(Circular) (Six-base) MAPSORT of: pFN21AB5877.seq Check: 6624 from: 1 to: 6100 pFN21AB5877 6100 bp With 182 enzymes: SgfI * March 28, 2012 12:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3015 3443 1937 Size: 1078 428 4594 Fragments arranged by size: 4594 1078 428 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4163 5547 5815 1632 Size: 2531 1384 268 1917 Fragments arranged by size: 2531 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 2418 4999 5976 1203 Size: 1215 2581 977 1327 Fragments arranged by size: 2581 1327 1215 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 4829 4829 Size: 6100 AleI CACnn'nnGTG Cuts at: 1166 2507 1166 Size: 1341 4759 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6100 AlwNI CAG_nnn'CTG Cuts at: 3843 5415 3843 Size: 1572 4528 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 3331 3409 3635 3787 5896 1493 Size: 1838 78 226 152 2109 1697 Fragments arranged by size: 2109 1838 1697 226 152 78 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 3425 1870 Size: 73 1482 4545 Fragments arranged by size: 4545 1482 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2465 3421 4876 618 Size: 325 217 627 678 956 1455 1842 Fragments arranged by size: 1842 1455 956 678 627 325 217 BanII G_rGCy'C Cuts at: 1391 3047 3165 3419 1391 Size: 1656 118 254 4072 Fragments arranged by size: 4072 1656 254 118 BbeI G_GCGC'C Cuts at: 2469 2469 Size: 6100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4291 4325 1903 Size: 34 2354 34 3678 Fragments arranged by size: 3678 2354 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2099 2108 2251 Size: 107 122 71 631 605 427 9 143 Cuts at: 2251 2405 4711 136 Size: 154 2306 1525 Fragments arranged by size: 2306 1525 631 605 427 154 143 122 107 71 9 BglII A'GATC_T Cuts at: 6095 6095 Size: 6100 BlpI GC'TnA_GC Cuts at: 3517 3517 Size: 6100 Bme1580I G_kGCm'C Cuts at: 1790 4161 5317 1790 Size: 2371 1156 2573 Fragments arranged by size: 2573 2371 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2130 3831 4789 Size: 416 53 83 399 24 713 1701 958 Cuts at: 4789 442 Size: 1753 Fragments arranged by size: 1753 1701 958 713 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2090 3027 1056 Size: 1034 937 4129 Fragments arranged by size: 4129 1034 937 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4760 1239 Size: 3521 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3149 4225 5090 5388 3149 Size: 1076 865 298 3861 Fragments arranged by size: 3861 1076 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3294 4763 882 Size: 617 1795 1469 2219 Fragments arranged by size: 2219 1795 1469 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2397 2466 3907 Size: 53 83 186 936 531 333 69 1441 Cuts at: 3907 4289 4924 275 Size: 382 635 1451 Fragments arranged by size: 1451 1441 936 635 531 382 333 186 83 69 53 BsaWI w'CCGG_w Cuts at: 4533 4931 5205 5352 5763 4533 Size: 398 274 147 411 4870 Fragments arranged by size: 4870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 1178 Size: 30 165 30 741 30 5104 Fragments arranged by size: 5104 741 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2149 2152 2847 1186 Size: 963 3 695 4439 Fragments arranged by size: 4439 963 695 3 BseYI C'CCAG_C Cuts at: 2017 2176 2208 5303 5799 2017 Size: 159 32 3095 496 2318 Fragments arranged by size: 3095 2318 496 159 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2079 2261 1163 Size: 573 343 182 5002 Fragments arranged by size: 5002 573 343 182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3047 3165 4161 4246 5317 3047 Size: 118 996 85 1071 3830 Fragments arranged by size: 3830 1071 996 118 85 BsmI GAATG_Cn' Cuts at: 3611 3704 3611 Size: 93 6007 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3047 3165 3419 4161 4246 5317 1391 Size: 399 1257 118 254 742 85 1071 2174 Fragments arranged by size: 2174 1257 1071 742 399 254 118 85 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 2866 2919 3989 5719 2866 Size: 53 1070 1730 3247 Fragments arranged by size: 3247 1730 1070 53 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3001 3455 844 Size: 2157 454 3489 Fragments arranged by size: 3489 2157 454 BsrBI CCG'CTC Cuts at: 1394 1823 3987 1394 Size: 429 2164 3507 Fragments arranged by size: 3507 2164 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2538 2717 2780 3045 4595 4769 65 Size: 1183 1290 179 63 265 1550 174 1396 Fragments arranged by size: 1550 1396 1290 1183 265 179 174 63 BsrFI r'CCGG_y Cuts at: 1700 1810 3189 4744 4931 1700 Size: 110 1379 1555 187 2869 Fragments arranged by size: 2869 1555 1379 187 110 BsrGI T'GTAC_A Cuts at: 96 3054 3204 96 Size: 2958 150 2992 Fragments arranged by size: 2992 2958 150 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6100 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstXI CCAn_nnnn'nTGG Cuts at: 2545 2545 Size: 6100 BstYI r'GATC_y Cuts at: 1972 3430 3797 4182 4199 5651 5737 5749 6095 Size: 1458 367 385 17 1452 86 12 346 Cuts at: 6095 1972 