################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## Acc65I AccIII AclI AcsI AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvrII BbrPI BbsI BbvCI BciVI BcnI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI HpaI KpnI MamI MluI MroI NaeI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 109 GACGTC GACGT^C AccI 1297 GTATAC GT^[AC][GT]AC AccII 206 CGCG CG^CG AflII 688 CTTAAG C^TTAAG AocI 273 CCTGAGG CC^T[ACGT]AGG AspHI 993 GAGCAC G[AT]GC[AT]^C AviII 96 TGCGCA TGC^GCA BamHI 388 GGATCC G^GATCC BanII 156 GAGCCC G[GA]GC[CT]^C BbeI 79 GGCGCC GGCGC^C BcgI 1068 CGACACCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1512 TGATCA T^GATCA BfaI 664 CTAG C^TAG BfrI 688 CTTAAG C^TTAAG BglI 187 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseRI 409 GAGGAG GAGGAG(10/8) BsgI 145 CTGCAC GTGCAG(16/14) BsiHKAI 993 GAGCAC G[AT]GC[AT]^C BsmAI 261 GTCTC GTCTC(1/5) BspMI 93 ACCTGC ACCTGC(4/8) Bst1107I 1297 GTATAC GTA^TAC BstEII 1394 GGTTACC G^GT[ACGT]ACC BstPI 1394 GGTTACC G^GT[ACGT]ACC BstUI 206 CGCG CG^CG BstXI 1479 CCAACTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 388 GGATCC [GA]^GATC[CT] BstZ17I 1297 GTATAC GTA^TAC Bsu36I 273 CCTGAGG CC^T[ACGT]AGG DraIII 148 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 273 CCTGAGG CC^T[ACGT]AGG EcoNI 687 CCTTAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1394 GGTTACC G^GT[ACGT]ACC FauI 204 CCCGC CCCGC(4/6) FbaI 1512 TGATCA T^GATCA FspI 96 TGCGCA TGC^GCA HapII 1548 CCGG C^CGG HgaI 184 GACGC GACGC(5/10) HincII 364 GTCAAC GT[CT]^[GA]AC HindII 364 GTCAAC GT[CT]^[GA]AC HpaII 1548 CCGG C^CGG KasI 79 GGCGCC G^GCGCC KspI 205 CCGCGG CCGC^GG MaeI 664 CTAG C^TAG MaeII 110 ACGT A^CGT MflI 388 GGATCC [GA]^GATC[CT] MslI 1245 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1548 CCGG C^CGG MvnI 206 CGCG CG^CG NarI 79 GGCGCC GG^CGCC NdeI 1040 CATATG CA^TATG PflMI 1173 CCATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1079 CAGCTG CAG^CTG SacII 205 CCGCGG CCGC^GG ScaI 1385 AGTACT AGT^ACT SexAI 149 ACCTGGT A^CC[AT]GGT SfcI 506 CTGTAG C^T[GA][CT]AG SfoI 79 GGCGCC GGC^GCC StuI 1000 AGGCCT AGG^CCT Van91I 1173 CCATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1247 CCAAGGTGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 388 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BalI 194 TGGCCA TGG^CCA 248 TGGCCA BsmFI 1449 GGGAC GGGAC(10/14) 65 GTCCC BsrDI 1306 CATTGC GCAATG(2/0) 668 GCAATG DraII 63 GGGTCCC [GA]G^G[ACGT]CC[CT] 1449 GGGACCT DsaI 197 CCACGG C^C[GA][CT]GG 205 CCGCGG EcoO109I 63 GGGTCCC [GA]G^G[ACGT]CC[CT] 1449 GGGACCT HaeII 79 GGCGCC [GA]GCGC^[CT] 1053 AGCGCC HindIII 235 AAGCTT A^AGCTT 1288 AAGCTT HinfI 325 GAATC G^A[ACGT]TC 871 GATTC MfeI 445 CAATTG C^AATTG 742 CAATTG MluNI 194 TGGCCA TGG^CCA 248 TGGCCA MscI 194 TGGCCA TGG^CCA 248 TGGCCA MunI 445 CAATTG C^AATTG 742 CAATTG NlaIII 881 CATG CATG^ 1456 CATG PpuMI 63 GGGTCCC [GA]G^G[AT]CC[CT] 1449 GGGACCT SapI 837 GAAGAGC GCTCTTC(1/4) 632 GCTCTTC TfiI 325 GAATC G^A[AT]TC 871 GATTC Tsp45I 175 GTCAC ^GT[GC]AC 1378 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI Asp700I AvaII BanI BbvI BmyI BsaHI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI Hin1I HinP1I HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XmnI