Restriction map for
pFN21AE4216
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
AvrII BalI BbeI BbrPI BcgI
BclI BcnI BglI BglII BlnI
BsaAI BsaHI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HaeII HapII HgaI Hin1I
HindIII HpaII KasI KspI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuII RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1405 GGTACC G^GTACC
AcsI 1043 AAATTC [GA]^AATT[CT]
AflII 631 CTTAAG C^TTAAG
Alw44I 1925 GTGCAC G^TGCAC
AlwNI 403 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1530 CCTGAGG CC^T[ACGT]AGG
ApaI 1783 GGGCCC GGGCC^C
ApaLI 1925 GTGCAC G^TGCAC
ApoI 1043 AAATTC [GA]^AATT[CT]
Asp718I 1405 GGTACC G^GTACC
AviII 1445 TGCGCA TGC^GCA
BamHI 1168 GGATCC G^GATCC
BanI 1405 GGTACC G^G[CT][GA]CC
BbsI 340 GAAGAC GAAGAC(2/6)
BbvCI 75 GCTGAGG CCTCAGC(-5/-2)
BciVI 414 GGATAC GTATCC(6/5)
BfrI 631 CTTAAG C^TTAAG
BlpI 711 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 711 GCTCAGC GC^T[ACGT]AGC
BpuAI 340 GAAGAC GAAGAC(2/6)
BsaBI 274 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 514 GGTCTC GGTCTC(1/5)
BsiEI 273 CGATCG CG[GA][CT]^CG
BsmI 54 GAATGC GAATGC(1/-1)
BspHI 297 TCATGA T^CATGA
BsrDI 224 GCAATG GCAATG(2/0)
BstBI 1493 TTCGAA TT^CGAA
BstXI 170 CCAAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1530 CCTGAGG CC^T[ACGT]AGG
CelII 711 GCTCAGC GC^T[ACGT]AGC
DraIII 613 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1530 CCTGAGG CC^T[ACGT]AGG
FauI 1788 CCCGC CCCGC(4/6)
FspI 1445 TGCGCA TGC^GCA
HincII 838 GTTAAC GT[CT]^[GA]AC
HindII 838 GTTAAC GT[CT]^[GA]AC
HpaI 838 GTTAAC GTT^AAC
KpnI 1405 GGTACC GGTAC^C
MaeII 2182 ACGT A^CGT
MamI 274 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1061 CCATGG C^CATGG
NdeI 369 CATATG CA^TATG
NspI 2147 ACATGC [GA]CATG^[CT]
NspV 1493 TTCGAA TT^CGAA
PvuI 273 CGATCG CGAT^CG
RcaI 297 TCATGA T^CATGA
SexAI 1660 ACCTGGT A^CC[AT]GGT
SfuI 1493 TTCGAA TT^CGAA
XbaI 1237 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 256 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2073 GAAAAAGTTC
AvaI 831 CTCGGG C^[CT]CG[GA]G
1474 CTCGGG
BpmI 409 CTGGAG CTGGAG(16/14)
1559 CTGGAG
BsgI 1930 CTGCAC GTGCAG(16/14)
853 GTGCAG
BsmAI 1891 GAGAC GTCTC(1/5)
515 GTCTC
BsoBI 831 CTCGGG C^[CT]CG[GA]G
1474 CTCGGG
Bsp1407I 609 TGTACA T^GTACA
1428 TGTACA
BsrGI 609 TGTACA T^GTACA
1428 TGTACA
BstYI 906 AGATCC [GA]^GATC[CT]
1168 GGATCC
CfoI 891 GCGC GCG^C
1446 GCGC
DsaI 20 CCACGG C^C[GA][CT]GG
1061 CCATGG
EcoNI 379 CCTTTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1950 CCTGAAACAGG
HhaI 891 GCGC GCG^C
1446 GCGC
HinP1I 891 GCGC G^CGC
1446 GCGC
MflI 906 AGATCC [GA]^GATC[CT]
1168 GGATCC
MslI 930 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1837 CACAGGAGTG
SacI 553 GAGCTC GAGCT^C
1383 GAGCTC
SapI 1385 GCTCTTC GCTCTTC(1/4)
1715 GCTCTTC
SfcI 1369 CTACAG C^T[GA][CT]AG
2152 CTACAG
SspBI 609 TGTACA T^GTACA
1428 TGTACA
TfiI 806 GATTC G^A[AT]TC
2238 GATTC
XhoII 906 AGATCC [GA]^GATC[CT]
1168 GGATCC
XmnI 256 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2073 GAAAAAGTTC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BfaI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I