(Linear) (Six-base) MAPSORT of: KIEE3885.seq  Check: 5222  from: 1  to: 345



KIEE3885.orf

 With 182 enzymes: SgfI * 

                        August 4, 2021 20:20  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     291     345 
   Size:        291      54
 
AlwNI CAG_nnn'CTG

Cuts at:      0     107     345 
   Size:        107     238
 
BanI G'GyrC_C

Cuts at:      0      12     345 
   Size:         12     333
 
BbeI G_GCGC'C

Cuts at:      0      16     345 
   Size:         16     329
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     136     345 
   Size:        136     209
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     345 
   Size:         59     286
 
Bpu10I CC'TnA_GC

Cuts at:      0       8     345 
   Size:          8     337
 
BsaHI Gr'CG_yC

Cuts at:      0      13     345 
   Size:         13     332
 
BsmI GAATG_Cn'

Cuts at:      0     184     345 
   Size:        184     161
 
BsrDI GCAATG_nn'

Cuts at:      0     212     345 
   Size:        212     133
 
BsrFI r'CCGG_y

Cuts at:      0      15     345 
   Size:         15     330
 
BstYI r'GATC_y

Cuts at:      0     244     345 
   Size:        244     101
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     112     345 
   Size:        112     233
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     345 
   Size:         59     286
 
EcoO109I rG'GnC_Cy

Cuts at:      0     224     345 
   Size:        224     121
 
EcoRV GAT'ATC

Cuts at:      0     315     345 
   Size:        315      30
 
HaeII r_GCGC'y

Cuts at:      0      16     345 
   Size:         16     329
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     221     253     345 
   Size:        221      32      92

  Fragments arranged by size: 

                 221      92      32
 
KasI G'GCGC_C

Cuts at:      0      12     345 
   Size:         12     333
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     345 
   Size:         95     250
 
MspA1I CmG'CkG

Cuts at:      0     166     345 
   Size:        166     179
 
NarI GG'CG_CC

Cuts at:      0      13     345 
   Size:         13     332
 
NspI r_CATG'y

Cuts at:      0      90     345 
   Size:         90     255
 
PpuMI rG'GwC_Cy

Cuts at:      0     224     345 
   Size:        224     121
 
PvuII CAG'CTG

Cuts at:      0     166     345 
   Size:        166     179
 
RsrII CG'GwC_CG

Cuts at:      0      52     345 
   Size:         52     293
 
SfcI C'TryA_G

Cuts at:      0     219     345 
   Size:        219     126
 
SfoI GGC'GCC

Cuts at:      0      14     345 
   Size:         14     331
 
SgrAI Cr'CCGG_yG

Cuts at:      0      15     345 
   Size:         15     330
 
StyI C'CwwG_G

Cuts at:      0     147     345 
   Size:        147     198
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      34     140     345 
   Size:         34     106     205

  Fragments arranged by size: 

                 205     106      34
 
TatI w'GTAC_w

Cuts at:      0     289     345 
   Size:        289      56
 

 Enzymes that do cut:

     AlfI    AlwNI     BanI     BbeI     BplI     BpmI   Bpu10I    BsaHI
     BsmI    BsrDI    BsrFI    BstYI     DrdI  Eco57MI EcoO109I    EcoRV
    HaeII    Hin4I     KasI     MmeI   MspA1I     NarI     NspI    PpuMI
    PvuII    RsrII     SfcI     SfoI    SgrAI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI