(Linear) (Six-base) MAPSORT of: KIEE4216.seq Check: 4271 from: 1 to: 2244 KIEE4216.orf With 182 enzymes: SgfI * March 22, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 721 744 1738 2244 Size: 721 23 994 506 Fragments arranged by size: 994 721 506 23 Acc65I G'GTAC_C Cuts at: 0 1405 2244 Size: 1405 839 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1296 1414 2244 Size: 1296 118 830 Fragments arranged by size: 1296 830 118 AflII C'TTAA_G Cuts at: 0 631 2244 Size: 631 1613 AleI CACnn'nnGTG Cuts at: 0 934 1841 2244 Size: 934 907 403 Fragments arranged by size: 934 907 403 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1 33 1153 1185 1690 1722 2244 Size: 1 32 1120 32 505 32 522 Fragments arranged by size: 1120 522 505 32 32 32 1 AlwNI CAG_nnn'CTG Cuts at: 0 408 2244 Size: 408 1836 ApaI G_GGCC'C Cuts at: 0 1787 2244 Size: 1787 457 ApaLI G'TGCA_C Cuts at: 0 1925 2244 Size: 1925 319 ApoI r'AATT_y Cuts at: 0 1043 2244 Size: 1043 1201 AvaI C'yCGr_G Cuts at: 0 831 1474 2244 Size: 831 643 770 Fragments arranged by size: 831 770 643 BamHI G'GATC_C Cuts at: 0 1168 2244 Size: 1168 1076 BanI G'GyrC_C Cuts at: 0 1405 2244 Size: 1405 839 BanII G_rGCy'C Cuts at: 0 557 1387 1787 2244 Size: 557 830 400 457 Fragments arranged by size: 830 557 457 400 BbsI GAAGACnn'nnnn_ Cuts at: 0 347 2244 Size: 347 1897 BbvCI CC'TCA_GC Cuts at: 0 76 2244 Size: 76 2168 BciVI GTATCCnnnnn_n' Cuts at: 0 408 2244 Size: 408 1836 BlpI GC'TnA_GC Cuts at: 0 712 2244 Size: 712 1532 Bme1580I G_kGCm'C Cuts at: 0 931 1420 1787 1929 2244 Size: 931 489 367 142 315 Fragments arranged by size: 931 489 367 315 142 BmrI ACTGGGnnnn_n' Cuts at: 0 354 2244 Size: 354 1890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 1580 2244 Size: 430 1150 664 Fragments arranged by size: 1150 664 430 Bpu10I CC'TnA_GC Cuts at: 0 76 2244 Size: 76 2168 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 775 1357 2244 Size: 112 663 582 887 Fragments arranged by size: 887 663 582 112 BsaI GGTCTCn'nnnn_ Cuts at: 0 520 2244 Size: 520 1724 BsaBI GATnn'nnATC Cuts at: 0 278 2244 Size: 278 1966 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1 31 80 110 1690 1720 2244 Size: 1 30 49 30 1580 30 524 Fragments arranged by size: 1580 524 49 30 30 30 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 301 401 778 2244 Size: 19 282 100 377 1466 Fragments arranged by size: 1466 377 282 100 19 BseYI C'CCAG_C Cuts at: 0 1269 2244 Size: 1269 975 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 874 1915 2244 Size: 874 1041 329 Fragments arranged by size: 1041 874 329 BsiEI CG_ry'CG Cuts at: 0 276 2244 Size: 276 1968 BsiHKAI G_wGCw'C Cuts at: 0 128 557 1123 1387 1929 2244 Size: 128 429 566 264 542 315 Fragments arranged by size: 566 542 429 315 264 128 BsmI GAATG_Cn' Cuts at: 0 60 2244 Size: 60 2184 Bsp1286I G_dGCh'C Cuts at: 0 128 557 931 1123 1387 1420 1787 1929 Size: 128 429 374 192 264 33 367 142 Cuts at: 1929 2244 Size: 315 Fragments arranged by size: 429 374 367 315 264 192 142 128 33 BspHI T'CATG_A Cuts at: 0 297 2244 Size: 297 1947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 721 744 1738 2244 Size: 721 23 994 506 Fragments arranged by size: 994 721 506 23 BsrDI GCAATG_nn' Cuts at: 0 231 2244 Size: 231 2013 BsrGI T'GTAC_A Cuts at: 0 609 1428 2244 Size: 609 819 816 Fragments arranged by size: 819 816 609 BstBI TT'CG_AA Cuts at: 0 1494 2244 Size: 1494 750 BstXI CCAn_nnnn'nTGG Cuts at: 0 177 2244 Size: 177 2067 BstYI r'GATC_y Cuts at: 0 906 1168 2244 Size: 906 262 1076 Fragments arranged by size: 1076 906 262 Bsu36I CC'TnA_GG Cuts at: 0 1531 2244 Size: 1531 713 BtgI C'CryG_G Cuts at: 0 20 1061 2244 Size: 20 1041 1183 Fragments arranged by size: 1183 1041 20 BtsI GCAGTG_nn' Cuts at: 0 478 1123 1884 1927 2244 Size: 478 645 761 43 317 Fragments arranged by size: 761 645 478 317 43 DraIII CAC_nnn'GTG Cuts at: 0 618 2244 Size: 618 1626 EarI CTCTTCn'nnn_ Cuts at: 0 224 427 