################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII AccI AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII Hin1I HindIII HpaI HpaII KasI Ksp632I KspI MaeII MfeI MflI MluI MroI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TspRI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 227 GGTACC G^GTACC AccII 443 CGCG CG^CG AcsI 241 GAATTT [GA]^AATT[CT] AlwI 1033 GATCC GGATC(4/5) ApoI 241 GAATTT [GA]^AATT[CT] Asp700I 926 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 227 GGTACC G^GTACC BalI 89 TGGCCA TGG^CCA BanI 227 GGTACC G^G[CT][GA]CC BciVI 1020 GGATAC GTATCC(6/5) BclI 624 TGATCA T^GATCA BlpI 835 GCTGAGC GC^T[ACGT]AGC Bpu1102I 835 GCTGAGC GC^T[ACGT]AGC BsaBI 619 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmFI 1291 GGGAC GGGAC(10/14) BstUI 443 CGCG CG^CG Cac8I 1123 GCCAGC GC[ACGT]^[ACGT]GC CelII 835 GCTGAGC GC^T[ACGT]AGC CfoI 442 GCGC GCG^C DraI 1060 TTTAAA TTT^AAA DraII 1422 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 89 TGGCCA [CT]^GGCC[GA] EcoO109I 1422 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 667 CCAAGG C^C[AT][AT]GG FbaI 624 TGATCA T^GATCA HgaI 752 GACGC GACGC(5/10) HhaI 442 GCGC GCG^C HinP1I 442 GCGC G^CGC HincII 247 GTTGAC GT[CT]^[GA]AC HindII 247 GTTGAC GT[CT]^[GA]AC KpnI 227 GGTACC GGTAC^C MamI 619 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 89 TGGCCA TGG^CCA MscI 89 TGGCCA TGG^CCA MslI 1398 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1125 CAGCTG C[AC]G^C[GT]G MvnI 443 CGCG CG^CG PpuMI 1422 AGGACCT [GA]G^G[AT]CC[CT] PvuII 1125 CAGCTG CAG^CTG StyI 667 CCAAGG C^C[AT][AT]GG XcmI 560 CCATTGGTTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 926 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwNI 455 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1445 CAGGGGCTG AvaII 1206 GGACC G^G[AT]CC 1423 GGACC BbvI 454 GCAGC GCAGC(8/12) 1190 GCAGC BfaI 232 CTAG C^TAG 292 CTAG BsiYI 18 CCTGGGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1306 CCAATTCAGGG BslI 18 CCTGGGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1306 CCAATTCAGGG BsrDI 142 GCAATG GCAATG(2/0) 1193 GCAATG BsrI 76 ACTGG ACTGG(1/-1) 405 CCAGT BstF5I 867 GGATG GGATG(2/0) 1326 GGATG DpnII 625 GATC ^GATC 1033 GATC Fnu4HI 454 GCAGC GC^[ACGT]GC 1190 GCAGC FokI 867 GGATG GGATG(9/13) 1326 GGATG HaeIII 90 GGCC GG^CC 558 GGCC ItaI 454 GCAGC GC^[ACGT]GC 1190 GCAGC MaeI 232 CTAG C^TAG 292 CTAG MboI 625 GATC ^GATC 1033 GATC MboII 80 GAAGA GAAGA(8/7) 994 GAAGA NdeII 625 GATC ^GATC 1033 GATC NlaIV 227 GGTACC GG[ACGT]^[ACGT]CC 594 GGAACC PstI 1188 CTGCAG CTGCA^G 1259 CTGCAG Sau3AI 625 GATC ^GATC 1033 GATC SfaNI 919 GATGC GCATC(5/9) 672 GCATC TseI 454 GCAGC G^C[AT]GC 1190 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AluI AseI AsnI AspHI BanII BmyI BpmI BsaJI BsiHKAI BsmAI Bsp1286I BssKI BstNI Cfr13I DdeI EcoRII HinfI HphI MaeIII MnlI MseI MvaI MwoI NlaIII PshBI RsaI SacI Sau96I ScrFI SfcI TfiI Tru9I Tsp45I Tsp509I