(Circular) (Six-base) MAPSORT of: pFN21AE2401.seq Check: 4734 from: 1 to: 5806 pFN21AE2401 5806 bp With 182 enzymes: SgfI * March 8, 2012 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3149 1937 Size: 1212 4594 Acc65I G'GTAC_C Cuts at: 3127 3127 Size: 5806 AclI AA'CG_TT Cuts at: 1563 3933 4306 1563 Size: 2370 373 3063 Fragments arranged by size: 3063 2370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2691 3105 3869 5253 5521 1632 Size: 1059 414 764 1384 268 1917 Fragments arranged by size: 1917 1384 1059 764 414 268 AfeI AGC'GCT Cuts at: 2126 3082 2126 Size: 956 4850 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 4705 5682 1203 Size: 3502 977 1327 Fragments arranged by size: 3502 1327 977 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 4535 4535 Size: 5806 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5806 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3058 3090 3058 Size: 32 5774 AlwNI CAG_nnn'CTG Cuts at: 2539 3549 5121 2539 Size: 1010 1572 3224 Fragments arranged by size: 3224 1572 1010 ApaLI G'TGCA_C Cuts at: 3863 5019 3863 Size: 1156 4650 ApoI r'AATT_y Cuts at: 1493 3014 3115 3341 3493 5602 1493 Size: 1521 101 226 152 2109 1697 Fragments arranged by size: 2109 1697 1521 226 152 101 AseI AT'TA_AT Cuts at: 160 1032 4360 160 Size: 872 3328 1606 Fragments arranged by size: 3328 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2050 2078 2693 3131 1870 Size: 73 87 20 28 615 438 4545 Fragments arranged by size: 4545 615 438 87 73 28 20 BamHI G'GATC_C Cuts at: 3136 3503 3136 Size: 367 5439 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 3127 4582 618 Size: 325 217 627 381 959 1455 1842 Fragments arranged by size: 1842 1455 959 627 381 325 217 BanII G_rGCy'C Cuts at: 1391 2031 2042 2089 3125 1391 Size: 640 11 47 1036 4072 Fragments arranged by size: 4072 1036 640 47 11 BbeI G_GCGC'C Cuts at: 2172 2172 Size: 5806 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4835 BbvCI CC'TCA_GC Cuts at: 2794 2794 Size: 5806 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3997 4031 1903 Size: 34 2060 34 3678 Fragments arranged by size: 3678 2060 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3698 4908 3698 Size: 1210 4596 BclI T'GATC_A Cuts at: 1548 1548 Size: 5806 BfrBI ATG'CAT Cuts at: 2643 2643 Size: 5806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2240 2834 4417 Size: 107 122 71 631 605 568 594 1583 Cuts at: 4417 136 Size: 1525 Fragments arranged by size: 1583 1525 631 605 594 568 122 107 71 BglII A'GATC_T Cuts at: 3006 5801 3006 Size: 2795 3011 BlpI GC'TnA_GC Cuts at: 3223 3223 Size: 5806 Bme1580I G_kGCm'C Cuts at: 1790 2943 3867 5023 1790 Size: 1153 924 1156 2573 Fragments arranged by size: 2573 1156 1153 924 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5806 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3537 4495 442 Size: 416 53 83 399 24 2120 958 1753 Fragments arranged by size: 2120 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2733 4466 1239 Size: 1494 1733 2579 Fragments arranged by size: 2579 1733 1494 Bpu10I CC'TnA_GC Cuts at: 2794 2794 Size: 5806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3931 4796 5094 3931 Size: 865 298 4643 Fragments arranged by size: 4643 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4469 882 Size: 617 2970 2219 Fragments arranged by size: 2970 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3597 493 Size: 711 2393 2702 Fragments arranged by size: 2702 2393 711 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 2967 3613 3995 Size: 53 83 186 936 636 798 646 382 Cuts at: 3995 4630 275 Size: 635 1451 Fragments arranged by size: 1451 936 798 646 636 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4239 4637 4911 5058 5469 4239 Size: 398 274 147 411 4576 Fragments arranged by size: 4576 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5581 Fragments arranged by size: 5581 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2407 2422 2425 2434 2437 2440 2851 2854 Size: 1221 15 3 9 3 3 411 3 Cuts at: 2854 2857 2866 1186 Size: 3 9 4126 Fragments arranged by size: 4126 1221 411 15 9 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2219 2539 2902 3077 5009 5505 2219 Size: 320 363 175 1932 496 2520 Fragments arranged by size: 2520 1932 496 363 320 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5233 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2244 3256 4017 5045 1540 Size: 275 186 243 1012 761 1028 2301 Fragments arranged by size: 2301 1028 1012 761 275 243 186 BsiHKAI G_wGCw'C Cuts at: 3867 3952 5023 3867 Size: 85 1071 4650 Fragments arranged by size: 4650 1071 85 BsmI GAATG_Cn' Cuts at: 2645 3089 3317 3410 2645 Size: 444 228 93 5041 Fragments arranged by size: 5041 444 228 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2042 2089 2943 3125 3867 3952 Size: 399 241 11 47 854 182 742 85 Cuts at: 3952 5023 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 854 742 399 241 182 85 47 11 BspEI T'CCGG_A Cuts at: 5469 5469 Size: 5806 BspHI T'CATG_A Cuts at: 2763 3695 5425 2763 Size: 932 1730 3144 Fragments arranged by size: 3144 1730 932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3161 844 Size: 2317 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2133 3693 1394 Size: 429 310 1560 3507 Fragments arranged by size: 3507 1560 429 310 BsrDI GCAATG_nn' Cuts at: 65 1248 4301 4475 65 Size: 1183 3053 174 1396 Fragments arranged by size: 3053 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2468 4450 4637 1700 Size: 110 658 1982 187 2869 Fragments arranged by size: 2869 1982 658 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 2195 2249 1925 Size: 270 54 5482 Fragments arranged by size: 5482 270 54 BssSI C'ACGA_G Cuts at: 3866 4878 3866 Size: 1012 4794 BstAPI GCAn_nnn'nTGC Cuts at: 794 2979 794 Size: 2185 3621 BstBI TT'CG_AA Cuts at: 4610 4610 Size: 5806 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5806 BstYI r'GATC_y Cuts at: 1972 3006 3136 3503 3888 3905 5357 5443 5455 Size: 1034 130 367 385 17 1452 86 12 Cuts at: 5455 5801 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1034 385 367 346 130 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2878 2878 Size: 5806 BtgI C'CryG_G Cuts at: 513 1065 2238 513 Size: 552 1173 4081 Fragments arranged by size: 4081 1173 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5067 Fragments arranged by size: 5067 683 56 BtsI GCAGTG_nn' Cuts at: 2617 3322 4116 4136 2617 Size: 705 794 20 4287 Fragments arranged by size: 4287 794 705 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2241 2286 Size: 54 1425 50 275 18 354 57 45 Cuts at: 2286 3253 4142 5771 8 Size: 967 889 1629 43 Fragments arranged by size: 1629 1425 967 889 354 275 57 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2241 3253 1537 Size: 275 429 1012 4090 Fragments arranged by size: 4090 1012 429 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2909 3024 3736 1353 Size: 1556 115 712 3423 Fragments arranged by size: 3423 1556 712 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2391 2410 2428 2907 4393 4767 Size: 1064 138 802 19 18 479 1486 374 Cuts at: 4767 4913 387 Size: 146 1280 Fragments arranged by size: 1486 1280 1064 802 479 374 146 138 19 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2691 2733 3105 3869 4466 5253 5521 Size: 393 1059 42 372 764 597 787 268 Cuts at: 5521 1239 Size: 1524 Fragments arranged by size: 1524 1059 787 764 597 393 372 268 42 EcoNI CCTnn'n_nnAGG Cuts at: 2947 2947 Size: 5806 EcoO109I rG'GnC_Cy Cuts at: 2047 2047 Size: 5806 EcoRI G'AATT_C Cuts at: 3115 3493 3115 Size: 378 5428 FspI TGC'GCA Cuts at: 4312 4312 Size: 5806 HaeII r_GCGC'y Cuts at: 1678 2128 2172 2229 2667 3084 4953 1678 Size: 450 44 57 438 417 1869 2531 Fragments arranged by size: 2531 1869 450 438 417 57 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2671 2703 2991 Size: 32 135 32 524 32 543 32 288 Cuts at: 2991 3023 4447 4479 4521 4553 1373 Size: 32 1424 32 42 32 2626 Fragments arranged by size: 2626 1424 543 524 288 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3150 3396 669 Size: 988 281 1212 246 3079 Fragments arranged by size: 3079 1212 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5806 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KasI G'GCGC_C Cuts at: 2168 2168 Size: 5806 KpnI G_GTAC'C Cuts at: 3131 3131 Size: 5806 MfeI C'AATT_G Cuts at: 3405 3405 Size: 5806 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4920 5104 1305 Size: 3615 184 2007 Fragments arranged by size: 3615 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3984 Fragments arranged by size: 3984 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2258 2309 2762 3094 3764 4123 4282 Size: 648 1092 51 453 332 670 359 159 Cuts at: 4282 518 Size: 2042 Fragments arranged by size: 2042 1092 670 648 453 359 332 159 51 MspA1I CmG'CkG Cuts at: 1606 2240 2325 3222 3899 5047 5292 1606 Size: 634 85 897 677 1148 245 2120 Fragments arranged by size: 2120 1148 897 677 634 245 85 NaeI GCC'GGC Cuts at: 2470 2470 Size: 5806 NarI GG'CG_CC Cuts at: 2169 2169 Size: 5806 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5254 NdeI CA'TA_TG Cuts at: 387 387 Size: 5806 NgoMIV G'CCGG_C Cuts at: 2468 2468 Size: 5806 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 2241 3253 2241 Size: 1012 4794 NsiI A_TGCA'T Cuts at: 2645 2645 Size: 5806 NspI r_CATG'y Cuts at: 1132 1229 2263 2515 3164 4709 1132 Size: 97 1034 252 649 1545 2229 Fragments arranged by size: 2229 1545 1034 649 252 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 4705 4705 Size: 5806 PflMI CCAn_nnn'nTGG Cuts at: 1108 2316 2539 1108 Size: 1208 223 4375 Fragments arranged by size: 4375 1208 223 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 3376 3376 Size: 5806 PstI C_TGCA'G Cuts at: 830 2624 2975 3158 830 Size: 1794 351 183 3478 Fragments arranged by size: 3478 1794 351 183 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 SacII CC_GC'GG Cuts at: 2241 2241 Size: 5806 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SapI GCTCTTCn'nnn_ Cuts at: 3024 3024 Size: 5806 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 4054 5766 4054 Size: 1712 4094 SfcI C'TryA_G Cuts at: 826 1045 2620 2971 3154 4289 4970 5161 826 Size: 219 1575 351 183 1135 681 191 1471 Fragments arranged by size: 1575 1471 1135 681 351 219 191 183 SfoI GGC'GCC Cuts at: 2170 2170 Size: 5806 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 2468 1700 Size: 768 5038 SmaI CCC'GGG Cuts at: 1872 2032 2052 3133 1872 Size: 160 20 1081 4545 Fragments arranged by size: 4545 1081 160 20 SmlI C'TyrA_G Cuts at: 820 1017 1943 3910 4811 5073 820 Size: 197 926 1967 901 262 1553 Fragments arranged by size: 1967 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3597 493 Size: 3104 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 2515 3164 1132 Size: 1383 649 3774 Fragments arranged by size: 3774 1383 649 SrfI GCCC'GGGC Cuts at: 2032 2032 Size: 5806 SspI AAT'ATT Cuts at: 5 52 3730 5 Size: 47 3678 2081 Fragments arranged by size: 3678 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5806 StyI C'CwwG_G Cuts at: 513 1065 2061 3245 513 Size: 552 996 1184 3074 Fragments arranged by size: 3074 1184 996 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3833 3850 4003 4188 4633 325 Size: 1255 2253 17 153 185 445 1498 Fragments arranged by size: 2253 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4052 5764 96 Size: 274 80 33 51 1443 2075 1712 138 Fragments arranged by size: 2075 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2911 2943 5766 5798 2911 Size: 32 2823 32 2919 Fragments arranged by size: 2919 2823 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2020 2020 Size: 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 2030 2050 3131 1870 Size: 160 20 1081 4545 Fragments arranged by size: 4545 1081 160 20 XmnI GAAnn'nnTTC Cuts at: 3935 3935 Size: 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SwaI