(Circular) (Six-base) MAPSORT of: pFN21AE2315.seq Check: 7123 from: 1 to: 8008 pFN21AE2315 8008 bp With 182 enzymes: SgfI * January 11, 2011 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4019 4019 Size: 8008 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5818 278 Size: 53 83 186 936 4282 2468 Fragments arranged by size: 4282 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3390 5351 1937 Size: 1453 1961 4594 Fragments arranged by size: 4594 1961 1453 Acc65I G'GTAC_C Cuts at: 5329 5329 Size: 8008 AclI AA'CG_TT Cuts at: 1563 4794 6135 6508 1563 Size: 3231 1341 373 3063 Fragments arranged by size: 3231 3063 1341 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4485 4657 5154 6071 7455 7723 1632 Size: 2853 172 497 917 1384 268 1917 Fragments arranged by size: 2853 1917 1384 917 497 268 172 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7811 AflIII A'CryG_T Cuts at: 1203 2322 6907 7884 1203 Size: 1119 4585 977 1327 Fragments arranged by size: 4585 1327 1119 977 AgeI A'CCGG_T Cuts at: 6839 6839 Size: 8008 AhdI GACnn_n'nnGTC Cuts at: 6737 6737 Size: 8008 AleI CACnn'nnGTG Cuts at: 1166 3936 3989 1166 Size: 2770 53 5185 Fragments arranged by size: 5185 2770 53 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8008 AlwNI CAG_nnn'CTG Cuts at: 5751 7323 5751 Size: 1572 6436 ApaLI G'TGCA_C Cuts at: 6065 7221 6065 Size: 1156 6852 ApoI r'AATT_y Cuts at: 1493 2617 3043 3064 3800 4085 4250 4871 5317 Size: 1124 426 21 736 285 165 621 446 Cuts at: 5317 5543 5695 7804 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1124 736 621 446 426 285 226 165 152 21 AseI AT'TA_AT Cuts at: 160 1032 2901 5033 6562 160 Size: 872 1869 2132 1529 1606 Fragments arranged by size: 2132 1869 1606 1529 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8008 AvaI C'yCGr_G Cuts at: 1870 1943 2105 4884 5333 1870 Size: 73 162 2779 449 4545 Fragments arranged by size: 4545 2779 449 162 73 BamHI G'GATC_C Cuts at: 3691 5338 5705 3691 Size: 1647 367 5994 Fragments arranged by size: 5994 1647 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5329 6784 618 Size: 325 217 627 3542 1455 1842 Fragments arranged by size: 3542 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2106 2112 4069 4984 5327 1391 Size: 715 6 1957 915 343 4072 Fragments arranged by size: 4072 1957 915 715 343 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3522 3644 928 Size: 971 1623 122 5292 Fragments arranged by size: 5292 1623 971 122 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6199 6233 1903 Size: 34 4262 34 3678 Fragments arranged by size: 4262 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4172 5900 7110 4172 Size: 1728 1210 5070 Fragments arranged by size: 5070 1728 1210 BclI T'GATC_A Cuts at: 1548 2647 3697 5155 1548 Size: 1099 1050 1458 4401 Fragments arranged by size: 4401 1458 1099 1050 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6619 136 Size: 107 122 71 631 605 4947 1525 Fragments arranged by size: 4947 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8003 8003 Size: 8008 BlpI GC'TnA_GC Cuts at: 5425 5425 Size: 8008 Bme1580I G_kGCm'C Cuts at: 1790 6069 7225 1790 Size: 4279 1156 2573 Fragments arranged by size: 4279 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3650 4508 1318 Size: 2332 858 4818 Fragments arranged by size: 4818 2332 858 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5739 6697 442 Size: 416 53 83 399 24 4322 958 1753 Fragments arranged by size: 4322 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8008 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3997 3997 Size: 8008 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2586 5076 6668 1239 Size: 1347 2490 1592 2579 Fragments arranged by size: 2579 2490 1592 1347 Bpu10I CC'TnA_GC Cuts at: 4080 4080 Size: 8008 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2352 4979 6133 6998 7296 2352 Size: 2627 1154 865 298 3064 Fragments arranged by size: 3064 2627 1154 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2289 2970 4838 6671 882 Size: 617 790 681 1868 1833 2219 Fragments arranged by size: 2219 1868 1833 790 681 617 BsaAI yAC'GTr Cuts at: 493 1204 5799 493 Size: 711 4595 2702 Fragments arranged by size: 4595 2702 711 BsaBI GATnn'nnATC Cuts at: 3696 8002 3696 Size: 4306 3702 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5170 5815 6197 6832 Size: 53 83 186 936 3637 645 382 635 Cuts at: 6832 275 Size: 1451 Fragments arranged by size: 3637 1451 936 645 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6441 6839 7113 7260 7671 6441 Size: 398 274 147 411 6778 Fragments arranged by size: 6778 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2779 2809 1178 Size: 30 165 30 1376 30 6377 Fragments arranged by size: 6377 1376 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2147 3986 4967 4976 1186 Size: 958 3 1839 981 9 4218 Fragments arranged by size: 4218 1839 981 958 9 3 BseYI C'CCAG_C Cuts at: 2733 2963 7211 7707 2733 Size: 230 4248 496 3034 Fragments arranged by size: 4248 3034 496 230 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3994 5112 1163 Size: 573 2258 1118 4059 Fragments arranged by size: 4059 2258 1118 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2100 5458 6219 7247 1540 Size: 275 186 99 3358 761 1028 2301 Fragments arranged by size: 3358 2301 1028 761 275 186 99 BsiHKAI G_wGCw'C Cuts at: 2106 3996 4069 5142 6069 6154 7225 2106 Size: 1890 73 1073 927 85 1071 2889 Fragments arranged by size: 2889 1890 1073 1071 927 85 73 BsmI GAATG_Cn' Cuts at: 5519 5612 5519 Size: 93 7915 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2112 3996 4069 4984 5142 5327 Size: 399 316 6 1884 73 915 158 185 Cuts at: 5327 6069 6154 7225 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1884 1071 915 742 399 316 185 158 85 73 6 BspEI T'CCGG_A Cuts at: 7671 7671 Size: 8008 BspHI T'CATG_A Cuts at: 4332 5897 7627 4332 Size: 1565 1730 4713 Fragments arranged by size: 4713 1730 1565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4016 4019 5084 5363 844 Size: 3172 3 1065 279 3489 Fragments arranged by size: 3489 3172 1065 279 3 BsrBI CCG'CTC Cuts at: 1394 1823 5895 1394 Size: 429 4072 3507 Fragments arranged by size: 4072 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3883 6503 6677 65 Size: 1183 2635 2620 174 1396 Fragments arranged by size: 2635 2620 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 2182 6652 6839 1700 Size: 110 221 151 4470 187 2869 Fragments arranged by size: 4470 2869 221 187 151 110 BsrGI T'GTAC_A Cuts at: 96 4554 96 Size: 4458 3550 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8008 BssSI C'ACGA_G Cuts at: 3104 6068 7080 3104 Size: 2964 1012 4032 Fragments arranged by size: 4032 2964 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3133 794 Size: 2339 5669 BstBI TT'CG_AA Cuts at: 6812 6812 Size: 8008 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8008 BstYI r'GATC_y Cuts at: 1972 3691 5338 5705 6090 6107 7559 7645 7657 Size: 1719 1647 367 385 17 1452 86 12 Cuts at: 7657 8003 1972 Size: 346 1977 Fragments arranged by size: 1977 1719 1647 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 2169 3421 513 Size: 552 939 90 75 1252 5100 Fragments arranged by size: 5100 1252 939 552 90 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7269 Fragments arranged by size: 7269 683 56 BtsI GCAGTG_nn' Cuts at: 5524 6318 6338 5524 Size: 794 20 7194 Fragments arranged by size: 7194 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8008 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7973 DraI TTT'AAA Cuts at: 3900 4092 5312 3900 Size: 192 1220 6596 Fragments arranged by size: 6596 1220 192 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7015 809 Size: 507 5699 1802 Fragments arranged by size: 5699 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 5455 6344 Size: 54 1425 50 275 18 267 3358 889 Cuts at: 6344 7973 8 Size: 1629 43 Fragments arranged by size: 3358 1629 1425 889 275 267 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2097 5455 1537 Size: 275 285 3358 4090 Fragments arranged by size: 4090 3358 285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2635 2758 4045 4464 5232 5241 5938 1353 Size: 1282 123 1287 419 768 9 697 3423 Fragments arranged by size: 3423 1287 1282 768 697 419 123 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2018 2176 6595 6969 7115 387 Size: 1064 138 429 158 4419 374 146 1280 Fragments arranged by size: 4419 1280 1064 429 374 158 146 138 EcoICRI GAG'CTC Cuts at: 2104 4067 2104 Size: 1963 6045 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2586 4485 4657 5076 5154 6071 6668 Size: 393 954 1899 172 419 78 917 597 Cuts at: 6668 7455 7723 1239 Size: 787 268 1524 Fragments arranged by size: 1899 1524 954 917 787 597 419 393 268 172 78 EcoNI CCTnn'n_nnAGG Cuts at: 2891 2891 Size: 8008 EcoRI G'AATT_C Cuts at: 3043 4250 5317 5695 3043 Size: 1207 1067 378 5356 Fragments arranged by size: 5356 1207 1067 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3152 5280 3152 Size: 2128 5880 FspI TGC'GCA Cuts at: 6514 6514 Size: 8008 HaeII r_GCGC'y Cuts at: 1678 2088 7155 1678 Size: 410 5067 2531 Fragments arranged by size: 5067 2531 410 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2660 2692 2735 2767 2915 Size: 32 135 32 1088 32 43 32 148 Cuts at: 2915 2947 5140 5147 5172 5179 6649 6681 6723 Size: 32 2193 7 25 7 1470 32 42 Cuts at: 6723 6755 1373 Size: 32 2626 Fragments arranged by size: 2626 2193 1470 1088 148 135 43 42 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2374 3616 4036 4801 5352 5598 Size: 988 281 436 1242 420 765 551 246 Cuts at: 5598 669 Size: 3079 Fragments arranged by size: 3079 1242 988 765 551 436 420 281 246 HindIII A'AGCT_T Cuts at: 748 2065 4738 748 Size: 1317 2673 4018 Fragments arranged by size: 4018 2673 1317 HpaI GTT'AAC Cuts at: 5598 5598 Size: 8008 KpnI G_GTAC'C Cuts at: 5333 5333 Size: 8008 MfeI C'AATT_G Cuts at: 3180 5151 5607 3180 Size: 1971 456 5581 Fragments arranged by size: 5581 1971 456 MluI A'CGCG_T Cuts at: 7884 7884 Size: 8008 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3125 3299 7122 7306 1305 Size: 1820 174 3823 184 2007 Fragments arranged by size: 3823 2007 1820 184 174 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6186 Fragments arranged by size: 6186 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3936 3989 4947 5966 6325 6484 518 Size: 648 2770 53 958 1019 359 159 2042 Fragments arranged by size: 2770 2042 1019 958 648 359 159 53 MspA1I CmG'CkG Cuts at: 1606 2096 2171 5424 6101 7249 7494 1606 Size: 490 75 3253 677 1148 245 2120 Fragments arranged by size: 3253 2120 1148 677 490 245 75 NaeI GCC'GGC Cuts at: 2033 2033 Size: 8008 