503 716 754 1392 1722 2244 Size: 224 203 76 213 38 638 330 522 Fragments arranged by size: 638 522 330 224 213 203 76 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 349 2244 Size: 349 1895 EcoICRI GAG'CTC Cuts at: 0 555 1385 2244 Size: 555 830 859 Fragments arranged by size: 859 830 555 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 1296 1414 1580 2244 Size: 430 866 118 166 664 Fragments arranged by size: 866 664 430 166 118 EcoNI CCTnn'n_nnAGG Cuts at: 0 383 1954 2244 Size: 383 1571 290 Fragments arranged by size: 1571 383 290 EcoO109I rG'GnC_Cy Cuts at: 0 376 1307 1767 1784 2244 Size: 376 931 460 17 460 Fragments arranged by size: 931 460 460 376 17 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1403 2244 Size: 1403 841 FspI TGC'GCA Cuts at: 0 1447 2244 Size: 1447 797 FspAI rTGC'GCAy Cuts at: 0 1447 2244 Size: 1447 797 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 80 112 279 311 571 603 679 711 Size: 80 32 167 32 260 32 76 32 Cuts at: 711 1972 2004 2244 Size: 1261 32 240 Fragments arranged by size: 1261 260 240 167 80 76 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 840 2244 Size: 840 1404 HpaI GTT'AAC Cuts at: 0 840 2244 Size: 840 1404 KpnI G_GTAC'C Cuts at: 0 1409 2244 Size: 1409 835 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 513 1727 2244 Size: 513 1214 517 Fragments arranged by size: 1214 517 513 MslI CAynn'nnrTG Cuts at: 0 934 1841 2244 Size: 934 907 403 Fragments arranged by size: 934 907 403 NcoI C'CATG_G Cuts at: 0 1061 2244 Size: 1061 1183 NdeI CA'TA_TG Cuts at: 0 370 2244 Size: 370 1874 NspI r_CATG'y Cuts at: 0 2151 2244 Size: 2151 93 PasI CC'CwG_GG Cuts at: 0 238 1811 2244 Size: 238 1573 433 Fragments arranged by size: 1573 433 238 PfoI T'CCnGG_A Cuts at: 0 264 1990 2244 Size: 264 1726 254 Fragments arranged by size: 1726 264 254 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1 33 1690 1722 2244 Size: 1 32 1657 32 522 Fragments arranged by size: 1657 522 32 32 1 PpuMI rG'GwC_Cy Cuts at: 0 376 1307 1767 2244 Size: 376 931 460 477 Fragments arranged by size: 931 477 460 376 PspOMI G'GGCC_C Cuts at: 0 1783 2244 Size: 1783 461 PvuI CG_AT'CG Cuts at: 0 276 2244 Size: 276 1968 SacI G_AGCT'C Cuts at: 0 557 1387 2244 Size: 557 830 857 Fragments arranged by size: 857 830 557 SapI GCTCTTCn'nnn_ Cuts at: 0 1392 1722 2244 Size: 1392 330 522 Fragments arranged by size: 1392 522 330 SexAI A'CCwGG_T Cuts at: 0 1660 2244 Size: 1660 584 SfcI C'TryA_G Cuts at: 0 1369 2152 2244 Size: 1369 783 92 Fragments arranged by size: 1369 783 92 SmlI C'TyrA_G Cuts at: 0 127 631 790 1336 2244 Size: 127 504 159 546 908 Fragments arranged by size: 908 546 504 159 127 StyI C'CwwG_G Cuts at: 0 42 176 1061 1097 2244 Size: 42 134 885 36 1147 Fragments arranged by size: 1147 885 134 42 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 59 336 340 2244 Size: 59 277 4 1904 Fragments arranged by size: 1904 277 59 4 TatI w'GTAC_w Cuts at: 0 609 819 1428 2244 Size: 609 210 609 816 Fragments arranged by size: 816 609 609 210 XbaI T'CTAG_A Cuts at: 0 1237 2244 Size: 1237 1007 XmnI GAAnn'nnTTC Cuts at: 0 260 2077 2244 Size: 260 1817 167 Fragments arranged by size: 1817 260 167 Enzymes that do cut: AarI Acc65I AcuI AflII AleI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstBI BstXI BstYI Bsu36I BtgI BtsI DraIII EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI Hin4I HincII HpaI KpnI MmeI MslI NcoI NdeI NspI PasI PfoI PpiI PpuMI PspOMI PvuI SacI SapI SexAI SfcI SmlI StyI TaqII TatI XbaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflIII AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglI BglII BmgBI BmtI BplI BsaAI BsaHI BsaWI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EaeI EagI EcoRI EcoRV FseI HaeII HindIII KasI MfeI MluI MscI MspA1I NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuII RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XcmI XhoI XmaI ZraI