(Circular) (Six-base) MAPSORT of: pFN21AE5654.seq Check: 2382 from: 1 to: 6334 pFN21AE5654 6334 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2118 4144 278 Size: 53 83 186 936 582 2026 2468 Fragments arranged by size: 2468 2026 936 582 186 83 53 AccI GT'mk_AC Cuts at: 1937 3303 3677 1937 Size: 1366 374 4594 Fragments arranged by size: 4594 1366 374 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2861 2873 4397 5781 6049 1632 Size: 1229 12 1524 1384 268 1917 Fragments arranged by size: 1917 1524 1384 1229 268 12 AflII C'TTAA_G Cuts at: 820 1017 2693 820 Size: 197 1676 4461 Fragments arranged by size: 4461 1676 197 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 3254 1166 Size: 2088 4246 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6334 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2198 2230 2198 Size: 32 6302 AlwNI CAG_nnn'CTG Cuts at: 4077 5649 4077 Size: 1572 4762 ApaLI G'TGCA_C Cuts at: 4391 5547 4391 Size: 1156 5178 ApoI r'AATT_y Cuts at: 1493 3643 3869 4021 6130 1493 Size: 2150 226 152 2109 1697 Fragments arranged by size: 2150 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 3659 1870 Size: 73 1716 4545 Fragments arranged by size: 4545 1716 73 BamHI G'GATC_C Cuts at: 2393 3664 4031 2393 Size: 1271 367 4696 Fragments arranged by size: 4696 1271 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2291 3248 3655 5110 Size: 325 217 627 297 207 957 407 1455 Cuts at: 5110 618 Size: 1842 Fragments arranged by size: 1842 1455 957 627 407 325 297 217 207 BanII G_rGCy'C Cuts at: 1391 2165 3653 1391 Size: 774 1488 4072 Fragments arranged by size: 4072 1488 774 BbeI G_GCGC'C Cuts at: 2088 2088 Size: 6334 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5363 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3062 3096 4525 4559 1903 Size: 34 50 34 1041 34 1429 34 3678 Fragments arranged by size: 3678 1429 1041 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4226 5436 4226 Size: 1210 5124 BclI T'GATC_A Cuts at: 1548 3517 1548 Size: 1969 4365 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2198 4945 136 Size: 107 122 71 631 605 526 2747 1525 Fragments arranged by size: 2747 1525 631 605 526 122 107 71 BglII A'GATC_T Cuts at: 6329 6329 Size: 6334 BlpI GC'TnA_GC Cuts at: 3751 3751 Size: 6334 Bme1580I G_kGCm'C Cuts at: 1790 3251 4395 5551 1790 Size: 1461 1144 1156 2573 Fragments arranged by size: 2573 1461 1156 1144 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3578 4065 5023 Size: 416 53 83 399 24 2161 487 958 Cuts at: 5023 442 Size: 1753 Fragments arranged by size: 2161 1753 958 487 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4994 1239 Size: 3755 2579 Bpu10I CC'TnA_GC Cuts at: 2542 2542 Size: 6334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2349 2485 4459 5324 5622 2349 Size: 136 1974 865 298 3061 Fragments arranged by size: 3061 1974 865 298 136 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4997 882 Size: 617 3498 2219 Fragments arranged by size: 3498 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4125 493 Size: 711 2921 2702 Fragments arranged by size: 2921 2702 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 2115 2190 4141 Size: 53 83 186 936 552 30 75 1951 Cuts at: 4141 4523 5158 275 Size: 382 635 1451 Fragments arranged by size: 1951 1451 936 635 552 382 186 83 75 53 30 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 1178 Size: 30 165 30 1002 30 5077 Fragments arranged by size: 5077 1002 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2429 1186 Size: 1243 5091 BseYI C'CCAG_C Cuts at: 2065 5537 6033 2065 Size: 3472 496 2366 Fragments arranged by size: 3472 2366 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 1163 Size: 573 399 5362 Fragments arranged by size: 5362 573 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3784 4545 5573 1540 Size: 275 186 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3002 4395 4480 5551 3002 Size: 1393 85 1071 3785 Fragments arranged by size: 3785 1393 1071 85 BsmI GAATG_Cn' Cuts at: 3845 3938 3845 Size: 93 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 3002 3251 3653 4395 4480 5551 Size: 399 375 837 249 402 742 85 1071 Cuts at: 5551 1391 Size: 2174 Fragments arranged by size: 2174 1071 837 742 402 399 375 249 85 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 4223 5953 4223 Size: 1730 4604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2107 3689 844 Size: 1263 1582 3489 Fragments arranged by size: 3489 1582 1263 BsrBI CCG'CTC Cuts at: 1394 1823 4221 1394 Size: 429 2398 3507 Fragments arranged by size: 3507 2398 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2680 3310 4829 5003 65 Size: 1183 1432 630 1519 174 1396 Fragments arranged by size: 1519 1432 1396 1183 630 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4978 5165 1700 Size: 110 3168 187 2869 Fragments arranged by size: 3168 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6334 BssSI C'ACGA_G Cuts at: 4394 5406 4394 Size: 1012 5322 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6334 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 3399 1184 Size: 2215 4119 BstXI CCAn_nnnn'nTGG Cuts at: 3491 3491 Size: 6334 BstYI r'GATC_y Cuts at: 1972 2393 3664 4031 4416 4433 5885 5971 5983 Size: 421 1271 367 385 17 1452 86 12 Cuts at: 5983 6329 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1271 421 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3304 3304 Size: 6334 Bsu36I CC'TnA_GG Cuts at: 2279 2279 Size: 6334 BtgI C'CryG_G Cuts at: 513 1065 2202 2210 513 Size: 552 1137 8 4637 Fragments arranged by size: 4637 1137 552 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5595 Fragments arranged by size: 