(Circular) (Six-base) MAPSORT of: pFN21AE6388.seq Check: 9908 from: 1 to: 5836 pFN21AE6388 5836 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 2343 3963 4336 1563 Size: 780 1620 373 3063 Fragments arranged by size: 3063 1620 780 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2300 2450 3899 5283 5551 1632 Size: 668 150 1449 1384 268 1917 Fragments arranged by size: 1917 1449 1384 668 268 150 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 2111 1166 Size: 945 4891 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2837 1184 Size: 1653 4183 AlwNI CAG_nnn'CTG Cuts at: 2636 3579 5151 2636 Size: 943 1572 3321 Fragments arranged by size: 3321 1572 943 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 2665 3145 3371 3523 5632 1493 Size: 1172 480 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 480 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2323 3104 3161 1870 Size: 73 380 781 57 4545 Fragments arranged by size: 4545 781 380 73 57 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2330 3056 3157 4612 618 Size: 325 217 627 543 726 101 1455 1842 Fragments arranged by size: 1842 1455 726 627 543 325 217 101 BanII G_rGCy'C Cuts at: 1391 2100 2238 2797 3103 3155 1391 Size: 709 138 559 306 52 4072 Fragments arranged by size: 4072 709 559 306 138 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2456 928 Size: 971 557 4308 Fragments arranged by size: 4308 971 557 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4027 4061 1903 Size: 34 2090 34 3678 Fragments arranged by size: 3678 2090 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2328 3079 4447 Size: 107 122 71 631 605 656 751 1368 Cuts at: 4447 136 Size: 1525 Fragments arranged by size: 1525 1368 751 656 631 605 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 2029 2844 3253 2029 Size: 815 409 4612 Fragments arranged by size: 4612 815 409 Bme1580I G_kGCm'C Cuts at: 1790 2333 3897 5053 1790 Size: 543 1564 1156 2573 Fragments arranged by size: 2573 1564 1156 543 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2114 2479 2810 2811 4496 1239 Size: 857 18 365 331 1 1685 2579 Fragments arranged by size: 2579 1685 857 365 331 18 1 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2570 3961 4826 5124 2570 Size: 1391 865 298 3282 Fragments arranged by size: 3282 1391 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2188 3017 4499 882 Size: 617 689 829 1482 2219 Fragments arranged by size: 2219 1482 829 689 617 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 2373 2813 5830 2373 Size: 440 3017 2379 Fragments arranged by size: 3017 2379 440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3643 4025 4660 275 Size: 53 83 186 936 2110 382 635 1451 Fragments arranged by size: 2110 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2169 4269 4667 4941 5088 5499 2169 Size: 2100 398 274 147 411 2506 Fragments arranged by size: 2506 2100 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5611 Fragments arranged by size: 5611 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2072 2956 1186 Size: 883 3 884 4066 Fragments arranged by size: 4066 884 883 3 BseYI C'CCAG_C Cuts at: 5039 5535 5039 Size: 496 5340 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2788 1163 Size: 573 1052 4211 Fragments arranged by size: 4211 1052 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 2797 2850 3897 3982 5053 2100 Size: 697 53 1047 85 1071 2883 Fragments arranged by size: 2883 1071 1047 697 85 53 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2238 2333 2797 2850 3103 3155 Size: 399 310 138 95 464 53 253 52 Cuts at: 3155 3897 3982 5053 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 464 399 310 253 138 95 85 53 52 BspEI T'CCGG_A Cuts at: 2169 5499 2169 Size: 3330 2506 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3191 844 Size: 2347 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2205 4331 4505 65 Size: 1183 957 2126 174 1396 Fragments arranged by size: 2126 1396 1183 957 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4480 4667 1700 Size: 110 2670 187 2869 Fragments arranged by size: 2869 2670 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5836 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 2530 2530 Size: 5836 BstYI r'GATC_y Cuts at: 1972 2166 2374 2657 3166 3533 3918 3935 5387 Size: 194 208 283 509 367 385 17 1452 Cuts at: 5387 5473 5485 5831 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 