Restriction map for
pFN21AE2253
(Circular) (Six-base) MAPSORT of: pFN21AE2253.seq Check: 5915 from: 1 to: 5488
pFN21AE2253 5488 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 2787 3615 3988 1563
Size: 1224 828 373 3063
Fragments arranged by size:
3063 1224 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2532 3551 4935 5203 1632
Size: 900 1019 1384 268 1917
Fragments arranged by size:
1917 1384 1019 900 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 3231 4803 3231
Size: 1572 3916
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2813 1870
Size: 73 870 4545
Fragments arranged by size:
4545 870 73
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2809 4264 618
Size: 325 217 627 1022 1455 1842
Fragments arranged by size:
1842 1455 1022 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2807 1391
Size: 1416 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 2689 1548
Size: 1141 4347
BfrBI ATG'CAT
Cuts at: 2441 2441
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4099 136
Size: 107 122 71 631 605 2427 1525
Fragments arranged by size:
2427 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 2672 3549 4705 1790
Size: 882 877 1156 2573
Fragments arranged by size:
2573 1156 882 877
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2037 3219 4177
Size: 416 53 83 399 24 620 1182 958
Cuts at: 4177 442
Size: 1753
Fragments arranged by size:
1753 1182 958 620 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4148 1239
Size: 2909 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2493 3613 4478 4776 2493
Size: 1120 865 298 3205
Fragments arranged by size:
3205 1120 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4151 882
Size: 617 2652 2219
Fragments arranged by size:
2652 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3279 493
Size: 711 2075 2702
Fragments arranged by size:
2702 2075 711
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3295 3677 4312 275
Size: 53 83 186 936 1762 382 635 1451
Fragments arranged by size:
1762 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3921 4319 4593 4740 5151 3921
Size: 398 274 147 411 4258
Fragments arranged by size:
4258 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5263
Fragments arranged by size:
5263 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5488
BseYI C'CCAG_C
Cuts at: 4691 5187 4691
Size: 496 4992
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2751 1163
Size: 573 1015 3900
Fragments arranged by size:
3900 1015 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2938 3699 4727 1540
Size: 275 186 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2763 3549 3634 4705 2763
Size: 786 85 1071 3546
Fragments arranged by size:
3546 1071 786 85
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2672 2763 2807 3549 3634 4705 1391
Size: 399 882 91 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 882 742 399 91 85 44
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 2746 3377 5107 2746
Size: 631 1730 3127
Fragments arranged by size:
3127 1730 631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2843 844
Size: 1999 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 1394
Size: 429 1552 3507
Fragments arranged by size:
3507 1552 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3983 4157 65
Size: 1183 2735 174 1396
Fragments arranged by size:
2735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2559 2584 4132 4319 1700
Size: 110 749 25 1548 187 2869
Fragments arranged by size:
2869 1548 749 187 110 25
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 3548 4560 3548
Size: 1012 4476
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstYI r'GATC_y
Cuts at: 1972 2662 2818 3185 3570 3587 5039 5125 5137
Size: 690 156 367 385 17 1452 86 12
Cuts at: 5137 5483 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 690 385 367 346 156 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 3004 3798 3818 3004
Size: 794 20 4674
Fragments arranged by size:
4674 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2935 3824 5453
Size: 54 1425 50 275 18 1105 889 1629
Cuts at: 5453 8
Size: 43
Fragments arranged by size:
1629 1425 1105 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2935 1537
Size: 275 1123 4090
Fragments arranged by size:
4090 1123 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3418 1353
Size: 2065 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4075 4449 4595 387
Size: 1064 138 2486 374 146 1280
Fragments arranged by size:
2486 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 3551 4148 4935 5203 1239
Size: 393 900 1019 597 787 268 1524
Fragments arranged by size:
1524 1019 900 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 4635 1678
Size: 2957 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2371 2403 2728 2760 4129
Size: 32 135 32 799 32 325 32 1369
Cuts at: 4129 4161 4203 4235 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1369 799 325 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2232 2832 3078 669
Size: 988 281 294 600 246 3079
Fragments arranged by size:
3079 988 600 294 281 246
HindIII A'AGCT_T
Cuts at: 748 2720 748
Size: 1972 3516
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3666
Fragments arranged by size:
3666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3446 3805 3964 518
Size: 648 2280 359 159 2042
Fragments arranged by size:
2280 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2904 3581 4729 4974 1606
Size: 1298 677 1148 245 2120
Fragments arranged by size:
2120 1298 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NsiI A_TGCA'T
Cuts at: 2443 2443
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2846 4391 1132
Size: 97 1617 1545 2229
Fragments arranged by size:
2229 1617 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5488
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2174 2681 1108
Size: 1066 507 3915
Fragments arranged by size:
3915 1066 507
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2840 830
Size: 2010 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SexAI A'CCwGG_T
Cuts at: 2416 2416
Size: 5488
SfcI C'TryA_G
Cuts at: 826 1045 2836 3971 4652 4843 826
Size: 219 1791 1135 681 191 1471
Fragments arranged by size:
1791 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2508 3592 4493 4755 820
Size: 197 926 565 1084 901 262 1553
Fragments arranged by size:
1553 1084 926 901 565 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5488
StyI C'CwwG_G
Cuts at: 513 1065 2004 2927 513
Size: 552 939 923 3074
Fragments arranged by size:
3074 939 923 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3515 3532 3685 3870 4315 325
Size: 1255 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3734 5446 96
Size: 274 80 33 51 1443 1757 1712 138
Fragments arranged by size:
1757 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5448 5480 5448
Size: 32 5456
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5488
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 2013 3617 2013
Size: 1604 3884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI