(Linear) (Six-base) MAPSORT of: KIEE5451.seq Check: 3873 from: 1 to: 1050 KIEE5451.orf With 182 enzymes: SgfI * January 29, 2014 12:01 .. AatII G_ACGT'C Cuts at: 0 202 1050 Size: 202 848 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 753 973 1049 1050 Size: 753 220 76 1 Fragments arranged by size: 753 220 76 1 AlwNI CAG_nnn'CTG Cuts at: 0 307 850 1050 Size: 307 543 200 Fragments arranged by size: 543 307 200 ApaI G_GGCC'C Cuts at: 0 499 1050 Size: 499 551 ApoI r'AATT_y Cuts at: 0 177 688 1050 Size: 177 511 362 Fragments arranged by size: 511 362 177 BamHI G'GATC_C Cuts at: 0 651 1050 Size: 651 399 BanII G_rGCy'C Cuts at: 0 499 1050 Size: 499 551 BbsI GAAGACnn'nnnn_ Cuts at: 0 441 1050 Size: 441 609 BfrBI ATG'CAT Cuts at: 0 564 832 1050 Size: 564 268 218 Fragments arranged by size: 564 268 218 Bme1580I G_kGCm'C Cuts at: 0 499 1050 Size: 499 551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 926 1035 1050 Size: 926 109 15 Fragments arranged by size: 926 109 15 BsaHI Gr'CG_yC Cuts at: 0 9 199 1050 Size: 9 190 851 Fragments arranged by size: 851 190 9 BsmBI CGTCTCn'nnnn_ Cuts at: 0 206 1050 Size: 206 844 Bsp1286I G_dGCh'C Cuts at: 0 499 1050 Size: 499 551 BstYI r'GATC_y Cuts at: 0 333 513 651 1050 Size: 333 180 138 399 Fragments arranged by size: 399 333 180 138 BtgI C'CryG_G Cuts at: 0 32 87 1050 Size: 32 55 963 Fragments arranged by size: 963 55 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 504 1050 Size: 504 546 BtsI GCAGTG_nn' Cuts at: 0 296 552 1050 Size: 296 256 498 Fragments arranged by size: 498 296 256 DraIII CAC_nnn'GTG Cuts at: 0 786 1050 Size: 786 264 DrdI GACnn_nn'nnGTC Cuts at: 0 72 1050 Size: 72 978 EarI CTCTTCn'nnn_ Cuts at: 0 730 772 1050 Size: 730 42 278 Fragments arranged by size: 730 278 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 753 926 973 1035 1049 1050 Size: 753 173 47 62 14 1 Fragments arranged by size: 753 173 62 47 14 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 19 209 1050 Size: 19 190 841 Fragments arranged by size: 841 190 19 EcoO109I rG'GnC_Cy Cuts at: 0 74 495 1050 Size: 74 421 555 Fragments arranged by size: 555 421 74 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 603 1050 Size: 603 447 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 636 668 1050 Size: 636 32 382 Fragments arranged by size: 636 382 32 HindIII A'AGCT_T Cuts at: 0 955 1050 Size: 955 95 MspA1I CmG'CkG Cuts at: 0 34 89 1050 Size: 34 55 961 Fragments arranged by size: 961 55 34 NsiI A_TGCA'T Cuts at: 0 566 834 1050 Size: 566 268 216 Fragments arranged by size: 566 268 216 NspI r_CATG'y Cuts at: 0 391 1050 Size: 391 659 PasI CC'CwG_GG Cuts at: 0 637 1050 Size: 637 413 PfoI T'CCnGG_A Cuts at: 0 654 1050 Size: 654 396 PpuMI rG'GwC_Cy Cuts at: 0 74 1050 Size: 74 976 PsiI TTA'TAA Cuts at: 0 523 1050 Size: 523 527 PspOMI G'GGCC_C Cuts at: 0 495 1050 Size: 495 555 PstI C_TGCA'G Cuts at: 0 303 453 513 1050 Size: 303 150 60 537 Fragments arranged by size: 537 303 150 60 SacII CC_GC'GG Cuts at: 0 35 90 1050 Size: 35 55 960 Fragments arranged by size: 960 55 35 SbfI CC_TGCA'GG Cuts at: 0 453 1050 Size: 453 597 SfcI C'TryA_G Cuts at: 0 299 449 509 1050 Size: 299 150 60 541 Fragments arranged by size: 541 299 150 60 StyI C'CwwG_G Cuts at: 0 53 1050 Size: 53 997 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 453 921 1050 Size: 453 468 129 Fragments arranged by size: 468 453 129 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 603 635 1050 Size: 603 32 415 Fragments arranged by size: 603 415 32 ZraI GAC'GTC Cuts at: 0 200 1050 Size: 200 850 Enzymes that do cut: AatII AcuI AlwNI ApaI ApoI BamHI BanII BbsI BfrBI Bme1580I BpmI BsaHI BsmBI Bsp1286I BstYI BtgI BtgZI BtsI DraIII DrdI EarI Eco57MI EcoNI EcoO109I FalI Hin4I HindIII MspA1I NsiI NspI PasI PfoI PpuMI PsiI PspOMI PstI SacII SbfI SfcI StyI TaqII TstI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI EaeI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PciI PflMI PmeI PmlI PpiI PshAI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI