Restriction map for
pFN21AE3133
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FauI Fnu4HI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HindIII HpaI HpaII
ItaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsaI RsrII
SacI SacII SalI Sau96I SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TseI Tsp45I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 180 CCGC CCGC(-3/-1)
AcsI 705 AAATTT [GA]^AATT[CT]
AflIII 270 ACGTGT A^C[GA][CT]GT
AhdI 871 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 901 GGATC GGATC(4/5)
ApoI 705 AAATTT [GA]^AATT[CT]
Asp700I 757 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 871 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 514 GAGCAC G[AT]GC[AT]^C
AspI 227 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 541 GAAGAC GAAGAC(2/6)
BclI 327 TGATCA T^GATCA
BfaI 946 CTAG C^TAG
BglI 434 GCCACGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 541 GAAGAC GAAGAC(2/6)
BsaJI 435 CCACGG C^C[ACGT][ACGT]GG
BsgI 415 GTGCAG GTGCAG(16/14)
BsiHKAI 514 GAGCAC G[AT]GC[AT]^C
BsmAI 785 GAGAC GTCTC(1/5)
BsmFI 645 GGGAC GGGAC(10/14)
BspHI 867 TCATGA T^CATGA
BssKI 774 CCTGG ^CC[ACGT]GG
BstAPI 408 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 275 TTCGAA TT^CGAA
BstNI 774 CCTGG CC^[AT]GG
DdeI 249 CTCAG C^T[ACGT]AG
DsaI 435 CCACGG C^C[GA][CT]GG
Eam1105I 871 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 774 CCTGG ^CC[AT]GG
EcoRV 496 GATATC GAT^ATC
FbaI 327 TGATCA T^GATCA
HgaI 664 GCGTC GACGC(5/10)
MaeI 946 CTAG C^TAG
MaeIII 383 GTTAC ^GT[ACGT]AC
MslI 517 CACTATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 445 CAGCTG C[AC]G^C[GT]G
MvaI 774 CCTGG CC^[AT]GG
NlaIV 240 GGAACC GG[ACGT]^[ACGT]CC
NspV 275 TTCGAA TT^CGAA
PflFI 227 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 647 GACTC GAGTC(4/5)
PvuII 445 CAGCTG CAG^CTG
RcaI 867 TCATGA T^CATGA
SapI 163 GAAGAGC GCTCTTC(1/4)
ScrFI 774 CCTGG CC^[ACGT]GG
SfaNI 408 GCATC GCATC(5/9)
SfuI 275 TTCGAA TT^CGAA
StuI 602 AGGCCT AGG^CCT
Tth111I 227 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 757 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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BmyI 401 GGGCAC G[AGT]GC[ACT]^C
514 GAGCAC
BseRI 76 GAGGAG GAGGAG(10/8)
922 GAGGAG
BsiYI 91 CCAAGATTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
636 CCATCACAAGG
BslI 91 CCAAGATTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
636 CCATCACAAGG
Bsp1286I 401 GGGCAC G[AGT]GC[ACT]^C
514 GAGCAC
Cac8I 18 GCCAGC GC[ACGT]^[ACGT]GC
443 GCCAGC
EarI 163 GAAGAG CTCTTC(1/4)
278 GAAGAG
HaeIII 442 GGCC GG^CC
603 GGCC
HincII 43 GTTGAC GT[CT]^[GA]AC
484 GTCAAC
HindII 43 GTTGAC GT[CT]^[GA]AC
484 GTCAAC
Ksp632I 163 GAAGAG CTCTTC(1/4)
278 GAAGAG
MaeII 270 ACGT A^CGT
559 ACGT
TaqI 276 TCGA T^CGA
726 TCGA
TthHB8I 276 TCGA T^CGA
726 TCGA
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BsrI BstF5I DpnII FokI
HinfI HphI MboI MboII MnlI
MseI MwoI NdeII NlaIII Sau3AI
SfcI TfiI Tru9I Tsp509I TspRI