Restriction map for
pFN21AE2315
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiWI BsmBI
BsmI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
MluI MluNI MroI MscI NarI
NcoI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PstI PvuI
PvuII RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1384 GTAGAC GT^[AC][GT]AC
AclI 2788 AACGTT AA^CGTT
AcyI 3164 GACGCC G[GA]^CG[CT]C
AflIII 317 ACATGT A^C[GA][CT]GT
BamHI 1686 GGATCC G^GATCC
BciVI 2156 GTATCC GTATCC(6/5)
BsaBI 1687 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3164 GACGCC G[GA]^CG[CT]C
BsiEI 92 CGGCCG CG[GA][CT]^CG
BsmFI 2499 GGGAC GGGAC(10/14)
Bsp1407I 2549 TGTACA T^GTACA
BspHI 2327 TCATGA T^CATGA
BspLU11I 317 ACATGT A^CATGT
BsrDI 1871 GCAATG GCAATG(2/0)
BsrGI 2549 TGTACA T^GTACA
BssSI 1099 CTCGTG CACGAG(-5/-1)
BstAPI 1122 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1686 GGATCC [GA]^GATC[CT]
EaeI 92 CGGCCG [CT]^GGCC[GA]
EagI 92 CGGCCG C^GGCCG
EclXI 92 CGGCCG C^GGCCG
Eco52I 92 CGGCCG C^GGCCG
EcoNI 882 CCTATCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 79 GGCGCT [GA]GCGC^[CT]
HgaI 3164 GACGC GACGC(5/10)
Hin1I 3164 GACGCC G[GA]^CG[CT]C
MamI 1687 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1686 GGATCC [GA]^GATC[CT]
NaeI 26 GCCGGC GCC^GGC
NdeI 1166 CATATG CA^TATG
NgoMIV 26 GCCGGC G^CCGGC
PshAI 934 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2788 AACGTT AA^CGTT
RcaI 2327 TCATGA T^CATGA
SgrAI 25 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 763 ACTAGT A^CTAGT
SspBI 2549 TGTACA T^GTACA
StuI 1970 AGGCCT AGG^CCT
XbaI 853 TCTAGA T^CTAGA
XhoII 1686 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 90 CGCG CG^CG
165 CGCG
AlwI 1687 GATCC GGATC(4/5)
1686 GGATC
AseI 895 ATTAAT AT^TAAT
3027 ATTAAT
AsnI 895 ATTAAT AT^TAAT
3027 ATTAAT
AvaI 100 CTCGAG C^[CT]CG[GA]G
2879 CTCGAG
BbsI 1510 GAAGAC GAAGAC(2/6)
1646 GTCTTC
BbvI 1119 GCTGC GCAGC(8/12)
2710 GCTGC
BpmI 560 CTGGAG CTGGAG(16/14)
3050 CTGGAG
BpuAI 1510 GAAGAC GAAGAC(2/6)
1646 GTCTTC
BsgI 2004 CTGCAC GTGCAG(16/14)
3086 GTGCAG
BsoBI 100 CTCGAG C^[CT]CG[GA]G
2879 CTCGAG
BsrFI 26 GCCGGC [GA]^CCGG[CT]
177 GCCGGT
BstUI 90 CGCG CG^CG
165 CGCG
Cac8I 26 GCCGGC GC[ACGT]^[ACGT]GC
166 GCGGGC
CfoI 80 GCGC GCG^C
1899 GCGC
Cfr10I 26 GCCGGC [GA]^CCGG[CT]
177 GCCGGT
DraI 1893 TTTAAA TTT^AAA
2085 TTTAAA
EcoRI 1038 GAATTC G^AATTC
2245 GAATTC
HhaI 80 GCGC GCG^C
1899 GCGC
HinP1I 80 GCGC G^CGC
1899 GCGC
HindIII 60 AAGCTT A^AGCTT
2733 AAGCTT
KspI 89 CCGCGG CCGC^GG
164 CCGCGG
MfeI 1175 CAATTG C^AATTG
3146 CAATTG
MspA1I 89 CCGCGG C[AC]G^C[GT]G
164 CCGCGG
MunI 1175 CAATTG C^AATTG
3146 CAATTG
MvnI 90 CGCG CG^CG
165 CGCG
PaeR7I 100 CTCGAG C^TCGAG
2879 CTCGAG
PflMI 1078 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2843 CCAACATTTGG
PshBI 895 ATTAAT AT^TAAT
3027 ATTAAT
SacI 97 GAGCTC GAGCT^C
2060 GAGCTC
SacII 89 CCGCGG CCGC^GG
164 CCGCGG
SfcI 419 CTACAG C^T[GA][CT]AG
2522 CTATAG
TseI 1119 GCTGC G^C[AT]GC
2710 GCTGC
Van91I 1078 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2843 CCAACATTTGG
XhoI 100 CTCGAG C^TCGAG
2879 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AspHI AvaII BanII BclI
BcnI BfaI BmyI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI SspI
StyI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I XmnI