Restriction map for
pFN21AE3885
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
DraI DraIII DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HincII
HindII HindIII HpaI HphI KpnI
Ksp632I KspI MaeI MaeII MamI
MboII MfeI MluI MluNI MroI
MscI MseI MslI MunI MvnI
MwoI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AciI 110 GCGG CCGC(-3/-1)
AcyI 12 GGCGCC G[GA]^CG[CT]C
AlwI 244 GGATC GGATC(4/5)
AlwNI 102 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 12 GGCGCC G^G[CT][GA]CC
BbeI 12 GGCGCC GGCGC^C
BbvI 163 GCAGC GCAGC(8/12)
BcnI 49 CCCGG CC^[GC]GG
BpmI 38 CTGGAG CTGGAG(16/14)
BsaHI 12 GGCGCC G[GA]^CG[CT]C
BsmAI 119 GTCTC GTCTC(1/5)
BsmFI 47 GTCCC GGGAC(10/14)
BsmI 178 GAATGC GAATGC(1/-1)
BsrDI 205 GCAATG GCAATG(2/0)
BsrFI 15 GCCGGT [GA]^CCGG[CT]
BsrI 74 ACTGG ACTGG(1/-1)
BstF5I 237 CATCC GGATG(2/0)
BstYI 244 GGATCT [GA]^GATC[CT]
Cac8I 3 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 13 GCGC GCG^C
Cfr10I 15 GCCGGT [GA]^CCGG[CT]
CpoI 51 CGGTCCG CG^G[AT]CCG
DdeI 8 CTCAG C^T[ACGT]AG
DpnII 245 GATC ^GATC
DraII 223 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 106 GACTGCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 223 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 313 GATATC GAT^ATC
EcoT14I 147 CCTTGG C^C[AT][AT]GG
Fnu4HI 163 GCAGC GC^[ACGT]GC
FokI 237 CATCC GGATG(9/13)
HaeII 12 GGCGCC [GA]GCGC^[CT]
HaeIII 57 GGCC GG^CC
HgaI 200 GCGTC GACGC(5/10)
HhaI 13 GCGC GCG^C
Hin1I 12 GGCGCC G[GA]^CG[CT]C
HinP1I 13 GCGC G^CGC
ItaI 163 GCAGC GC^[ACGT]GC
KasI 12 GGCGCC G^GCGCC
MboI 245 GATC ^GATC
MflI 244 GGATCT [GA]^GATC[CT]
MspA1I 164 CAGCTG C[AC]G^C[GT]G
NarI 12 GGCGCC GG^CGCC
NciI 49 CCCGG CC^[GC]GG
NdeII 245 GATC ^GATC
NspI 86 GCATGT [GA]CATG^[CT]
PpuMI 223 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 164 CAGCTG CAG^CTG
RsrII 51 CGGTCCG CG^G[AT]CCG
Sau3AI 245 GATC ^GATC
SfaNI 236 GCATC GCATC(5/9)
SfcI 219 CTACAG C^T[GA][CT]AG
SfoI 12 GGCGCC GGC^GCC
SgrAI 14 CGCCGGTG C[GA]^CCGG[CT]G
StyI 147 CCTTGG C^C[AT][AT]GG
TseI 163 GCAGC G^C[AT]GC
XhoII 244 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 290 GTAC GT^AC
338 GTAC
AluI 6 AGCT AG^CT
165 AGCT
BsaJI 147 CCTTGG C^C[ACGT][ACGT]GG
240 CCCTGG
BsiYI 37 CCTGGAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
142 CCGTGCCTTGG
BslI 37 CCTGGAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
142 CCGTGCCTTGG
BstNI 37 CCTGG CC^[AT]GG
241 CCTGG
EcoRII 37 CCTGG ^CC[AT]GG
241 CCTGG
MaeIII 28 GTGAC ^GT[ACGT]AC
92 GTGAC
MnlI 283 CCTC CCTC(7/6)
210 GAGG
MvaI 37 CCTGG CC^[AT]GG
241 CCTGG
NlaIII 87 CATG CATG^
333 CATG
RsaI 290 GTAC GT^AC
338 GTAC
Tsp45I 28 GTGAC ^GT[GC]AC
92 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AvaII BssKI Cfr13I HapII HinfI
HpaII MspI NlaIV PleI Sau96I
ScrFI