Restriction map for
pFN21AE3203
(Linear) (Six-base) MAPSORT of: KIEE3203.seq Check: 8124 from: 1 to: 1416
KIEE3203.orf
With 182 enzymes: SgfI *
March 22, 2012 16:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 115 1416
Size: 115 1301
AccI GT'mk_AC
Cuts at: 0 1130 1379 1416
Size: 1130 249 37
Fragments arranged by size:
1130 249 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1416
Size: 955 461
AfeI AGC'GCT
Cuts at: 0 865 1416
Size: 865 551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 121 1416
Size: 121 1295
AlwNI CAG_nnn'CTG
Cuts at: 0 445 1065 1416
Size: 445 620 351
Fragments arranged by size:
620 445 351
ApoI r'AATT_y
Cuts at: 0 511 789 1416
Size: 511 278 627
Fragments arranged by size:
627 511 278
BanI G'GyrC_C
Cuts at: 0 257 1416
Size: 257 1159
BanII G_rGCy'C
Cuts at: 0 95 232 1416
Size: 95 137 1184
Fragments arranged by size:
1184 137 95
BbeI G_GCGC'C
Cuts at: 0 261 1416
Size: 261 1155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 1416
Size: 34 1382
BmrI ACTGGGnnnn_n'
Cuts at: 0 881 1416
Size: 881 535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 261 1416
Size: 261 1155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 467 1170 1220 1416
Size: 256 211 703 50 196
Fragments arranged by size:
703 256 211 196 50
Bpu10I CC'TnA_GC
Cuts at: 0 977 1298 1416
Size: 977 321 118
Fragments arranged by size:
977 321 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 1108 1416
Size: 636 472 308
Fragments arranged by size:
636 472 308
BsaI GGTCTCn'nnnn_
Cuts at: 0 63 171 1416
Size: 63 108 1245
Fragments arranged by size:
1245 108 63
BsaHI Gr'CG_yC
Cuts at: 0 258 675 1416
Size: 258 417 741
Fragments arranged by size:
741 417 258
BsaWI w'CCGG_w
Cuts at: 0 785 1416
Size: 785 631
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 705 961 1416
Size: 705 256 455
Fragments arranged by size:
705 455 256
BseYI C'CCAG_C
Cuts at: 0 4 33 1416
Size: 4 29 1383
Fragments arranged by size:
1383 29 4
BsiHKAI G_wGCw'C
Cuts at: 0 95 1416
Size: 95 1321
Bsp1286I G_dGCh'C
Cuts at: 0 95 232 1416
Size: 95 137 1184
Fragments arranged by size:
1184 137 95
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 115 1416
Size: 115 1301
BsrBI CCG'CTC
Cuts at: 0 852 1416
Size: 852 564
BstEII G'GTnAC_C
Cuts at: 0 112 695 1416
Size: 112 583 721
Fragments arranged by size:
721 583 112
BstZ17I GTA'TAC
Cuts at: 0 1380 1416
Size: 1380 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 341 1416
Size: 341 1075
BtsI GCAGTG_nn'
Cuts at: 0 1093 1416
Size: 1093 323
DrdI GACnn_nn'nnGTC
Cuts at: 0 1398 1416
Size: 1398 18
EaeI y'GGCC_r
Cuts at: 0 540 560 1416
Size: 540 20 856
Fragments arranged by size:
856 540 20
EarI CTCTTCn'nnn_
Cuts at: 0 632 1027 1416
Size: 632 395 389
Fragments arranged by size:
632 395 389
EcoICRI GAG'CTC
Cuts at: 0 93 1416
Size: 93 1323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 467 955 1170 1220 1416
Size: 256 211 488 215 50 196
Fragments arranged by size:
488 256 215 211 196 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 58 1069 1416
Size: 58 1011 347
Fragments arranged by size:
1011 347 58
EcoO109I rG'GnC_Cy
Cuts at: 0 981 1021 1416
Size: 981 40 395
Fragments arranged by size:
981 395 40
EcoRV GAT'ATC
Cuts at: 0 603 1416
Size: 603 813
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1149 1416
Size: 1149 267
HaeII r_GCGC'y
Cuts at: 0 261 867 1416
Size: 261 606 549
Fragments arranged by size:
606 549 261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 326 358 568 600 1083 1115
Size: 151 32 143 32 210 32 483 32
Cuts at: 1115 1416
Size: 301
Fragments arranged by size:
483 301 210 151 143 32 32 32
32
HincII GTy'rAC
Cuts at: 0 532 1416
Size: 532 884
HindIII A'AGCT_T
Cuts at: 0 771 1416
Size: 771 645
KasI G'GCGC_C
Cuts at: 0 257 1416
Size: 257 1159
MscI TGG'CCA
Cuts at: 0 542 562 1416
Size: 542 20 854
Fragments arranged by size:
854 542 20
MspA1I CmG'CkG
Cuts at: 0 681 1416
Size: 681 735
NarI GG'CG_CC
Cuts at: 0 258 1416
Size: 258 1158
NspI r_CATG'y
Cuts at: 0 180 1208 1416
Size: 180 1028 208
Fragments arranged by size:
1028 208 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 445 1416
Size: 445 971
PfoI T'CCnGG_A
Cuts at: 0 86 1416
Size: 86 1330
PstI C_TGCA'G
Cuts at: 0 152 893 1416
Size: 152 741 523
Fragments arranged by size:
741 523 152
PvuII CAG'CTG
Cuts at: 0 681 1416
Size: 681 735
SacI G_AGCT'C
Cuts at: 0 95 1416
Size: 95 1321
SapI GCTCTTCn'nnn_
Cuts at: 0 1027 1416
Size: 1027 389
SfcI C'TryA_G
Cuts at: 0 148 889 1416
Size: 148 741 527
Fragments arranged by size:
741 527 148
SfoI GGC'GCC
Cuts at: 0 259 1416
Size: 259 1157
SmlI C'TyrA_G
Cuts at: 0 615 1123 1416
Size: 615 508 293
Fragments arranged by size:
615 508 293
SphI G_CATG'C
Cuts at: 0 1208 1416
Size: 1208 208
StyI C'CwwG_G
Cuts at: 0 537 668 1416
Size: 537 131 748
Fragments arranged by size:
748 537 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 1416
Size: 87 1329
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 925 957 1416
Size: 925 32 459
Fragments arranged by size:
925 459 32
Tth111I GACn'n_nGTC
Cuts at: 0 163 1416
Size: 163 1253
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AlwNI ApoI BanI
BanII BbeI BcgI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BstEII BstZ17I BtgZI BtsI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII HindIII KasI MscI MspA1I NarI NspI PflMI
PfoI PstI PvuII SacI SapI SfcI SfoI SmlI
SphI StyI TaqII TstI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BsaAI BsaBI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI ClaI CspCI DraI DraIII EagI EciI
EcoRI FseI FspI FspAI HpaI KpnI MfeI MluI
MmeI MslI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TatI XbaI XcmI XhoI XmaI XmnI ZraI