Restriction map for
pFN21AE5451
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# NO CUT ENZYMES ( 154 enzymes) #
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Acc65I AccI AccIII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 198 GACGTC GACGT^C
ApaI 495 GGGCCC GGGCC^C
BamHI 651 GGATCC G^GATCC
BanII 495 GGGCCC G[GA]GC[CT]^C
BbsI 434 GAAGAC GAAGAC(2/6)
BcnI 474 CCGGG CC^[GC]GG
BmyI 495 GGGCCC G[AGT]GC[ACT]^C
BpuAI 434 GAAGAC GAAGAC(2/6)
BsmBI 200 CGTCTC CGTCTC(1/5)
BsmFI 6 GGGAC GGGAC(10/14)
Bsp1286I 495 GGGCCC G[AGT]GC[ACT]^C
Cac8I 257 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 81 GCGC GCG^C
DraIII 781 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 66 GACTATCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 53 CCTTGG C^C[AT][AT]GG
FauI 4 GCGGG CCCGC(4/6)
HapII 474 CCGG C^CGG
HgaI 8 GACGC GACGC(5/10)
HhaI 81 GCGC GCG^C
HinP1I 81 GCGC G^CGC
HindIII 955 AAGCTT A^AGCTT
HinfI 1024 GACTC G^A[ACGT]TC
HpaII 474 CCGG C^CGG
MspI 474 CCGG C^CGG
NciI 474 CCGGG CC^[GC]GG
NspI 387 GCATGT [GA]CATG^[CT]
PleI 1024 GACTC GAGTC(4/5)
PpuMI 73 AGGTCCT [GA]G^G[AT]CC[CT]
SbfI 448 CCTGCAGG CCTGCA^GG
Sse8387I 448 CCTGCAGG CCTGCA^GG
StyI 53 CCTTGG C^C[AT][AT]GG
TaqI 816 TCGA T^CGA
TthHB8I 816 TCGA T^CGA
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcsI 177 AAATTC [GA]^AATT[CT]
688 GAATTT
AcyI 8 GACGCC G[GA]^CG[CT]C
198 GACGTC
AlwNI 302 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
845 CAGAAGCTG
ApoI 177 AAATTC [GA]^AATT[CT]
688 GAATTT
AvaII 74 GGTCC G^G[AT]CC
319 GGACC
BbvI 83 GCTGC GCAGC(8/12)
508 GCTGC
BfaI 784 CTAG C^TAG
898 CTAG
BpmI 905 CTGGAG CTGGAG(16/14)
1014 CTGGAG
BsaHI 8 GACGCC G[GA]^CG[CT]C
198 GACGTC
DraII 73 AGGTCCT [GA]G^G[ACGT]CC[CT]
494 GGGGCCC
DsaI 32 CCGCGG C^C[GA][CT]GG
87 CCGCGG
EarI 766 CTCTTC CTCTTC(1/4)
735 GAAGAG
EcoNI 15 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
205 CCTTACCCAGG
EcoO109I 73 AGGTCCT [GA]G^G[ACGT]CC[CT]
494 GGGGCCC
EcoT22I 562 ATGCAT ATGCA^T
830 ATGCAT
Hin1I 8 GACGCC G[GA]^CG[CT]C
198 GACGTC
HphI 694 GGTGA GGTGA(8/7)
874 GGTGA
Ksp632I 766 CTCTTC CTCTTC(1/4)
735 GAAGAG
KspI 32 CCGCGG CCGC^GG
87 CCGCGG
MaeI 784 CTAG C^TAG
898 CTAG
MaeII 146 ACGT A^CGT
199 ACGT
MaeIII 64 GTGAC ^GT[ACGT]AC
968 GTCAC
MseI 137 TTAA T^TAA
371 TTAA
MspA1I 32 CCGCGG C[AC]G^C[GT]G
87 CCGCGG
NsiI 562 ATGCAT ATGCA^T
830 ATGCAT
SacII 32 CCGCGG CCGC^GG
87 CCGCGG
Tru9I 137 TTAA T^TAA
371 TTAA
TseI 83 GCTGC G^C[AT]GC
508 GCTGC
Tsp45I 64 GTGAC ^GT[GC]AC
968 GTCAC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AciI AluI AlwI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI BstUI BstYI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII ItaI MboI MboII MflI
MnlI MvaI MvnI MwoI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI Tsp509I TspRI
XhoII