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6517 Fragments arranged by size: 6517 939 552 NdeI CA'TA_TG Cuts at: 387 3172 387 Size: 2785 5223 NgoMIV G'CCGG_C Cuts at: 2031 2031 Size: 8008 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8008 NotI GC'GGCC_GC Cuts at: 5455 5455 Size: 8008 NspI r_CATG'y Cuts at: 1132 1229 2275 2326 2423 5366 6911 1132 Size: 97 1046 51 97 2943 1545 2229 Fragments arranged by size: 2943 2229 1545 1046 97 97 51 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8008 PciI A'CATG_T Cuts at: 2322 6907 2322 Size: 4585 3423 PflMI CCAn_nnn'nTGG Cuts at: 1108 3089 4854 1108 Size: 1981 1765 4262 Fragments arranged by size: 4262 1981 1765 PmeI GTTT'AAAC Cuts at: 5312 5312 Size: 8008 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6077 6109 7590 7622 6077 Size: 32 1481 32 6463 Fragments arranged by size: 6463 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2943 1613 Size: 1330 6678 PsiI TTA'TAA Cuts at: 3663 4458 5578 3663 Size: 795 1120 6093 Fragments arranged by size: 6093 1120 795 PspXI vC'TCGA_Gb Cuts at: 2105 4884 2105 Size: 2779 5229 PstI C_TGCA'G Cuts at: 830 5360 830 Size: 4530 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8008 SacI G_AGCT'C Cuts at: 2106 4069 2106 Size: 1963 6045 SacII CC_GC'GG Cuts at: 2097 2172 2097 Size: 75 7933 SalI G'TCGA_C Cuts at: 1936 5350 1936 Size: 3414 4594 SbfI CC_TGCA'GG Cuts at: 5360 5360 Size: 8008 ScaI AGT'ACT Cuts at: 6256 7968 6256 Size: 1712 6296 SfcI C'TryA_G Cuts at: 826 1045 2424 4527 5356 6491 7172 7363 826 Size: 219 1379 2103 829 1135 681 191 1471 Fragments arranged by size: 2103 1471 1379 1135 829 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8008 SgrAI Cr'CCGG_yG Cuts at: 1700 2031 1700 Size: 331 7677 SmaI CCC'GGG Cuts at: 1872 5335 1872 Size: 3463 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2105 2367 4884 4958 6112 7013 Size: 197 926 162 262 2517 74 1154 901 Cuts at: 7013 7275 820 Size: 262 1553 Fragments arranged by size: 2517 1553 1154 926 901 262 262 197 162 74 SnaBI TAC'GTA Cuts at: 493 5799 493 Size: 5306 2702 SpeI A'CTAG_T Cuts at: 152 2768 152 Size: 2616 5392 SphI G_CATG'C Cuts at: 1132 5366 1132 Size: 4234 3774 SspI AAT'ATT Cuts at: 5 52 2853 3545 3871 5932 5 Size: 47 2801 692 326 2061 2081 Fragments arranged by size: 2801 2081 2061 692 326 47 StuI AGG'CCT Cuts at: 1517 3977 1517 Size: 2460 5548 StyI C'CwwG_G Cuts at: 513 1065 2004 2233 2941 4257 5447 513 Size: 552 939 229 708 1316 1190 3074 Fragments arranged by size: 3074 1316 1190 939 708 552 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2633 6035 6052 6205 6390 6835 325 Size: 1255 1053 3402 17 153 185 445 1498 Fragments arranged by size: 3402 1498 1255 1053 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4554 6254 7966 Size: 274 80 33 51 1443 2577 1700 1712 Cuts at: 7966 96 Size: 138 Fragments arranged by size: 2577 1712 1700 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4767 4799 7968 8000 4767 Size: 32 3169 32 4775 Fragments arranged by size: 4775 3169 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8008 XbaI T'CTAG_A Cuts at: 2858 5344 2858 Size: 2486 5522 XhoI C'TCGA_G Cuts at: 1943 2105 4884 1943 Size: 162 2779 5067 Fragments arranged by size: 5067 2779 162 XmaI C'CCGG_G Cuts at: 1870 5333 1870 Size: 3463 4545 XmnI GAAnn'nnTTC Cuts at: 2770 3726 4599 6137 2770 Size: 956 873 1538 4641 Fragments arranged by size: 4641 1538 956 873 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5816 276 Size: 53 83 186 936 4282 2468 Fragments arranged by size: 4282 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI