################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccII AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaHI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MluI MseI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tru9I Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 164 TCCGGA T^CCGGA AciI 407 CCGC CCGC(-3/-1) AclI 337 AACGTT AA^CGTT AcsI 660 GAATTC [GA]^AATT[CT] AfaI 930 GTAC GT^AC AlwNI 626 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 660 GAATTC [GA]^AATT[CT] BbsI 458 GTCTTC GAAGAC(2/6) BpuAI 458 GTCTTC GAAGAC(2/6) BsaWI 164 TCCGGA [AT]^CCGG[AT] BseAI 164 TCCGGA T^CCGGA BsgI 762 GTGCAG GTGCAG(16/14) BspEI 164 TCCGGA T^CCGGA BsrDI 201 CATTGC GCAATG(2/0) BstF5I 148 GGATG GGATG(2/0) BstXI 518 CCATTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 605 GGGTCCT [GA]G^G[ACGT]CC[CT] EarI 883 CTCTTC CTCTTC(1/4) EcoO109I 605 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 660 GAATTC G^AATTC FokI 148 GGATG GGATG(9/13) Ksp632I 883 CTCTTC CTCTTC(1/4) MaeIII 715 GTCAC ^GT[ACGT]AC MfeI 9 CAATTG C^AATTG MroI 164 TCCGGA T^CCGGA MspA1I 889 CAGCTG C[AC]G^C[GT]G MunI 9 CAATTG C^AATTG PaeR7I 1099 CTCGAG C^TCGAG PpuMI 605 GGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 337 AACGTT AA^CGTT PvuII 889 CAGCTG CAG^CTG RsaI 930 GTAC GT^AC SexAI 445 ACCTGGT A^CC[AT]GGT SfiI 1067 GGCCACCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 318 CCCGGG CCC^GGG SspI 796 AATATT AAT^ATT TfiI 468 GAATC G^A[AT]TC Tsp45I 715 GTCAC ^GT[GC]AC XhoI 1099 CTCGAG C^TCGAG XmaCI 318 CCCGGG C^CCGGG XmaI 318 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AvaI 318 CCCGGG C^[CT]CG[GA]G 1099 CTCGAG BanI 325 GGCACC G^G[CT][GA]CC 1051 GGTGCC BcnI 318 CCCGG CC^[GC]GG 319 CCGGG BglI 317 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1068 GCCACCAGGGC BlpI 23 GCTCAGC GC^T[ACGT]AGC 838 GCTGAGC Bpu1102I 23 GCTCAGC GC^T[ACGT]AGC 838 GCTGAGC BsaBI 364 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 804 GATGTGCATC BsaI 189 GAGACC GGTCTC(1/5) 1018 GAGACC BsmAI 189 GAGAC GTCTC(1/5) 1018 GAGAC BsmFI 66 GGGAC GGGAC(10/14) 210 GGGAC BsoBI 318 CCCGGG C^[CT]CG[GA]G 1099 CTCGAG Cac8I 28 GCCTGC GC[ACGT]^[ACGT]GC 729 GCTGGC CelII 23 GCTCAGC GC^T[ACGT]AGC 838 GCTGAGC DsaI 155 CCGTGG C^C[GA][CT]GG 401 CCGTGG HapII 165 CCGG C^CGG 319 CCGG HpaII 165 CCGG C^CGG 319 CCGG MaeII 338 ACGT A^CGT 928 ACGT MamI 364 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 804 GATGTGCATC MspI 165 CCGG C^CGG 319 CCGG NciI 318 CCCGG CC^[GC]GG 319 CCGGG PleI 881 GACTC GAGTC(4/5) 456 GAGTC SacI 91 GAGCTC GAGCT^C 788 GAGCTC SmlI 580 CTCAAG C^T[CT][GA]AG 1099 CTCGAG TaqI 1003 TCGA T^CGA 1100 TCGA TthHB8I 1003 TCGA T^CGA 1100 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AluI AlwI AspHI AvaII BalI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstYI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI HaeIII HinfI HphI ItaI MboI MboII MflI MluNI MnlI MscI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp509I TspRI XcmI XhoII