5595 683 56 BtsI GCAGTG_nn' Cuts at: 2603 3850 4644 4664 2603 Size: 1247 794 20 4273 Fragments arranged by size: 4273 1247 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DraIII CAC_nnn'GTG Cuts at: 2158 2158 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2253 3554 Size: 54 1425 50 275 18 369 54 1301 Cuts at: 3554 3781 4670 6299 8 Size: 227 889 1629 43 Fragments arranged by size: 1629 1425 1301 889 369 275 227 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2337 2644 2837 3231 4264 1353 Size: 984 307 193 394 1033 3423 Fragments arranged by size: 3423 1033 984 394 307 193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3069 4921 5295 5441 387 Size: 1064 138 1480 1852 374 146 1280 Fragments arranged by size: 1852 1480 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2861 2873 4397 4994 5781 6049 1239 Size: 393 1229 12 1524 597 787 268 1524 Fragments arranged by size: 1524 1524 1229 787 597 393 268 12 EcoNI CCTnn'n_nnAGG Cuts at: 2696 2696 Size: 6334 EcoO109I rG'GnC_Cy Cuts at: 2069 3455 2069 Size: 1386 4948 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2320 2791 2320 Size: 471 5863 FspI TGC'GCA Cuts at: 2103 4840 2103 Size: 2737 3597 HaeII r_GCGC'y Cuts at: 1678 2088 3062 5481 1678 Size: 410 974 2419 2531 Fragments arranged by size: 2531 2419 974 410 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2621 2653 4975 5007 5049 Size: 32 135 32 1049 32 2322 32 42 Cuts at: 5049 5081 1373 Size: 32 2626 Fragments arranged by size: 2626 2322 1049 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2371 3678 3924 669 Size: 988 281 433 1307 246 3079 Fragments arranged by size: 3079 1307 988 433 281 246 HindIII A'AGCT_T Cuts at: 748 2240 3293 748 Size: 1492 1053 3789 Fragments arranged by size: 3789 1492 1053 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KasI G'GCGC_C Cuts at: 2084 2084 Size: 6334 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 2450 2747 3933 2450 Size: 297 1186 4851 Fragments arranged by size: 4851 1186 297 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2409 3096 3508 5448 5632 1305 Size: 1104 687 412 1940 184 2007 Fragments arranged by size: 2007 1940 1104 687 412 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2255 10 Size: 54 1425 343 369 54 4089 Fragments arranged by size: 4089 1425 369 343 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 3254 4292 4651 4810 518 Size: 648 2088 1038 359 159 2042 Fragments arranged by size: 2088 2042 1038 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2065 2149 2212 3086 3750 4427 5575 Size: 435 24 84 63 874 664 677 1148 Cuts at: 5575 5820 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 874 677 664 435 245 84 63 24 NarI GG'CG_CC Cuts at: 2085 2085 Size: 6334 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5782 NdeI CA'TA_TG Cuts at: 387 3046 387 Size: 2659 3675 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 3692 5237 1132 Size: 97 2463 1545 2229 Fragments arranged by size: 2463 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6334 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 3184 1108 Size: 2076 4258 PfoI T'CCnGG_A Cuts at: 2625 2625 Size: 6334 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2069 3455 2069 Size: 1386 4948 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2480 2512 2480 Size: 32 6302 PstI C_TGCA'G Cuts at: 830 3686 830 Size: 2856 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 3086 3086 Size: 6334 SacII CC_GC'GG Cuts at: 2213 2213 Size: 6334 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SanDI GG'GwC_CC Cuts at: 2069 2069 Size: 6334 SapI GCTCTTCn'nnn_ Cuts at: 2644 2837 2644 Size: 193 6141 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 3392 4582 6294 3392 Size: 1190 1712 3432 Fragments arranged by size: 3432 1712 1190 SexAI A'CCwGG_T Cuts at: 2154 2154 Size: 6334 SfcI C'TryA_G Cuts at: 826 1045 2511 3682 4817 5498 5689 826 Size: 219 1466 1171 1135 681 191 1471 Fragments arranged by size: 1471 1466 1171 1135 681 219 191 SfoI GGC'GCC Cuts at: 2086 2086 Size: 6334 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 3661 1872 Size: 1789 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2364 2500 2693 4438 5339 5601 Size: 197 926 421 136 193 1745 901 262 Cuts at: 5601 820 Size: 1553 Fragments arranged by size: 1745 1553 926 901 421 262 197 193 136 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3692 1132 Size: 2560 3774 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 3007 1517 Size: 1490 4844 StyI C'CwwG_G Cuts at: 513 1065 2250 2643 3252 3332 3773 513 Size: 552 1185 393 609 80 441 3074 Fragments arranged by size: 3074 1185 609 552 441 393 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 4361 4378 4531 4716 5161 325 Size: 1255 573 2208 17 153 185 445 1498 Fragments arranged by size: 2208 1498 1255 573 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3390 4580 6292 Size: 274 80 33 51 1443 1413 1190 1712 Cuts at: 6292 96 Size: 138 Fragments arranged by size: 1712 1443 1413 1190 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3123 3155 3313 3345 6294 6326 3123 Size: 32 158 32 2949 32 3131 Fragments arranged by size: 3131 2949 158 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3259 3259 Size: 6334 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6334 XmaI C'CCGG_G Cuts at: 1870 3659 1870 Size: 1789 4545 XmnI GAAnn'nnTTC Cuts at: 2480 2846 2931 4463 2480 Size: 366 85 1532 4351 Fragments arranged by size: 4351 1532 366 85 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2116 4142 276 Size: 53 83 186 936 582 2026 2468 Fragments arranged by size: 2468 2026 936 582 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SfiI SrfI SwaI