(Linear) (Six-base) MAPSORT of: KIEE2315.seq Check: 7497 from: 1 to: 3303 KIEE2315.orf With 182 enzymes: SgfI * January 11, 2011 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2014 3303 Size: 2014 1289 AccI GT'mk_AC Cuts at: 0 1385 3303 Size: 1385 1918 AclI AA'CG_TT Cuts at: 0 2789 3303 Size: 2789 514 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2480 2652 3149 3303 Size: 2480 172 497 154 Fragments arranged by size: 2480 497 172 154 AflIII A'CryG_T Cuts at: 0 317 3303 Size: 317 2986 AleI CACnn'nnGTG Cuts at: 0 1931 1984 3303 Size: 1931 53 1319 Fragments arranged by size: 1931 1319 53 ApoI r'AATT_y Cuts at: 0 612 1038 1059 1795 2080 2245 2866 3303 Size: 612 426 21 736 285 165 621 437 Fragments arranged by size: 736 621 612 437 426 285 165 21 AseI AT'TA_AT Cuts at: 0 896 3028 3303 Size: 896 2132 275 Fragments arranged by size: 2132 896 275 AvaI C'yCGr_G Cuts at: 0 100 2879 3303 Size: 100 2779 424 Fragments arranged by size: 2779 424 100 BamHI G'GATC_C Cuts at: 0 1686 3303 Size: 1686 1617 BanII G_rGCy'C Cuts at: 0 101 107 2064 2979 3303 Size: 101 6 1957 915 324 Fragments arranged by size: 1957 915 324 101 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 1517 1639 3303 Size: 1517 122 1664 Fragments arranged by size: 1664 1517 122 BciVI GTATCCnnnnn_n' Cuts at: 0 2167 3303 Size: 2167 1136 BclI T'GATC_A Cuts at: 0 642 1692 3150 3303 Size: 642 1050 1458 153 Fragments arranged by size: 1458 1050 642 153 BmgBI CAC'GTC Cuts at: 0 1645 2503 3303 Size: 1645 858 800 Fragments arranged by size: 1645 858 800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1992 3303 Size: 1992 1311 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 581 3071 3303 Size: 581 2490 232 Fragments arranged by size: 2490 581 232 Bpu10I CC'TnA_GC Cuts at: 0 2075 3303 Size: 2075 1228 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 347 2974 3303 Size: 347 2627 329 Fragments arranged by size: 2627 347 329 BsaI GGTCTCn'nnnn_ Cuts at: 0 284 965 2833 3303 Size: 284 681 1868 470 Fragments arranged by size: 1868 681 470 284 BsaBI GATnn'nnATC Cuts at: 0 1691 3303 Size: 1691 1612 BsaHI Gr'CG_yC Cuts at: 0 3165 3303 Size: 3165 138 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 774 804 3303 Size: 774 30 2499 Fragments arranged by size: 2499 774 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 139 142 1981 2962 2971 3303 Size: 139 3 1839 981 9 332 Fragments arranged by size: 1839 981 332 139 9 3 BseYI C'CCAG_C Cuts at: 0 728 958 3303 Size: 728 230 2345 Fragments arranged by size: 2345 728 230 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1989 3107 3303 Size: 1989 1118 196 Fragments arranged by size: 1989 1118 196 BsiEI CG_ry'CG Cuts at: 0 95 3303 Size: 95 3208 BsiHKAI G_wGCw'C Cuts at: 0 101 1991 2064 3137 3303 Size: 101 1890 73 1073 166 Fragments arranged by size: 1890 1073 166 101 73 Bsp1286I G_dGCh'C Cuts at: 0 101 107 1991 2064 2979 3137 3303 Size: 101 6 1884 73 915 158 166 Fragments arranged by size: 1884 915 166 158 101 73 6 BspHI T'CATG_A Cuts at: 0 2327 3303 Size: 2327 976 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2011 2014 3079 3303 Size: 2011 3 1065 224 Fragments arranged by size: 2011 1065 224 3 BsrDI GCAATG_nn' Cuts at: 0 1878 3303 Size: 1878 1425 BsrFI r'CCGG_y Cuts at: 0 26 177 3303 Size: 26 151 3126 Fragments arranged by size: 3126 151 26 BsrGI T'GTAC_A Cuts at: 0 2549 3303 Size: 2549 754 BssSI C'ACGA_G Cuts at: 0 1099 3303 Size: 1099 2204 BstAPI GCAn_nnn'nTGC Cuts at: 0 1128 3303 Size: 1128 2175 BstYI r'GATC_y Cuts at: 0 1686 3303 Size: 1686 1617 BtgI C'CryG_G Cuts at: 0 89 164 1416 3303 Size: 89 75 1252 1887 Fragments arranged by size: 1887 1252 89 75 DraI TTT'AAA Cuts at: 0 1895 2087 3303 Size: 1895 192 1216 Fragments arranged by size: 1895 1216 192 EaeI y'GGCC_r Cuts at: 0 92 3303 Size: 92 3211 EagI C'GGCC_G Cuts at: 0 92 3303 Size: 92 3211 EarI CTCTTCn'nnn_ Cuts at: 0 630 753 2040 2459 3227 3236 3303 Size: 630 123 1287 419 768 9 67 Fragments arranged by size: 1287 768 630 419 123 67 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 13 171 3303 Size: 13 158 3132 Fragments arranged by size: 3132 158 13 EcoICRI GAG'CTC Cuts at: 0 99 2062 3303 Size: 99 1963 1241 Fragments arranged by size: 1963 1241 99 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 581 2480 2652 3071 3149 3303 Size: 581 1899 172 419 78 154 Fragments arranged by size: 1899 581 419 172 154 78 EcoNI CCTnn'n_nnAGG Cuts at: 0 886 3303 Size: 886 2417 EcoRI G'AATT_C Cuts at: 0 1038 2245 3303 Size: 1038 1207 1058 Fragments arranged by size: 1207 1058 1038 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1147 3275 3303 Size: 1147 2128 28 Fragments arranged by size: 2128 1147 28 HaeII r_GCGC'y Cuts at: 0 83 3303 Size: 83 3220 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 655 687 730 762 910 942 3135 3142 Size: 655 32 43 32 148 32 2193 7 Cuts at: 3142 3167 3174 3303 Size: 25 7 129 Fragments arranged by size: 2193 655 148 129 43 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 0 369 1611 2031 2796 3303 Size: 369 1242 420 765 507 Fragments arranged by size: 1242 765 507 420 369 HindIII A'AGCT_T Cuts at: 0 60 2733 3303 Size: 60 2673 570 Fragments arranged by size: 2673 570 60 MfeI C'AATT_G Cuts at: 0 1175 3146 3303 Size: 1175 1971 157 Fragments arranged by size: 1971 1175 157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1120 1294 3303 Size: 1120 174 2009 Fragments arranged by size: 2009 1120 174 MslI CAynn'nnrTG Cuts at: 0 1931 1984 2942 3303 Size: 1931 53 958 361 Fragments arranged by size: 1931 958 361 53 MspA1I CmG'CkG Cuts at: 0 91 166 3303 Size: 91 75 3137 Fragments arranged by size: 3137 91 75 NaeI GCC'GGC Cuts at: 0 28 3303 Size: 28 3275 NdeI CA'TA_TG Cuts at: 0 1167 3303 Size: 1167 2136 NgoMIV G'CCGG_C Cuts at: 0 26 3303 Size: 26 3277 NspI r_CATG'y Cuts at: 0 270 321 418 3303 Size: 270 51 97 2885 Fragments arranged by size: 2885 270 97 51 PciI A'CATG_T Cuts at: 0 317 3303 Size: 317 2986 PflMI CCAn_nnn'nTGG Cuts at: 0 1084 2849 3303 Size: 1084 1765 454 Fragments arranged by size: 1765 1084 454 PshAI GACnn'nnGTC Cuts at: 0 938 3303 Size: 938 2365 PsiI TTA'TAA Cuts at: 0 1658 2453 3303 Size: 1658 795 850 Fragments arranged by size: 1658 850 795 PspXI vC'TCGA_Gb Cuts at: 0 100 2879 3303 Size: 100 2779 424 Fragments arranged by size: 2779 424 100 SacI G_AGCT'C Cuts at: 0 101 2064 3303 Size: 101 1963 1239 Fragments arranged by size: 1963 1239 101 SacII CC_GC'GG Cuts at: 0 92 167 3303 Size: 92 75 3136 Fragments arranged by size: 3136 92 75 SfcI C'TryA_G Cuts at: 0 419 2522 3303 Size: 419 2103 781 Fragments arranged by size: 2103 781 419 SgrAI Cr'CCGG_yG Cuts at: 0 26 3303 Size: 26 3277 SmlI C'TyrA_G Cuts at: 0 100 362 2879 2953 3303 Size: 100 262 2517 74 350 Fragments arranged by size: 2517 350 262 100 74 SpeI A'CTAG_T Cuts at: 0 763 3303 Size: 763 2540 SspI AAT'ATT Cuts at: 0 848 1540 1866 3303 Size: 848 692 326 1437 Fragments arranged by size: 1437 848 692 326 StuI AGG'CCT Cuts at: 0 1972 3303 Size: 1972 1331 StyI C'CwwG_G Cuts at: 0 228 936 2252 3303 Size: 228 708 1316 1051 Fragments arranged by size: 1316 1051 708 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 628 3303 Size: 628 2675 TatI w'GTAC_w Cuts at: 0 2549 3303 Size: 2549 754 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2762 2794 3303 Size: 2762 32 509 Fragments arranged by size: 2762 509 32 XbaI T'CTAG_A Cuts at: 0 853 3303 Size: 853 2450 XhoI C'TCGA_G Cuts at: 0 100 2879 3303 Size: 100 2779 424 Fragments arranged by size: 2779 424 100 XmnI GAAnn'nnTTC Cuts at: 0 765 1721 2594 3303 Size: 765 956 873 709 Fragments arranged by size: 956 873 765 709 Enzymes that do cut: AarI AccI AclI AcuI AflIII AleI ApoI AseI AvaI BamHI BanII BbsI BciVI BclI BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstYI BtgI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI HaeII Hin4I HincII HindIII MfeI MmeI MslI MspA1I NaeI NdeI NgoMIV NspI PciI PflMI PshAI PsiI PspXI SacI SacII SfcI SgrAI SmlI SpeI SspI StuI StyI TaqII TatI TstI XbaI XhoI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BsaAI BsaWI BsiWI BsmI BsmBI BspEI BsrBI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EcoO109I EcoRV FseI FspI FspAI HpaI KasI KpnI MluI MscI NarI NcoI NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SphI SrfI SwaI Tth111I XcmI XmaI ZraI