Restriction map for
pFN21AE4216
(Circular) (Six-base) MAPSORT of: pFN21AE4216.seq Check: 6505 from: 1 to: 6949
pFN21AE4216 6949 bp
With 182 enzymes: SgfI *
March 28, 2012 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2749 3743 2726
Size: 23 994 5932
Fragments arranged by size:
5932 994 23
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4759 278
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4292 1937
Size: 2355 4594
Acc65I G'GTAC_C
Cuts at: 3410 4270 3410
Size: 860 6089
AclI AA'CG_TT
Cuts at: 1563 5076 5449 1563
Size: 3513 373 3063
Fragments arranged by size:
3513 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3301 3419 5012 6396 6664 1632
Size: 1669 118 1593 1384 268 1917
Fragments arranged by size:
1917 1669 1593 1384 268 118
AflII C'TTAA_G
Cuts at: 820 1017 2636 820
Size: 197 1619 5133
Fragments arranged by size:
5133 1619 197
AflIII A'CryG_T
Cuts at: 1203 5848 6825 1203
Size: 4645 977 1327
Fragments arranged by size:
4645 1327 977
AgeI A'CCGG_T
Cuts at: 5780 5780
Size: 6949
AhdI GACnn_n'nnGTC
Cuts at: 5678 5678
Size: 6949
AleI CACnn'nnGTG
Cuts at: 1166 2939 3846 1166
Size: 1773 907 4269
Fragments arranged by size:
4269 1773 907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6949
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2006 2038 3158 3190 3695 3727 2006
Size: 32 1120 32 505 32 5228
Fragments arranged by size:
5228 1120 505 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2413 4692 6264 2413
Size: 2279 1572 3098
Fragments arranged by size:
3098 2279 1572
ApaI G_GGCC'C
Cuts at: 3792 3792
Size: 6949
ApaLI G'TGCA_C
Cuts at: 3930 5006 6162 3930
Size: 1076 1156 4717
Fragments arranged by size:
4717 1156 1076
ApoI r'AATT_y
Cuts at: 1493 3048 4258 4484 4636 6745 1493
Size: 1555 1210 226 152 2109 1697
Fragments arranged by size:
2109 1697 1555 1210 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5503 160
Size: 872 4471 1606
Fragments arranged by size:
4471 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
AvaI C'yCGr_G
Cuts at: 1870 1943 2836 3479 4274 1870
Size: 73 893 643 795 4545
Fragments arranged by size:
4545 893 795 643 73
BamHI G'GATC_C
Cuts at: 3173 4279 4646 3173
Size: 1106 367 5476
Fragments arranged by size:
5476 1106 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3410 4270 5725 618
Size: 325 217 627 1623 860 1455 1842
Fragments arranged by size:
1842 1623 1455 860 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2562 3392 3792 4268 1391
Size: 1171 830 400 476 4072
Fragments arranged by size:
4072 1171 830 476 400
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2352 928
Size: 971 453 5525
Fragments arranged by size:
5525 971 453
BbvCI CC'TCA_GC
Cuts at: 2081 2081
Size: 6949
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5140 5174 1903
Size: 34 3203 34 3678
Fragments arranged by size:
3678 3203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2413 4841 6051 2413
Size: 2428 1210 3311
Fragments arranged by size:
3311 2428 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6949
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5560 136
Size: 107 122 71 631 605 3888 1525
Fragments arranged by size:
3888 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6944 6944
Size: 6949
BlpI GC'TnA_GC
Cuts at: 2717 4366 2717
Size: 1649 5300
Bme1580I G_kGCm'C
Cuts at: 1790 2936 3425 3792 3934 5010 6166 1790
Size: 1146 489 367 142 1076 1156 2573
Fragments arranged by size:
2573 1156 1146 1076 489 367 142
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6949
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2359 4680 5638
