##################################
# NO CUT ENZYMES (  211 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      ClaI        CpoI        
DdeI        DpnII       DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        HpaII       KasI        KpnI        
KspI        MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        ScrFI       SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AfaI          470  GTAC                 GT^AC
Asp700I        46  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI           88  GAAGAC               GAAGAC(2/6)
BbvI          354  GCTGC                GCAGC(8/12)
BfaI          193  CTAG                 C^TAG
BpuAI          88  GAAGAC               GAAGAC(2/6)
BsaAI         360  CACGTA               [CT]AC^GT[GA]
BsiYI         153  CCCCATTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          153  CCCCATTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BstXI         473  CCACACTTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          370  GCGC                 GCG^C
Cfr13I        581  GGCCC                G^G[ACGT]CC
DraII         580  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EarI          179  CTCTTC               CTCTTC(1/4)
EcoO109I      580  AGGCCCC              [GA]G^G[ACGT]CC[CT]
Fnu4HI        354  GCTGC                GC^[ACGT]GC
HhaI          370  GCGC                 GCG^C
HinP1I        370  GCGC                 G^CGC
ItaI          354  GCTGC                GC^[ACGT]GC
Ksp632I       179  CTCTTC               CTCTTC(1/4)
MaeI          193  CTAG                 C^TAG
MaeII         361  ACGT                 A^CGT
MwoI          547  GCCATTCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         581  GGCCCC               GG[ACGT]^[ACGT]CC
RsaI          470  GTAC                 GT^AC
Sau96I        581  GGCCC                G^G[ACGT]CC
SfaNI         265  GATGC                GCATC(5/9)
TseI          354  GCTGC                G^C[AT]GC
Tsp45I        523  GTGAC                ^GT[GC]AC
Tsp509I        48  AATT                 ^AATT
XmnI           46  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          108  AGCT                 AG^CT
              353  AGCT                
BsaJI         307  CCTTGG               C^C[ACGT][ACGT]GG
              514  CCAAGG              
BstF5I        511  CATCC                GGATG(2/0)
              285  GGATG               
EcoT14I       307  CCTTGG               C^C[AT][AT]GG
              514  CCAAGG              
FokI          511  CATCC                GGATG(9/13)
              285  GGATG               
HaeIII        116  GGCC                 GG^CC
              581  GGCC                
HphI          522  GGTGA                GGTGA(8/7)
              183  TCACC               
MnlI          373  CCTC                 CCTC(7/6)
               33  GAGG                
MseI           26  TTAA                 T^TAA
              488  TTAA                
MslI          240  CACAGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              259  CATGTTGATG          
StyI          307  CCTTGG               C^C[AT][AT]GG
              514  CCAAGG              
Tru9I          26  TTAA                 T^TAA
              488  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BsrI        MaeIII      MboII       NlaIII