Restriction map for
pFN21AE1807
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AclI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI BstAPI BstEII BstPI
BstXI Bsu36I CelII ClaI CpoI
DraI DraII DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeII MfeI MluI
MslI MunI NarI NciI NcoI
NheI NlaIV NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 403 GTATAC GT^[AC][GT]AC
AccII 315 CGCG CG^CG
AccIII 455 TCCGGA T^CCGGA
AcsI 586 AAATTC [GA]^AATT[CT]
AlwI 597 GATCC GGATC(4/5)
ApoI 586 AAATTC [GA]^AATT[CT]
Asp700I 73 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 904 GCAATTAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 915 GGATAC GTATCC(6/5)
BfaI 259 CTAG C^TAG
BmyI 570 GGGCAC G[AGT]GC[ACT]^C
BpmI 923 CTGGAG CTGGAG(16/14)
BsaJI 24 CCGAGG C^C[ACGT][ACGT]GG
BsaWI 455 TCCGGA [AT]^CCGG[AT]
BseAI 455 TCCGGA T^CCGGA
BsmAI 926 GAGAC GTCTC(1/5)
BsmI 128 GCATTC GAATGC(1/-1)
Bsp1286I 570 GGGCAC G[AGT]GC[ACT]^C
BspEI 455 TCCGGA T^CCGGA
BsrFI 750 GCCGGC [GA]^CCGG[CT]
BssKI 356 CCAGG ^CC[ACGT]GG
Bst1107I 403 GTATAC GTA^TAC
BstBI 860 TTCGAA TT^CGAA
BstNI 356 CCAGG CC^[AT]GG
BstUI 315 CGCG CG^CG
BstYI 596 AGATCC [GA]^GATC[CT]
BstZ17I 403 GTATAC GTA^TAC
CfoI 314 GCGC GCG^C
Cfr10I 750 GCCGGC [GA]^CCGG[CT]
Cfr13I 255 GGCCC G^G[ACGT]CC
DrdI 135 GACTGAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 356 CCAGG ^CC[AT]GG
EcoRV 379 GATATC GAT^ATC
FauI 316 GCGGG CCCGC(4/6)
HhaI 314 GCGC GCG^C
HinP1I 314 GCGC G^CGC
HincII 468 GTTGAC GT[CT]^[GA]AC
HindII 468 GTTGAC GT[CT]^[GA]AC
MaeI 259 CTAG C^TAG
MflI 596 AGATCC [GA]^GATC[CT]
MroI 455 TCCGGA T^CCGGA
MvaI 356 CCAGG CC^[AT]GG
MvnI 315 CGCG CG^CG
MwoI 754 GCCTTTTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 750 GCCGGC GCC^GGC
NdeI 188 CATATG CA^TATG
NgoMIV 750 GCCGGC G^CCGGC
NspV 860 TTCGAA TT^CGAA
PvuII 160 CAGCTG CAG^CTG
Sau96I 255 GGCCC G^G[ACGT]CC
ScrFI 356 CCAGG CC^[ACGT]GG
SfuI 860 TTCGAA TT^CGAA
SmlI 386 CTCAAG C^T[CT][GA]AG
TfiI 540 GATTC G^A[AT]TC
XhoII 596 AGATCC [GA]^GATC[CT]
XmnI 73 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 156 GCGG CCGC(-3/-1)
316 GCGG
BbsI 580 GAAGAC GAAGAC(2/6)
709 GAAGAC
BpuAI 580 GAAGAC GAAGAC(2/6)
709 GAAGAC
BsaBI 339 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
511 GATGAGCATC
BsiYI 57 CCCATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
819 CCGAATAATGG
BslI 57 CCCATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
819 CCGAATAATGG
Cac8I 365 GCAGGC GC[ACGT]^[ACGT]GC
750 GCCGGC
DdeI 137 CTGAG C^T[ACGT]AG
593 CTGAG
EarI 411 GAAGAG CTCTTC(1/4)
488 GAAGAG
HapII 456 CCGG C^CGG
751 CCGG
HpaII 456 CCGG C^CGG
751 CCGG
Ksp632I 411 GAAGAG CTCTTC(1/4)
488 GAAGAG
MaeIII 262 GTGAC ^GT[ACGT]AC
449 GTGAC
MamI 339 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
511 GATGAGCATC
MspA1I 154 CAGCGG C[AC]G^C[GT]G
160 CAGCTG
MspI 456 CCGG C^CGG
751 CCGG
NlaIII 249 CATG CATG^
306 CATG
PleI 264 GACTC GAGTC(4/5)
414 GAGTC
TaqI 777 TCGA T^CGA
861 TCGA
Tsp45I 262 GTGAC ^GT[GC]AC
449 GTGAC
TspRI 62 CAGTG CA[GC]TG[ACGT][ACGT]^
591 CACTG
TthHB8I 777 TCGA T^CGA
861 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BalI BbvI BstF5I DpnII
EaeI Fnu4HI FokI HaeIII HinfI
HphI ItaI MboI MboII MluNI
MnlI MscI MseI NdeII Sau3AI
SfaNI Tru9I TseI Tsp509I