509 385 367 346 283 208 194 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2160 2406 513 Size: 552 1095 246 3943 Fragments arranged by size: 3943 1095 552 246 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 2838 3352 4146 4166 2838 Size: 514 794 20 4508 Fragments arranged by size: 4508 794 514 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2738 2773 529 Size: 35 2174 35 3592 Fragments arranged by size: 3592 2174 35 35 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 2409 2561 Size: 54 1425 50 275 18 432 147 152 Cuts at: 2561 2713 3115 3283 4172 5801 8 Size: 152 402 168 889 1629 43 Fragments arranged by size: 1629 1425 889 432 402 275 168 152 152 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2894 3766 1353 Size: 1541 872 3423 Fragments arranged by size: 3423 1541 872 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4423 4797 4943 387 Size: 1064 138 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2098 2795 2098 Size: 697 5139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2114 2300 2450 2479 2810 2811 Size: 393 464 18 186 150 29 331 1 Cuts at: 2811 3899 4496 5283 5551 1239 Size: 1088 597 787 268 1524 Fragments arranged by size: 1524 1088 787 597 464 393 331 268 186 150 29 18 1 EcoO109I rG'GnC_Cy Cuts at: 2611 2611 Size: 5836 EcoRI G'AATT_C Cuts at: 2665 3145 3523 2665 Size: 480 378 4978 Fragments arranged by size: 4978 480 378 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 4983 1678 Size: 3305 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4477 4509 4551 4583 1373 Size: 32 135 32 2905 32 42 32 2626 Fragments arranged by size: 2905 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 2014 3435 2014 Size: 1421 4415 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2264 2563 2715 3117 10 Size: 54 1425 343 432 299 152 402 2729 Fragments arranged by size: 2729 1425 432 402 343 299 152 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 2123 2210 2387 2528 2819 3794 Size: 648 945 12 87 177 141 291 975 Cuts at: 3794 4153 4312 518 Size: 359 159 2042 Fragments arranged by size: 2042 975 945 648 359 291 177 159 141 87 12 MspA1I CmG'CkG Cuts at: 1606 2896 3252 3929 5077 5322 1606 Size: 1290 356 677 1148 245 2120 Fragments arranged by size: 2120 1290 1148 677 356 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5284 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 2199 2416 1348 Size: 851 217 4768 Fragments arranged by size: 4768 851 217 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5836 PfoI T'CCnGG_A Cuts at: 2456 2456 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2611 2611 Size: 5836 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 3188 830 Size: 2358 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2896 2896 Size: 5836 SacI G_AGCT'C Cuts at: 2100 2797 2100 Size: 697 5139 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SexAI A'CCwGG_T Cuts at: 2450 2450 Size: 5836 SfcI C'TryA_G Cuts at: 826 1045 3184 4319 5000 5191 826 Size: 219 2139 1135 681 191 1471 Fragments arranged by size: 2139 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3079 3079 Size: 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 2325 3163 1872 Size: 453 838 4545 Fragments arranged by size: 4545 838 453 SmlI C'TyrA_G Cuts at: 820 1017 1943 2585 3104 3940 4841 5103 820 Size: 197 926 642 519 836 901 262 1553 Fragments arranged by size: 1553 926 901 836 642 519 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 2803 3760 5 Size: 47 2751 957 2081 Fragments arranged by size: 2751 2081 957 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 3275 513 Size: 552 2210 3074 Fragments arranged by size: 3074 2210 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3863 3880 4033 4218 4663 325 Size: 1255 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4082 5794 96 Size: 274 80 33 51 1443 2105 1712 138 Fragments arranged by size: 2105 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2064 2096 2145 2177 5796 5828 2064 Size: 32 49 32 3619 32 2072 Fragments arranged by size: 3619 2072 49 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2125 2900 3034 2125 Size: 775 134 4927 Fragments arranged by size: 4927 775 134 XhoI C'TCGA_G Cuts at: 1943 3104 1943 Size: 1161 4675 XmaI C'CCGG_G Cuts at: 1870 2323 3161 1870 Size: 453 838 4545 Fragments arranged by size: 4545 838 453 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI