(Linear) (Six-base) MAPSORT of: KIEE5654.seq Check: 8416 from: 1 to: 1629 KIEE5654.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. AatII G_ACGT'C Cuts at: 0 113 1629 Size: 113 1516 AccI GT'mk_AC Cuts at: 0 1298 1629 Size: 1298 331 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 856 868 1629 Size: 856 12 761 Fragments arranged by size: 856 761 12 AflII C'TTAA_G Cuts at: 0 688 1629 Size: 688 941 AleI CACnn'nnGTG Cuts at: 0 1249 1629 Size: 1249 380 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 193 225 1629 Size: 193 32 1404 Fragments arranged by size: 1404 193 32 BamHI G'GATC_C Cuts at: 0 388 1629 Size: 388 1241 BanI G'GyrC_C Cuts at: 0 79 286 1243 1629 Size: 79 207 957 386 Fragments arranged by size: 957 386 207 79 BanII G_rGCy'C Cuts at: 0 160 1629 Size: 160 1469 BbeI G_GCGC'C Cuts at: 0 83 1629 Size: 83 1546 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1057 1091 1629 Size: 1057 34 538 Fragments arranged by size: 1057 538 34 BclI T'GATC_A Cuts at: 0 1512 1629 Size: 1512 117 BglI GCCn_nnn'nGGC Cuts at: 0 193 1629 Size: 193 1436 Bme1580I G_kGCm'C Cuts at: 0 1246 1629 Size: 1246 383 BmrI ACTGGGnnnn_n' Cuts at: 0 1573 1629 Size: 1573 56 Bpu10I CC'TnA_GC Cuts at: 0 537 1629 Size: 537 1092 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 480 1629 Size: 344 136 1149 Fragments arranged by size: 1149 344 136 BsaHI Gr'CG_yC Cuts at: 0 80 110 185 1629 Size: 80 30 75 1444 Fragments arranged by size: 1444 80 75 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 400 430 1629 Size: 400 30 1199 Fragments arranged by size: 1199 400 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 424 1629 Size: 424 1205 BseYI C'CCAG_C Cuts at: 0 60 1629 Size: 60 1569 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 1629 Size: 130 1499 BsiHKAI G_wGCw'C Cuts at: 0 997 1629 Size: 997 632 Bsp1286I G_dGCh'C Cuts at: 0 160 997 1246 1629 Size: 160 837 249 383 Fragments arranged by size: 837 383 249 160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 102 1629 Size: 102 1527 BsrDI GCAATG_nn' Cuts at: 0 675 1305 1629 Size: 675 630 324 Fragments arranged by size: 675 630 324 BstEII G'GTnAC_C Cuts at: 0 1394 1629 Size: 1394 235 BstXI CCAn_nnnn'nTGG Cuts at: 0 1486 1629 Size: 1486 143 BstYI r'GATC_y Cuts at: 0 388 1629 Size: 388 1241 BstZ17I GTA'TAC Cuts at: 0 1299 1629 Size: 1299 330 Bsu36I CC'TnA_GG Cuts at: 0 274 1629 Size: 274 1355 BtgI C'CryG_G Cuts at: 0 197 205 1629 Size: 197 8 1424 Fragments arranged by size: 1424 197 8 BtsI GCAGTG_nn' Cuts at: 0 598 1629 Size: 598 1031 DraIII CAC_nnn'GTG Cuts at: 0 153 1629 Size: 153 1476 EaeI y'GGCC_r Cuts at: 0 194 248 1549 1629 Size: 194 54 1301 80 Fragments arranged by size: 1301 194 80 54 EarI CTCTTCn'nnn_ Cuts at: 0 332 639 832 1226 1629 Size: 332 307 193 394 403 Fragments arranged by size: 403 394 332 307 193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1064 1629 Size: 1064 565 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 856 868 1629 Size: 856 12 761 Fragments arranged by size: 856 761 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 691 1629 Size: 691 938 EcoO109I rG'GnC_Cy Cuts at: 0 64 1450 1629 Size: 64 1386 179 Fragments arranged by size: 1386 179 64 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 315 786 1629 Size: 315 471 843 Fragments arranged by size: 843 471 315 FspI TGC'GCA Cuts at: 0 98 1629 Size: 98 1531 HaeII r_GCGC'y Cuts at: 0 83 1057 1629 Size: 83 974 572 Fragments arranged by size: 974 572 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 616 648 1629 Size: 616 32 981 Fragments arranged by size: 981 616 32 HincII GTy'rAC Cuts at: 0 366 1629 Size: 366 1263 HindIII A'AGCT_T Cuts at: 0 235 1288 1629 Size: 235 1053 341 Fragments arranged by size: 1053 341 235 KasI G'GCGC_C Cuts at: 0 79 1629 Size: 79 1550 MfeI C'AATT_G Cuts at: 0 445 742 1629 Size: 445 297 887 Fragments arranged by size: 887 445 297 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 404 1091 1503 1629 Size: 404 687 412 126 Fragments arranged by size: 687 412 404 126 MscI TGG'CCA Cuts at: 0 196 250 1629 Size: 196 54 1379 Fragments arranged by size: 1379 196 54 MslI CAynn'nnrTG Cuts at: 0 1249 1629 Size: 1249 380 MspA1I CmG'CkG Cuts at: 0 36 60 144 207 1081 1629 Size: 36 24 84 63 874 548 Fragments arranged by size: 874 548 84 63 36 24 NarI GG'CG_CC Cuts at: 0 80 1629 Size: 80 1549 NdeI CA'TA_TG Cuts at: 0 1041 1629 Size: 1041 588 PflMI CCAn_nnn'nTGG Cuts at: 0 1179 1629 Size: 1179 450 PfoI T'CCnGG_A Cuts at: 0 620 1629 Size: 620 1009 PpuMI rG'GwC_Cy Cuts at: 0 64 1450 1629 Size: 64 1386 179 Fragments arranged by size: 1386 179 64 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 475 507 1629 Size: 475 32 1122 Fragments arranged by size: 1122 475 32 PvuII CAG'CTG Cuts at: 0 1081 1629 Size: 1081 548 SacII CC_GC'GG Cuts at: 0 208 1629 Size: 208 1421 SanDI GG'GwC_CC Cuts at: 0 64 1629 Size: 64 1565 SapI GCTCTTCn'nnn_ Cuts at: 0 639 832 1629 Size: 639 193 797 Fragments arranged by size: 797 639 193 ScaI AGT'ACT Cuts at: 0 1387 1629 Size: 1387 242 SexAI A'CCwGG_T Cuts at: 0 149 1629 Size: 149 1480 SfcI C'TryA_G Cuts at: 0 506 1629 Size: 506 1123 SfoI GGC'GCC Cuts at: 0 81 1629 Size: 81 1548 SmlI C'TyrA_G Cuts at: 0 359 495 688 1629 Size: 359 136 193 941 Fragments arranged by size: 941 359 193 136 StuI AGG'CCT Cuts at: 0 1002 1629 Size: 1002 627 StyI C'CwwG_G Cuts at: 0 245 638 1247 1327 1629 Size: 245 393 609 80 302 Fragments arranged by size: 609 393 302 245 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 148 1629 Size: 148 1481 TatI w'GTAC_w Cuts at: 0 1385 1629 Size: 1385 244 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1118 1150 1308 1340 1629 Size: 1118 32 158 32 289 Fragments arranged by size: 1118 289 158 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1254 1629 Size: 1254 375 XmnI GAAnn'nnTTC Cuts at: 0 475 841 926 1629 Size: 475 366 85 703 Fragments arranged by size: 703 475 366 85 ZraI GAC'GTC Cuts at: 0 111 1629 Size: 111 1518 Enzymes that do cut: AatII AccI AcuI AflII AleI AloI BamHI BanI BanII BbeI BcgI BclI BglI Bme1580I BmrI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HindIII KasI MfeI MmeI MscI MslI MspA1I NarI NdeI PflMI PfoI PpuMI PsrI PvuII SacII SanDI SapI ScaI SexAI SfcI SfoI SmlI StuI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbsI BbvCI BciVI BfrBI BglII BlpI BmgBI BmtI BplI BpmI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FseI FspAI HpaI KpnI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI SalI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI