(Circular) (Six-base) MAPSORT of: pFN21AE3133.seq Check: 5083 from: 1 to: 5722 pFN21AE3133 5722 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3065 1937 Size: 1128 4594 Acc65I G'GTAC_C Cuts at: 3043 3043 Size: 5722 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2606 3785 5169 5437 1632 Size: 974 1179 1384 268 1917 Fragments arranged by size: 1917 1384 1179 974 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 2275 3019 4621 5598 1203 Size: 1072 744 1602 977 1327 Fragments arranged by size: 1602 1327 1072 977 744 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 2881 4451 2881 Size: 1570 4152 AleI CACnn'nnGTG Cuts at: 1166 2526 1166 Size: 1360 4362 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5722 AlwNI CAG_nnn'CTG Cuts at: 3465 5037 3465 Size: 1572 4150 ApaLI G'TGCA_C Cuts at: 3779 4935 3779 Size: 1156 4566 ApoI r'AATT_y Cuts at: 1493 2710 3031 3257 3409 5518 1493 Size: 1217 321 226 152 2109 1697 Fragments arranged by size: 2109 1697 1217 321 226 152 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 3047 1870 Size: 73 1104 4545 Fragments arranged by size: 4545 1104 73 BamHI G'GATC_C Cuts at: 3052 3419 3052 Size: 367 5355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3043 4498 618 Size: 325 217 627 1256 1455 1842 Fragments arranged by size: 1842 1455 1256 627 325 217 BanII G_rGCy'C Cuts at: 1391 3041 1391 Size: 1650 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2553 928 Size: 971 654 4097 Fragments arranged by size: 4097 971 654 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3913 3947 1903 Size: 34 1976 34 3678 Fragments arranged by size: 3678 1976 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3614 4824 3614 Size: 1210 4512 BclI T'GATC_A Cuts at: 1548 2332 1548 Size: 784 4938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2445 4333 136 Size: 107 122 71 631 605 773 1888 1525 Fragments arranged by size: 1888 1525 773 631 605 122 107 71 BglII A'GATC_T Cuts at: 5717 5717 Size: 5722 BlpI GC'TnA_GC Cuts at: 3139 3139 Size: 5722 Bme1580I G_kGCm'C Cuts at: 1790 2410 3783 4939 1790 Size: 620 1373 1156 2573 Fragments arranged by size: 2573 1373 1156 620 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5722 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3453 4411 442 Size: 416 53 83 399 24 2036 958 1753 Fragments arranged by size: 2036 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5722 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4382 1239 Size: 3143 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3847 4712 5010 3847 Size: 865 298 4559 Fragments arranged by size: 4559 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4385 882 Size: 617 2886 2219 Fragments arranged by size: 2886 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 5716 5716 Size: 5722 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3529 3911 4546 275 Size: 53 83 186 936 1996 382 635 1451 Fragments arranged by size: 1996 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4155 4553 4827 4974 5385 4155 Size: 398 274 147 411 4492 Fragments arranged by size: 4492 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2166 2196 2613 2643 1178 Size: 30 165 30 763 30 417 30 4257 Fragments arranged by size: 4257 763 417 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2942 1186 Size: 910 846 3966 Fragments arranged by size: 3966 910 846 BseYI C'CCAG_C Cuts at: 4925 5421 4925 Size: 496 5226 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 1163 Size: 573 705 4444 Fragments arranged by size: 4444 705 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3172 3933 4961 1540 Size: 275 186 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2523 3783 3868 4939 2523 Size: 1260 85 1071 3306 Fragments arranged by size: 3306 1260 1071 85 BsmI GAATG_Cn' Cuts at: 3233 3326 3233 Size: 93 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2410 2523 3041 3783 3868 4939 1391 Size: 399 620 113 518 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 620 518 399 113 85 BspEI T'CCGG_A Cuts at: 5385 5385 Size: 5722 BspHI T'CATG_A Cuts at: 2872 3611 5341 2872 Size: 739 1730 3253 Fragments arranged by size: 3253 1730 739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3077 844 Size: 2233 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3609 1394 Size: 429 1786 3507 Fragments arranged by size: 3507 1786 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4217 4391 65 Size: 1183 2969 174 1396 Fragments arranged by size: 2969 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4366 4553 1700 Size: 110 2556 187 2869 Fragments arranged by size: 2869 2556 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5722 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 3782 4794 3782 Size: 1012 4710 BstAPI GCAn_nnn'nTGC Cuts at: 794 2419 794 Size: 1625 4097 BstBI TT'CG_AA Cuts at: 2281 4526 2281 Size: 2245 3477 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstYI r'GATC_y Cuts at: 1972 3052 3419 3804 3821 5273 5359 5371 5717 Size: 1080 367 385 17 1452 86 12 346 Cuts at: 5717 1972 Size: 1977 Fragments arranged by size: 1977 1452 1080 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2440 513 Size: 552 1375 3795 Fragments arranged by size: 3795 1375 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4983 Fragments arranged by size: 4983 683 56 BtsI GCAGTG_nn' Cuts at: 2408 3238 4032 4052 2408 Size: 830 794 20 4078 Fragments arranged by size: 4078 830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5687 DraI TTT'AAA Cuts at: 3026 3026 Size: 5722 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4729 809 Size: 507 3413 1802 Fragments arranged by size: 3413 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3169 4058 5687 Size: 54 1425 50 275 18 1339 889 1629 Cuts at: 5687 8 Size: 43 Fragments arranged by size: 1629 1425 1339 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3169 1537 Size: 275 1357 4090 Fragments arranged by size: 4090 1357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2163 2278 3652 1353 Size: 810 115 1374 3423 Fragments arranged by size: 3423 1374 810 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4309 4683 4829 387 Size: 1064 138 2720 374 146 1280 Fragments arranged by size: 2720 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2606 3785 4382 5169 5437 1239 Size: 393 974 1179 597 787 268 1524 Fragments arranged by size: 1524 1179 974 787 597 393 268 EcoRI G'AATT_C Cuts at: 3031 3409 3031 Size: 378 5344 EcoRV GAT'ATC Cuts at: 2503 2503 Size: 5722 FspI TGC'GCA Cuts at: 4228 4228 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 4869 1678 Size: 3191 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2713 2745 2867 2899 4363 Size: 32 135 32 1141 32 122 32 1464 Cuts at: 4363 4395 4437 4469 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1464 1141 135 122 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2050 2491 3066 3312 669 Size: 988 281 112 441 575 246 3079 Fragments arranged by size: 3079 988 575 441 281 246 112 HindIII A'AGCT_T Cuts at: 748 748 Size: 5722 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KpnI G_GTAC'C Cuts at: 3047 3047 Size: 5722 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4836 5020 1305 Size: 3531 184 2007 Fragments arranged by size: 3531 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3900 Fragments arranged by size: 3900 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2526 3680 4039 4198 518 Size: 648 1360 1154 359 159 2042 Fragments arranged by size: 2042 1360 1154 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2452 3138 3815 4963 5208 1606 Size: 846 686 677 1148 245 2120 Fragments arranged by size: 2120 1148 846 686 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5170 NdeI CA'TA_TG Cuts at: 387 387 Size: 5722 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5722 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 3080 4625 1132 Size: 97 1851 1545 2229 Fragments arranged by size: 2229 1851 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5722 PciI A'CATG_T Cuts at: 4621 4621 Size: 5722 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5722 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2277 2309 3791 3823 5304 5336 2277 Size: 32 1482 32 1481 32 2663 Fragments arranged by size: 2663 1482 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PstI C_TGCA'G Cuts at: 830 3074 830 Size: 2244 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 PvuII CAG'CTG Cuts at: 2452 2452 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SapI GCTCTTCn'nnn_ Cuts at: 2163 2163 Size: 5722 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SfcI C'TryA_G Cuts at: 826 1045 2323 2524 3000 3070 4205 4886 5077 Size: 219 1278 201 476 70 1135 681 191 Cuts at: 5077 826 Size: 1471 Fragments arranged by size: 1471 1278 1135 681 476 219 201 191 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5722 SmaI CCC'GGG Cuts at: 1872 3049 1872 Size: 1177 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3826 4727 4989 820 Size: 197 926 1883 901 262 1553 Fragments arranged by size: 1883 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 3080 1132 Size: 1948 3774 SspI AAT'ATT Cuts at: 5 52 3646 5 Size: 47 3594 2081 Fragments arranged by size: 3594 2081 47 StuI AGG'CCT Cuts at: 1517 2609 1517 Size: 1092 4630 StyI C'CwwG_G Cuts at: 513 1065 3161 513 Size: 552 2096 3074 Fragments arranged by size: 3074 2096 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3749 3766 3919 4104 4549 325 Size: 1255 2169 17 153 185 445 1498 Fragments arranged by size: 2169 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3968 5680 96 Size: 274 80 33 51 1443 1991 1712 138 Fragments arranged by size: 1991 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2700 2732 5682 5714 2700 Size: 32 2950 32 2708 Fragments arranged by size: 2950 2708 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2235 1316 Size: 919 4803 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5722 XmaI C'CCGG_G Cuts at: 1870 3047 1870 Size: 1177 4545 XmnI GAAnn'nnTTC Cuts at: 2766 3851 2766 Size: 1085 4637 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI