################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MseI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 1009 GTCTAC GT^[AC][GT]AC AcsI 1326 GAATTC [GA]^AATT[CT] AflIII 413 ACACGT A^C[GA][CT]GT ApoI 1326 GAATTC [GA]^AATT[CT] BanI 460 GGCGCC G^G[CT][GA]CC BbeI 460 GGCGCC GGCGC^C BcnI 89 CCGGG CC^[GC]GG BsaI 1295 GAGACC GGTCTC(1/5) BspMI 1005 GCAGGT ACCTGC(4/8) BsrFI 1184 ACCGGC [GA]^CCGG[CT] BstXI 533 CCATTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1184 ACCGGC [GA]^CCGG[CT] DdeI 650 CTCAG C^T[ACGT]AG DraII 717 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 717 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 1326 GAATTC G^AATTC EcoT14I 398 CCATGG C^C[AT][AT]GG FauI 193 CCCGC CCCGC(4/6) HaeII 460 GGCGCC [GA]GCGC^[CT] KasI 460 GGCGCC G^GCGCC MaeII 415 ACGT A^CGT MboII 1275 GAAGA GAAGA(8/7) MspA1I 500 CAGCTG C[AC]G^C[GT]G NarI 460 GGCGCC GG^CGCC NciI 89 CCGGG CC^[GC]GG NcoI 398 CCATGG C^CATGG PleI 1219 GAGTC GAGTC(4/5) PpuMI 717 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 500 CAGCTG CAG^CTG SfcI 590 CTACAG C^T[GA][CT]AG SfoI 460 GGCGCC GGC^GCC SmlI 1159 CTCAAG C^T[CT][GA]AG SspI 1388 AATATT AAT^ATT StyI 398 CCATGG C^C[AT][AT]GG TaqI 1089 TCGA T^CGA TfiI 624 GATTC G^A[AT]TC TthHB8I 1089 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AlwI 565 GATCC GGATC(4/5) 323 GGATC AspHI 1038 GAGCTC G[AT]GC[AT]^C 1156 GAGCTC BalI 2 TGGCCA TGG^CCA 709 TGGCCA BanII 1038 GAGCTC G[GA]GC[CT]^C 1156 GAGCTC BmyI 1038 GAGCTC G[AGT]GC[ACT]^C 1156 GAGCTC BsgI 53 GTGCAG GTGCAG(16/14) 235 GTGCAG BsiHKAI 1038 GAGCTC G[AT]GC[AT]^C 1156 GAGCTC BsiYI 203 CCCAGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 204 CCAGCCCTGGG BslI 203 CCCAGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 204 CCAGCCCTGGG BsmAI 112 GAGAC GTCTC(1/5) 1295 GAGAC Bsp1286I 1038 GAGCTC G[AGT]GC[ACT]^C 1156 GAGCTC Bsp1407I 1049 TGTACA T^GTACA 1199 TGTACA BspHI 861 TCATGA T^CATGA 914 TCATGA BsrGI 1049 TGTACA T^GTACA 1199 TGTACA DpnII 324 GATC ^GATC 565 GATC DsaI 98 CCACGG C^C[GA][CT]GG 398 CCATGG HapII 89 CCGG C^CGG 1185 CCGG HgaI 58 GACGC GACGC(5/10) 391 GACGC HinfI 624 GATTC G^A[ACGT]TC 1219 GAGTC HpaII 89 CCGG C^CGG 1185 CCGG MboI 324 GATC ^GATC 565 GATC MluNI 2 TGGCCA TGG^CCA 709 TGGCCA MscI 2 TGGCCA TGG^CCA 709 TGGCCA MslI 498 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 534 CATTGCGGTG MspI 89 CCGG C^CGG 1185 CCGG NdeII 324 GATC ^GATC 565 GATC NheI 81 GCTAGC G^CTAGC 1018 GCTAGC RcaI 861 TCATGA T^CATGA 914 TCATGA SacI 1038 GAGCTC GAGCT^C 1156 GAGCTC Sau3AI 324 GATC ^GATC 565 GATC SexAI 1168 ACCAGGT A^CC[AT]GGT 1261 ACCAGGT SspBI 1049 TGTACA T^GTACA 1199 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaII BbvI BfaI BglI BsaHI BsaJI BseRI BsmFI BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I EaeI EcoRII Fnu4HI FokI HaeIII HhaI Hin1I HinP1I HphI ItaI MaeI MaeIII MnlI MvaI MvnI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI