################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AlwI 658 GATCC GGATC(4/5) Asp700I 4 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 754 GAGCAC G[AT]GC[AT]^C BclI 684 TGATCA T^GATCA BcnI 661 CCGGG CC^[GC]GG BsgI 761 CTGCAC GTGCAG(16/14) BsiHKAI 754 GAGCAC G[AT]GC[AT]^C BspHI 741 TCATGA T^CATGA BstNI 412 CCAGG CC^[AT]GG BstYI 657 AGATCC [GA]^GATC[CT] Cac8I 535 GCAAGC GC[ACGT]^[ACGT]GC EcoRII 412 CCAGG ^CC[AT]GG EcoT22I 434 ATGCAT ATGCA^T FbaI 684 TGATCA T^GATCA HincII 225 GTCAAC GT[CT]^[GA]AC HindII 225 GTCAAC GT[CT]^[GA]AC HindIII 715 AAGCTT A^AGCTT MaeII 311 ACGT A^CGT MflI 657 AGATCC [GA]^GATC[CT] MvaI 412 CCAGG CC^[AT]GG MwoI 50 GCAGTAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 661 CCGGG CC^[GC]GG NlaIII 742 CATG CATG^ NsiI 434 ATGCAT ATGCA^T RcaI 741 TCATGA T^CATGA SexAI 411 ACCAGGT A^CC[AT]GGT SmlI 503 CTCAAG C^T[CT][GA]AG TfiI 136 GAATC G^A[AT]TC XhoII 657 AGATCC [GA]^GATC[CT] XmnI 4 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 455 GTAC GT^AC 636 GTAC BbvI 47 GCAGC GCAGC(8/12) 76 GCAGC BmyI 663 GGGCAC G[AGT]GC[ACT]^C 754 GAGCAC BsiYI 163 CCAACAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 670 CCAGAAAATGG BslI 163 CCAACAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 670 CCAGAAAATGG BsmAI 216 GTCTC GTCTC(1/5) 501 GTCTC Bsp1286I 663 GGGCAC G[AGT]GC[ACT]^C 754 GAGCAC BsrFI 554 GCCGGT [GA]^CCGG[CT] 579 GCCGGT BssKI 412 CCAGG ^CC[ACGT]GG 661 CCGGG Cfr10I 554 GCCGGT [GA]^CCGG[CT] 579 GCCGGT DpnII 658 GATC ^GATC 685 GATC Fnu4HI 47 GCAGC GC^[ACGT]GC 76 GCAGC ItaI 47 GCAGC GC^[ACGT]GC 76 GCAGC MboI 658 GATC ^GATC 685 GATC MboII 288 GAAGA GAAGA(8/7) 30 TCTTC NdeII 658 GATC ^GATC 685 GATC PflMI 163 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 670 CCAGAAAATGG PleI 295 GACTC GAGTC(4/5) 745 GAGTC RsaI 455 GTAC GT^AC 636 GTAC Sau3AI 658 GATC ^GATC 685 GATC ScrFI 412 CCAGG CC^[ACGT]GG 661 CCGGG SfaNI 433 GATGC GCATC(5/9) 436 GCATC TseI 47 GCAGC G^C[AT]GC 76 GCAGC Tsp45I 16 GTGAC ^GT[GC]AC 157 GTCAC TspRI 276 CACTG CA[GC]TG[ACGT][ACGT]^ 516 CACTG Van91I 163 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 670 CCAGAAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AluI BsrI DdeI HapII HinfI HpaII HphI MaeIII MnlI MseI MspI Tru9I Tsp509I