(Linear) (Six-base) MAPSORT of: KIEE2253.seq  Check: 8500  from: 1  to: 783



KIEE2253.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     783 
   Size:        527     256
 
BclI T'GATC_A

Cuts at:      0     684     783 
   Size:        684      99
 
BfrBI ATG'CAT

Cuts at:      0     436     783 
   Size:        436     347
 
Bme1580I G_kGCm'C

Cuts at:      0     667     783 
   Size:        667     116
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      32     783 
   Size:         32     751
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488     783 
   Size:        488     295
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746     783 
   Size:        746      37
 
BsiHKAI G_wGCw'C

Cuts at:      0     758     783 
   Size:        758      25
 
Bsp1286I G_dGCh'C

Cuts at:      0     667     758     783 
   Size:        667      91      25

  Fragments arranged by size: 

                 667      91      25
 
BspHI T'CATG_A

Cuts at:      0     741     783 
   Size:        741      42
 
BsrFI r'CCGG_y

Cuts at:      0     554     579     783 
   Size:        554      25     204

  Fragments arranged by size: 

                 554     204      25
 
BstYI r'GATC_y

Cuts at:      0     657     783 
   Size:        657     126
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     783 
   Size:        527     256
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     366     398     723     755     783 
   Size:        366      32     325      32      28

  Fragments arranged by size: 

                 366     325      32      32      28
 
HincII GTy'rAC

Cuts at:      0     227     783 
   Size:        227     556
 
HindIII A'AGCT_T

Cuts at:      0     715     783 
   Size:        715      68
 
NsiI A_TGCA'T

Cuts at:      0     438     783 
   Size:        438     345
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     169     676     783 
   Size:        169     507     107

  Fragments arranged by size: 

                 507     169     107
 
SexAI A'CCwGG_T

Cuts at:      0     411     783 
   Size:        411     372
 
SmlI C'TyrA_G

Cuts at:      0     503     783 
   Size:        503     280
 
XmnI GAAnn'nnTTC

Cuts at:      0       8     783 
   Size:          8     775
 

 Enzymes that do cut:

     AcuI     BclI    BfrBI Bme1580I     BmrI    BpuEI     BsgI  BsiHKAI
 Bsp1286I    BspHI    BsrFI    BstYI  Eco57MI    Hin4I   HincII  HindIII
     NsiI    PflMI    SexAI     SmlI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI