Restriction map for
pFN21AE3203
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BamHI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoRI EcoT22I FauI FbaI
FseI FspI HpaI KpnI KspI
MamI MfeI MflI MluI MroI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 274 CGCG CG^CG
Aor51HI 863 AGCGCT AGC^GCT
AspHI 91 GAGCTC G[AT]GC[AT]^C
AspI 160 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 257 GGCGCC G^G[CT][GA]CC
BbeI 257 GGCGCC GGCGC^C
BcgI 11 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 415 CTAG C^TAG
BsaWI 785 ACCGGA [AT]^CCGG[AT]
BsiHKAI 91 GAGCTC G[AT]GC[AT]^C
BspMI 124 GCAGGT ACCTGC(4/8)
BsrBI 850 GAGCGG CCGCTC(-3/-3)
Bst1107I 1378 GTATAC GTA^TAC
BstUI 274 CGCG CG^CG
BstZ17I 1378 GTATAC GTA^TAC
DpnII 490 GATC ^GATC
DrdI 1392 GACAGTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 863 AGCGCT AGC^GCT
EcoRV 601 GATATC GAT^ATC
HincII 530 GTCAAC GT[CT]^[GA]AC
HindII 530 GTCAAC GT[CT]^[GA]AC
HindIII 771 AAGCTT A^AGCTT
HphI 112 GGTGA GGTGA(8/7)
KasI 257 GGCGCC G^GCGCC
MaeI 415 CTAG C^TAG
MaeII 664 ACGT A^CGT
MboI 490 GATC ^GATC
MspA1I 679 CAGCTG C[AC]G^C[GT]G
MvnI 274 CGCG CG^CG
NarI 257 GGCGCC GG^CGCC
NdeII 490 GATC ^GATC
PflFI 160 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 439 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 679 CAGCTG CAG^CTG
SacI 91 GAGCTC GAGCT^C
SapI 1032 GAAGAGC GCTCTTC(1/4)
Sau3AI 490 GATC ^GATC
SfoI 257 GGCGCC GGC^GCC
SphI 1204 GCATGC GCATG^C
TaqI 573 TCGA T^CGA
TfiI 1293 GATTC G^A[AT]TC
Tsp45I 113 GTGAC ^GT[GC]AC
Tth111I 160 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 573 TCGA T^CGA
Van91I 439 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1129 GTAGAC GT^[AC][GT]AC
1378 GTATAC
AcsI 511 AAATTC [GA]^AATT[CT]
789 GAATTT
AcyI 257 GGCGCC G[GA]^CG[CT]C
674 GACGCC
AfaI 198 GTAC GT^AC
1312 GTAC
AlwNI 440 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1060 CAGACCCTG
ApoI 511 AAATTC [GA]^AATT[CT]
789 GAATTT
AvaII 207 GGTCC G^G[AT]CC
503 GGACC
BalI 540 TGGCCA TGG^CCA
560 TGGCCA
BanII 91 GAGCTC G[GA]GC[CT]^C
228 GGGCTC
BmyI 91 GAGCTC G[AGT]GC[ACT]^C
228 GGGCTC
BsaHI 257 GGCGCC G[GA]^CG[CT]C
674 GACGCC
BsaI 69 GAGACC GGTCTC(1/5)
165 GGTCTC
BseRI 690 GAGGAG GAGGAG(10/8)
946 GAGGAG
BsmAI 69 GAGAC GTCTC(1/5)
166 GTCTC
BsmFI 672 GGGAC GGGAC(10/14)
780 GGGAC
Bsp1286I 91 GAGCTC G[AGT]GC[ACT]^C
228 GGGCTC
BstEII 112 GGTGACC G^GT[ACGT]ACC
695 GGTTACC
BstPI 112 GGTGACC G^GT[ACGT]ACC
695 GGTTACC
DraII 980 AGGCCCC [GA]G^G[ACGT]CC[CT]
1020 GGGGCCT
EaeI 540 TGGCCA [CT]^GGCC[GA]
560 TGGCCA
EarI 637 GAAGAG CTCTTC(1/4)
1032 GAAGAG
EcoNI 54 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1065 CCTGAAAAAGG
EcoO109I 980 AGGCCCC [GA]G^G[ACGT]CC[CT]
1020 GGGGCCT
EcoO65I 112 GGTGACC G^GT[ACGT]ACC
695 GGTTACC
EcoT14I 537 CCTTGG C^C[AT][AT]GG
668 CCAAGG
HaeII 257 GGCGCC [GA]GCGC^[CT]
863 AGCGCT
HgaI 674 GACGC GACGC(5/10)
1041 GACGC
Hin1I 257 GGCGCC G[GA]^CG[CT]C
674 GACGCC
Ksp632I 637 GAAGAG CTCTTC(1/4)
1032 GAAGAG
MaeIII 113 GTGAC ^GT[ACGT]AC
696 GTTAC
MluNI 540 TGGCCA TGG^CCA
560 TGGCCA
MscI 540 TGGCCA TGG^CCA
560 TGGCCA
MseI 656 TTAA T^TAA
1373 TTAA
NspI 176 ACATGC [GA]CATG^[CT]
1204 GCATGC
PleI 139 GACTC GAGTC(4/5)
1399 GAGTC
PstI 148 CTGCAG CTGCA^G
889 CTGCAG
RsaI 198 GTAC GT^AC
1312 GTAC
SfcI 148 CTGCAG C^T[GA][CT]AG
889 CTGCAG
SmlI 615 CTTGAG C^T[CT][GA]AG
1123 CTCAAG
StyI 537 CCTTGG C^C[AT][AT]GG
668 CCAAGG
Tru9I 656 TTAA T^TAA
1373 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BbvI BcnI BpmI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SfaNI TseI Tsp509I TspRI