Size: 416 53 83 399 24 942 2321 958
Cuts at: 5638 442
Size: 1753
Fragments arranged by size:
2321 1753 958 942 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2435 3585 5609 1239
Size: 1196 1150 2024 2579
Fragments arranged by size:
2579 2024 1196 1150
Bpu10I CC'TnA_GC
Cuts at: 2081 2081
Size: 6949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 2780 3362 5074 5939 6237 2117
Size: 663 582 1712 865 298 2829
Fragments arranged by size:
2829 1712 865 663 582 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2525 5612 882
Size: 617 1026 3087 2219
Fragments arranged by size:
3087 2219 1026 617
BsaAI yAC'GTr
Cuts at: 493 1204 4740 493
Size: 711 3536 2702
Fragments arranged by size:
3536 2702 711
BsaBI GATnn'nnATC
Cuts at: 2283 6943 2283
Size: 4660 2289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4756 5138 5773 275
Size: 53 83 186 936 3223 382 635 1451
Fragments arranged by size:
3223 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5382 5780 6054 6201 6612 5382
Size: 398 274 147 411 5719
Fragments arranged by size:
5719 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2006 2036 2085 2115 3695
Size: 30 165 30 603 30 49 30 1580
Cuts at: 3695 3725 1178
Size: 30 4402
Fragments arranged by size:
4402 1580 603 165 49 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2306 2406 2783 1186
Size: 838 282 100 377 5352
Fragments arranged by size:
5352 838 377 282 100
BseYI C'CCAG_C
Cuts at: 3274 6152 6648 3274
Size: 2878 496 3575
Fragments arranged by size:
3575 2878 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2879 3920 1163
Size: 573 1143 1041 4192
Fragments arranged by size:
4192 1143 1041 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2281 4399 5160 6188 1540
Size: 275 186 280 2118 761 1028 2301
Fragments arranged by size:
2301 2118 1028 761 280 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 2562 3128 3392 3934 5010 5095 6166 2133
Size: 429 566 264 542 1076 85 1071 2916
Fragments arranged by size:
2916 1076 1071 566 542 429 264 85
BsmI GAATG_Cn'
Cuts at: 2065 4460 4553 2065
Size: 2395 93 4461
Fragments arranged by size:
4461 2395 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2562 2936 3128 3392 3425 3792
Size: 399 343 429 374 192 264 33 367
Cuts at: 3792 3934 4268 5010 5095 6166 1391
Size: 142 334 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 374 367 343
334 264 192 142 85 33
BspEI T'CCGG_A
Cuts at: 6612 6612
Size: 6949
BspHI T'CATG_A
Cuts at: 2302 4838 6568 2302
Size: 2536 1730 2683
Fragments arranged by size:
2683 2536 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2726 2749 3743 4304 844
Size: 1882 23 994 561 3489
Fragments arranged by size:
3489 1882 994 561 23
BsrBI CCG'CTC
Cuts at: 1394 1823 4836 1394
Size: 429 3013 3507
Fragments arranged by size:
3507 3013 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2236 5444 5618 65
Size: 1183 988 3208 174 1396
Fragments arranged by size:
3208 1396 1183 988 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5593 5780 1700
Size: 110 3783 187 2869
Fragments arranged by size:
3783 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2614 3433 96
Size: 2518 819 3612
Fragments arranged by size:
3612 2518 819
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6949
BssSI C'ACGA_G
Cuts at: 5009 6021 5009
Size: 1012 5937
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6949
BstBI TT'CG_AA
Cuts at: 3499 5753 3499
Size: 2254 4695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6949
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 2182
Size: 6949
BstYI r'GATC_y
Cuts at: 1972 2911 3173 4279 4646 5031 5048 6500 6586
Size: 939 262 1106 367 385 17 1452 86
Cuts at: 6586 6598 6944 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1106 939 385 367 346 262
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3536 3536
Size: 6949
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 3066 513
Size: 552 939 21 1041 4396
Fragments arranged by size:
4396 1041 939 552 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6210
Fragments arranged by size:
6210 683 56
BtsI GCAGTG_nn'
Cuts at: 2483 3128 3889 3932 4465 5259 5279 2483
Size: 645 761 43 533 794 20 4153
Fragments arranged by size:
4153 794 761 645 533 43 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6914
DraI TTT'AAA
Cuts at: 4253 4253
Size: 6949
DraIII CAC_nnn'GTG
Cuts at: 2623 2623
Size: 6949
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5956 809
Size: 507 4640 1802
Fragments arranged by size:
4640 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4396 5285 6914
Size: 54 1425 50 275 18 2566 889 1629
Cuts at: 6914 8
Size: 43
Fragments arranged by size:
2566 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4396 1537
Size: 275 2584 4090
Fragments arranged by size:
4090 2584 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2229 2432 2508 2721 2759 3397 3727 4879
Size: 876 203 76 213 38 638 330 1152
Cuts at: 4879 1353
Size: 3423
Fragments arranged by size:
3423 1152 876 638 330 213 203 76
38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2354 5536 5910 6056 387
Size: 1064 138 765 3182 374 146 1280
Fragments arranged by size:
3182 1280 1064 765 374 146 138
EcoICRI GAG'CTC
Cuts at: 2560 3390 2560
Size: 830 6119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2435 3301 3419 3585 5012 5609 6396
Size: 393 803 866 118 166 1427 597 787
Cuts at: 6396 6664 1239
Size: 268 1524
Fragments arranged by size:
1524 1427 866 803 787 597 393 268
166 118
EcoNI CCTnn'n_nnAGG
Cuts at: 2388 3959 2388
Size: 1571 5378
EcoO109I rG'GnC_Cy
Cuts at: 2381 3312 3772 3789 2381
Size: 931 460 17 5541
Fragments arranged by size:
5541 931 460 17
EcoRI G'AATT_C
Cuts at: 4258 4636 4258
Size: 378 6571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3408 3408
Size: 6949
FspI TGC'GCA
Cuts at: 3452 5455 3452
Size: 2003 4946
FspAI rTGC'GCAy
Cuts at: 3452 3452
Size: 6949
HaeII r_GCGC'y
Cuts at: 1678 6096 1678
Size: 4418 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2085 2117 2284 2316 2576
Size: 32 135 32 513 32 167 32 260
Cuts at: 2576 2608 2684 2716 3977 4009 5590 5622 5664
Size: 32 76 32 1261 32 1581 32 42
Cuts at: 5664 5696 1373
Size: 32 2626
Fragments arranged by size:
2626 1581 1261 513 260 167 135 76
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2845 4293 4539 669
Size: 988 281 907 1448 246 3079
Fragments arranged by size:
3079 1448 988 907 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6949
HpaI GTT'AAC
Cuts at: 2845 4539 2845
Size: 1694 5255
KpnI G_GTAC'C
Cuts at: 3414 4274 3414
Size: 860 6089
MfeI C'AATT_G
Cuts at: 4548 4548
Size: 6949
MluI A'CGCG_T
Cuts at: 6825 6825
Size: 6949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2518 3732 6063 6247 1305
Size: 1213 1214 2331 184 2007
Fragments arranged by size:
2331 2007 1214 1213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5127
Fragments arranged by size:
5127 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2939 3846 4907 5266 5425 518
Size: 648 1773 907 1061 359 159 2042
Fragments arranged by size:
2042 1773 1061 907 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4365 5042 6190 6435 1606
Size: 2759 677 1148 245 2120
Fragments arranged by size:
2759 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3066 513
Size: 552 939 1062 4396
Fragments arranged by size:
4396 1062 939 552
NdeI CA'TA_TG
Cuts at: 387 2375 387
Size: 1988 4961
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6949
NotI GC'GGCC_GC
Cuts at: 4396 4396
Size: 6949
NspI r_CATG'y
Cuts at: 1132 1229 4156 4307 5852 1132
Size: 97 2927 151 1545 2229
Fragments arranged by size:
2927 2229 1545 151 97
PasI CC'CwG_GG
Cuts at: 1348 2243 3816 1348
Size: 895 1573 4481
Fragments arranged by size:
4481 1573 895
PciI A'CATG_T
Cuts at: 5848 5848
Size: 6949
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6949
PfoI T'CCnGG_A
Cuts at: 2269 3995 2269
Size: 1726 5223
PmeI GTTT'AAAC
Cuts at: 4253 4253
Size: 6949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2006 2038 3695 3727 5018 5050 6531 6563 2006
Size: 32 1657 32 1291 32 1481 32 2392
Fragments arranged by size:
2392 1657 1481 1291 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2381 3312 3772 2381
Size: 931 460 5558
Fragments arranged by size:
5558 931 460
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6949
PsiI TTA'TAA
Cuts at: 4519 4519
Size: 6949
PspOMI G'GGCC_C
Cuts at: 3788 3788
Size: 6949
PstI C_TGCA'G
Cuts at: 830 4301 830
Size: 3471 3478
PvuI CG_AT'CG
Cuts at: 2001 2281 2001
Size: 280 6669
SacI G_AGCT'C
Cuts at: 2562 3392 2562
Size: 830 6119
SalI G'TCGA_C
Cuts at: 1936 4291 1936
Size: 2355 4594
SapI GCTCTTCn'nnn_
Cuts at: 3397 3727 3397
Size: 330 6619
SbfI CC_TGCA'GG
Cuts at: 4301 4301
Size: 6949
ScaI AGT'ACT
Cuts at: 5197 6909 5197
Size: 1712 5237
SexAI A'CCwGG_T
Cuts at: 3665 3665
Size: 6949
SfcI C'TryA_G
Cuts at: 826 1045 3374 4157 4297 5432 6113 6304 826
Size: 219 2329 783 140 1135 681 191 1471
Fragments arranged by size:
2329 1471 1135 783 681 219 191 140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6949
SmaI CCC'GGG
Cuts at: 1872 4276 1872
Size: 2404 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2636 2795 3341 5053 5954
Size: 197 926 189 504 159 546 1712 901
Cuts at: 5954 6216 820
Size: 262 1553
Fragments arranged by size:
1712 1553 926 901 546 504 262 197
189 159
SnaBI TAC'GTA
Cuts at: 493 4740 493
Size: 4247 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6949
SphI G_CATG'C
Cuts at: 1132 4307 1132
Size: 3175 3774
SspI AAT'ATT
Cuts at: 5 52 4873 5
Size: 47 4821 2081
Fragments arranged by size:
4821 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6949
StyI C'CwwG_G
Cuts at: 513 1065 2004 2047 2181 3066 3102 4388 513
Size: 552 939 43 134 885 36 1286 3074
Fragments arranged by size:
3074 1286 939 885 552 134 43 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2064 2341 2345 4976 4993 5146 5331
Size: 1255 484 277 4 2631 17 153 185
Cuts at: 5331 5776 325
Size: 445 1498
Fragments arranged by size:
2631 1498 1255 484 445 277 185 153
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2614 2824 3433
Size: 274 80 33 51 1443 637 210 609
Cuts at: 3433 5195 6907 96
Size: 1762 1712 138
Fragments arranged by size:
1762 1712 1443 637 609 274 210 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6909 6941 6909
Size: 32 6917
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6949
XbaI T'CTAG_A
Cuts at: 3242 4285 3242
Size: 1043 5906
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6949
XmaI C'CCGG_G
Cuts at: 1870 4274 1870
Size: 2404 4545
XmnI GAAnn'nnTTC
Cuts at: 2265 4082 5078 2265
Size: 1817 996 4136
Fragments arranged by size:
4136 1817 996
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4757 276
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoRV FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacII SanDI SfiI SfoI SrfI SwaI XcmI