Size: 1977 Fragments arranged by size: 1977 1458 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2103 2403 513 Size: 552 939 99 300 4210 Fragments arranged by size: 4210 939 552 300 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2242 487 Size: 683 56 1016 4345 Fragments arranged by size: 4345 1016 683 56 BtsI GCAGTG_nn' Cuts at: 2301 2967 3616 4410 4430 2301 Size: 666 649 794 20 3971 Fragments arranged by size: 3971 794 666 649 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2100 2406 Size: 54 1425 50 275 18 177 93 306 Cuts at: 2406 2535 2714 3547 4436 6065 8 Size: 129 179 833 889 1629 43 Fragments arranged by size: 1629 1425 889 833 306 275 179 177 129 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4030 1353 Size: 2677 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3337 4687 5061 5207 387 Size: 1064 138 1748 1350 374 146 1280 Fragments arranged by size: 1748 1350 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3045 3163 3045 Size: 118 5982 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4163 4760 5547 5815 1239 Size: 393 2531 597 787 268 1524 Fragments arranged by size: 2531 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2723 2723 Size: 6100 EcoRI G'AATT_C Cuts at: 3331 3409 3787 3331 Size: 78 378 5644 Fragments arranged by size: 5644 378 78 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 2469 5247 1678 Size: 791 2778 2531 Fragments arranged by size: 2778 2531 791 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2823 2855 4741 4773 4815 Size: 32 135 32 1251 32 1886 32 42 Cuts at: 4815 4847 1373 Size: 32 2626 Fragments arranged by size: 2626 1886 1251 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3444 3690 669 Size: 988 281 1506 246 3079 Fragments arranged by size: 3079 1506 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KasI G'GCGC_C Cuts at: 2465 2465 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2418 2794 3257 5214 5398 1305 Size: 1113 376 463 1957 184 2007 Fragments arranged by size: 2007 1957 1113 463 376 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2716 10 Size: 54 1425 343 177 707 3394 Fragments arranged by size: 3394 1425 707 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2507 2543 4058 4417 4576 518 Size: 648 1341 36 1515 359 159 2042 Fragments arranged by size: 2042 1515 1341 648 359 159 36 MspA1I CmG'CkG Cuts at: 1606 2507 3516 4193 5341 5586 1606 Size: 901 1009 677 1148 245 2120 Fragments arranged by size: 2120 1148 1009 901 677 245 NarI GG'CG_CC Cuts at: 2466 2466 Size: 6100 NcoI C'CATG_G Cuts at: 513 1065 2004 2403 513 Size: 552 939 399 4210 Fragments arranged by size: 4210 939 552 399 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NheI G'CTAG_C Cuts at: 1052 2086 3023 1052 Size: 1034 937 4129 Fragments arranged by size: 4129 1034 937 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 3458 5003 1132 Size: 97 2229 1545 2229 Fragments arranged by size: 2229 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2214 1348 Size: 866 5234 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6100 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2723 2723 Size: 6100 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PstI C_TGCA'G Cuts at: 830 3452 830 Size: 2622 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 2507 2507 Size: 6100 SacI G_AGCT'C Cuts at: 3047 3165 3047 Size: 118 5982 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SexAI A'CCwGG_T Cuts at: 3173 3266 3173 Size: 93 6007 SfcI C'TryA_G Cuts at: 826 1045 2595 3448 4583 5264 5455 826 Size: 219 1550 853 1135 681 191 1471 Fragments arranged by size: 1550 1471 1135 853 681 219 191 SfoI GGC'GCC Cuts at: 2467 2467 Size: 6100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 3427 1872 Size: 1555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3164 4204 5105 5367 820 Size: 197 926 1221 1040 901 262 1553 Fragments arranged by size: 1553 1221 1040 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SspI AAT'ATT Cuts at: 5 52 3395 4024 5 Size: 47 3343 629 2081 Fragments arranged by size: 3343 2081 629 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6100 StyI C'CwwG_G Cuts at: 513 1065 2004 2403 3539 513 Size: 552 939 399 1136 3074 Fragments arranged by size: 3074 1136 939 552 399 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 2112 4127 4144 4297 4482 4927 Size: 1255 465 67 2015 17 153 185 445 Cuts at: 4927 325 Size: 1498 Fragments arranged by size: 2015 1498 1255 465 445 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2769 3054 3204 Size: 274 80 33 51 1443 792 285 150 Cuts at: 3204 4346 6058 96 Size: 1142 1712 138 Fragments arranged by size: 1712 1443 1142 792 285 274 150 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2172 2204 6060 6092 2172 Size: 32 3856 32 2180 Fragments arranged by size: 3856 2180 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 3425 1870 Size: 1555 4545 XmnI GAAnn'nnTTC Cuts at: 4229 4229 Size: 6100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI