGC
PflMI 305 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
528 CCAGAGGCTGG
PstI 615 CTGCAG CTGCA^G
966 CTGCAG
SfcI 615 CTGCAG C^T[GA][CT]AG
966 CTGCAG
SmaI 25 CCCGGG CCC^GGG
45 CCCGGG
TfiI 681 GAATC G^A[AT]TC
846 GAATC
TspRI 613 CACTG CA[GC]TG[ACGT][ACGT]^
807 CAGTG
Van91I 305 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
528 CCAGAGGCTGG
XmaCI 25 CCCGGG C^CCGGG
45 CCCGGG
XmaI 25 CCCGGG C^CCGGG
45 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DpnII EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TseI Tsp509I