Restriction map for
pFN21AE3203
(Circular) (Six-base) MAPSORT of: pFN21AE3203.seq Check: 1061 from: 1 to: 6121
pFN21AE3203 6121 bp
With 182 enzymes: SgfI *
March 28, 2012 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2120 2120
Size: 6121
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3135 3384 3464 1937
Size: 1198 249 80 4594
Fragments arranged by size:
4594 1198 249 80
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2960 4184 5568 5836 1632
Size: 1328 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1328 1224 268
AfeI AGC'GCT
Cuts at: 2870 2870
Size: 6121
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 5020 5997 1203
Size: 3817 977 1327
Fragments arranged by size:
3817 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2126 1184
Size: 942 5179
AlwNI CAG_nnn'CTG
Cuts at: 2450 3070 3864 5436 2450
Size: 620 794 1572 3135
Fragments arranged by size:
3135 1572 794 620
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 2516 2794 3430 3656 3808 5917 1493
Size: 1023 278 636 226 152 2109 1697
Fragments arranged by size:
2109 1697 1023 636 278 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2262 3442 4897 618
Size: 325 217 627 475 1180 1455 1842
Fragments arranged by size:
1842 1455 1180 627 475 325 217
BanII G_rGCy'C
Cuts at: 1391 2100 2237 3440 1391
Size: 709 137 1203 4072
Fragments arranged by size:
4072 1203 709 137
BbeI G_GCGC'C
Cuts at: 2266 2266
Size: 6121
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2005 2039 4312 4346 1903
Size: 34 68 34 2273 34 3678
Fragments arranged by size:
3678 2273 68 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6116 6116
Size: 6121
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 4182 5338 1790
Size: 2392 1156 2573
Fragments arranged by size:
2573 2392 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2886 3852 4810
Size: 416 53 83 399 24 1469 966 958
Cuts at: 4810 442
Size: 1753
Fragments arranged by size:
1753 1469 966 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2472 3175 3225 4781 1239
Size: 1022 211 703 50 1556 2579
Fragments arranged by size:
2579 1556 1022 703 211 50
Bpu10I CC'TnA_GC
Cuts at: 2982 3303 2982
Size: 321 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2641 3113 4246 5111 5409 2641
Size: 472 1133 865 298 3353
Fragments arranged by size:
3353 1133 865 472 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2068 2176 4784 882
Size: 617 569 108 2608 2219
Fragments arranged by size:
2608 2219 617 569 108
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2263 2680 3928 4310
Size: 53 83 186 936 730 417 1248 382
Cuts at: 4310 4945 275
Size: 635 1451
Fragments arranged by size:
1451 1248 936 730 635 417 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2790 4554 4952 5226 5373 5784 2790
Size: 1764 398 274 147 411 3127
Fragments arranged by size:
3127 1764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5896
Fragments arranged by size:
5896 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2710 2966 1186
Size: 1524 256 4341
Fragments arranged by size:
4341 1524 256
BseYI C'CCAG_C
Cuts at: 2009 2038 5324 5820 2009
Size: 29 3286 496 2310
Fragments arranged by size:
3286 2310 496 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5548
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 4182 4267 5338 2100
Size: 2082 85 1071 2883
Fragments arranged by size:
2883 2082 1071 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2237 3440 4182 4267 5338 1391
Size: 399 310 137 1203 742 85 1071 2174
Fragments arranged by size:
2174 1203 1071 742 399 310 137 85
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2120 3476 844
Size: 1276 1356 3489
Fragments arranged by size:
3489 1356 1276
BsrBI CCG'CTC
Cuts at: 1394 1823 2857 4008 1394
Size: 429 1034 1151 3507
Fragments arranged by size:
3507 1151 1034 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4616 4790 65
Size: 1183 3368 174 1396
Fragments arranged by size:
3368 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6121
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 2117 2700 1184
Size: 933 583 4605
Fragments arranged by size:
4605 933 583
BstYI r'GATC_y
Cuts at: 1972 3451 3818 4203 4220 5672 5758 5770 6116
Size: 1479 367 385 17 1452 86 12 346
Cuts at: 6116 1972
Size: 1977
Fragments arranged by size:
1977 1479 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3385 3385
Size: 6121
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4630
Fragments arranged by size:
4630 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2346 487
Size: 683 56 1120 4262
Fragments arranged by size:
4262 1120 683 56
BtsI GCAGTG_nn'
Cuts at: 3098 3637 4431 4451 3098
Size: 539 794 20 4768
Fragments arranged by size:
4768 794 539 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3403 5128 809
Size: 507 2087 1725 1802
Fragments arranged by size:
2087 1802 1725 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2545 2565 3568
Size: 54 1425 50 275 18 715 20 1003
Cuts at: 3568 4457 6086 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1003 889 715 275 54 50
43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2637 3032 4051 1353
Size: 1284 395 1019 3423
Fragments arranged by size:
3423 1284 1019 395
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2098 2098
Size: 6121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2472 2960 3175 3225 4184 4781
Size: 393 629 211 488 215 50 959 597
Cuts at: 4781 5568 5836 1239
Size: 787 268 1524
Fragments arranged by size:
1524 959 787 629 597 488 393 268
215 211 50
EcoNI CCTnn'n_nnAGG
Cuts at: 2063 3074 2063
Size: 1011 5110
EcoO109I rG'GnC_Cy
Cuts at: 2986 3026 2986
Size: 40 6081
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
EcoRV GAT'ATC
Cuts at: 2608 2608
Size: 6121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3154 3154
Size: 6121
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 2266 2872 5268 1678
Size: 588 606 2396 2531
Fragments arranged by size:
2531 2396 606 588
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 2331 2363 2573
Size: 32 135 32 584 32 143 32 210
Cuts at: 2573 2605 3088 3120 4762 4794 4836 4868 1373
Size: 32 483 32 1642 32 42 32 2626
Fragments arranged by size:
2626 1642 584 483 210 143 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2537 3465 3711 669
Size: 988 281 599 928 246 3079
Fragments arranged by size:
3079 988 928 599 281 246
HindIII A'AGCT_T
Cuts at: 748 2776 748
Size: 2028 4093
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KasI G'GCGC_C
Cuts at: 2262 2262
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5235 5419 1305
Size: 3930 184 2007
Fragments arranged by size:
3930 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2547 2567 10
Size: 54 1425 343 715 20 3564
Fragments arranged by size:
3564 1425 715 343 54 20
MslI CAynn'nnrTG
Cuts at: 518 1166 4079 4438 4597 518
Size: 648 2913 359 159 2042
Fragments arranged by size:
2913 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2686 3537 4214 5362 5607 1606
Size: 1080 851 677 1148 245 2120
Fragments arranged by size:
2120 1148 1080 851 677 245
NarI GG'CG_CC
Cuts at: 2263 2263
Size: 6121
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4630
Fragments arranged by size:
4630 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 2185 3213 3479 5024 1132
Size: 97 956 1028 266 1545 2229
Fragments arranged by size:
2229 1545 1028 956 266 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2450 1108
Size: 1342 4779
PfoI T'CCnGG_A
Cuts at: 2091 2091
Size: 6121
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PstI C_TGCA'G
Cuts at: 830 2157 2898 3473 830
Size: 1327 741 575 3478
Fragments arranged by size:
3478 1327 741 575
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 2686 2686
Size: 6121
SacI G_AGCT'C
Cuts at: 2100 2100
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SapI GCTCTTCn'nnn_
Cuts at: 3032 3032
Size: 6121
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2153 2894 3469 4604 5285 5476 826
Size: 219 1108 741 575 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 741 681 575 219 191
SfoI GGC'GCC
Cuts at: 2264 2264
Size: 6121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2620 3128 4225 5126 5388 820
Size: 197 926 677 508 1097 901 262 1553
Fragments arranged by size:
1553 1097 926 901 677 508 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3213 3479 1132
Size: 2081 266 3774
Fragments arranged by size:
3774 2081 266
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6121
StyI C'CwwG_G
Cuts at: 513 1065 2004 2542 2673 3560 513
Size: 552 939 538 131 887 3074
Fragments arranged by size:
3074 939 887 552 538 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 4148 4165 4318 4503 4948 325
Size: 1255 512 2056 17 153 185 445 1498
Fragments arranged by size:
2056 1498 1255 512 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4367 6079 96
Size: 274 80 33 51 1443 2390 1712 138
Fragments arranged by size:
2390 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2930 2962 6081 6113 2930
Size: 32 3119 32 2938
Fragments arranged by size:
3119 2938 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2168 1316
Size: 852 5269
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 4250 4250
Size: 6121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI Bsu36I DraIII FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI