(Circular) (Six-base) MAPSORT of: pFN21AE3422.seq Check: 133 from: 1 to: 6184 pFN21AE3422 6184 bp With 182 enzymes: SgfI * March 28, 2012 12:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3527 1937 Size: 1590 4594 Acc65I G'GTAC_C Cuts at: 2232 3505 2232 Size: 1273 4911 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2796 4247 5631 5899 1632 Size: 1164 1451 1384 268 1917 Fragments arranged by size: 1917 1451 1384 1164 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 5083 6060 1203 Size: 3880 977 1327 Fragments arranged by size: 3880 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2818 1184 Size: 1634 4550 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3365 3397 3365 Size: 32 6152 AlwNI CAG_nnn'CTG Cuts at: 2465 3455 3927 5499 2465 Size: 990 472 1572 3150 Fragments arranged by size: 3150 1572 990 472 ApaLI G'TGCA_C Cuts at: 4241 5397 4241 Size: 1156 5028 ApoI r'AATT_y Cuts at: 1493 2246 3493 3719 3871 5980 1493 Size: 753 1247 226 152 2109 1697 Fragments arranged by size: 2109 1697 1247 753 226 152 AseI AT'TA_AT Cuts at: 160 1032 2337 3282 3405 4738 160 Size: 872 1305 945 123 1333 1606 Fragments arranged by size: 1606 1333 1305 945 872 123 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 3509 1870 Size: 73 1566 4545 Fragments arranged by size: 4545 1566 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2216 2249 3405 3438 2216 Size: 33 1156 33 4962 Fragments arranged by size: 4962 1156 33 33 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2232 3505 4960 618 Size: 325 217 627 445 1273 1455 1842 Fragments arranged by size: 1842 1455 1273 627 445 325 217 BanII G_rGCy'C Cuts at: 1391 2283 2512 2847 3503 1391 Size: 892 229 335 656 4072 Fragments arranged by size: 4072 892 656 335 229 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4375 4409 1903 Size: 34 2438 34 3678 Fragments arranged by size: 3678 2438 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3019 4076 5286 3019 Size: 1057 1210 3917 Fragments arranged by size: 3917 1210 1057 BclI T'GATC_A Cuts at: 1548 2629 1548 Size: 1081 5103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4795 136 Size: 107 122 71 631 605 3123 1525 Fragments arranged by size: 3123 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6179 6179 Size: 6184 BlpI GC'TnA_GC Cuts at: 2841 3601 2841 Size: 760 5424 Bme1580I G_kGCm'C Cuts at: 1790 4245 5401 1790 Size: 2455 1156 2573 Fragments arranged by size: 2573 2455 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2404 3915 4873 Size: 416 53 83 399 24 987 1511 958 Cuts at: 4873 442 Size: 1753 Fragments arranged by size: 1753 1511 987 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2496 2634 2733 4844 1239 Size: 1257 138 99 2111 2579 Fragments arranged by size: 2579 2111 1257 138 99 Bpu10I CC'TnA_GC Cuts at: 2766 2766 Size: 6184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4309 5174 5472 4309 Size: 865 298 5021 Fragments arranged by size: 5021 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4847 882 Size: 617 3348 2219 Fragments arranged by size: 3348 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3975 493 Size: 711 2771 2702 Fragments arranged by size: 2771 2702 711 BsaBI GATnn'nnATC Cuts at: 2628 6178 2628 Size: 3550 2634 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3991 4373 5008 275 Size: 53 83 186 936 2458 382 635 1451 Fragments arranged by size: 2458 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4617 5015 5289 5436 5847 4617 Size: 398 274 147 411 4954 Fragments arranged by size: 4954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5959 Fragments arranged by size: 5959 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6184 BseYI C'CCAG_C Cuts at: 3275 5387 5883 3275 Size: 2112 496 3576 Fragments arranged by size: 3576 2112 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5611 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2283 2512 2847 4245 4330 5401 2283 Size: 229 335 1398 85 1071 3066 Fragments arranged by size: 3066 1398 1071 335 229 85 BsmI GAATG_Cn' Cuts at: 3695 3788 3695 Size: 93 6091 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2512 2847 3503 4245 4330 5401 Size: 399 493 229 335 656 742 85 1071 Cuts at: 5401 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 656 493 399 335 229 85 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 4073 5803 4073 Size: 1730 4454 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3539 844 Size: 2695 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4071 1394 Size: 429 2248 3507 Fragments arranged by size: 3507 2248 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2154 3205 4679 4853 65 Size: 1183 906 1051 1474 174 1396 Fragments arranged by size: 1474 1396 1183 1051 906 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4828 5015 1700 Size: 110 3018 187 2869 Fragments arranged by size: 3018 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 4244 5256 4244 Size: 1012 5172 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6184 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstYI r'GATC_y Cuts at: 1972 3514 3881 4266 4283 5735 5821 5833 6179 Size: 1542 367 385 17 1452 86 12 346 Cuts at: 6179 1972 Size: 1977 Fragments arranged by size: 1977 1542 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4693 Fragments arranged by size: 4693 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5445 Fragments arranged by size: 5445 683 56 BtsI GCAGTG_nn' Cuts at: 3700 4494 4514 3700 Size: 794 20 5370 Fragments arranged by size: 5370 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 3067 3488 3067 Size: 421 5763 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 3631 4520 Size: 54 1425 50 275 18 264 1537 889 Cuts at: 4520 6149 8 Size: 1629 43 Fragments arranged by size: 1629 1537 1425 889 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4114 1353 Size: 2761 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4771 5145 5291 387 Size: 1064 138 3182 374 146 1280 Fragments arranged by size: 3182 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2281 2510 2845 2281 Size: 229 335 5620 Fragments arranged by size: 5620 335 229 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2496 2634 2733 2796 4247 4844 5631 Size: 393 864 138 99 63 1451 597 787 Cuts at: 5631 5899 1239 Size: 268 1524 Fragments arranged by size: 1524 1451 864 787 597 393 268 138 99 63 EcoO109I rG'GnC_Cy Cuts at: 3428 3428 Size: 6184 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2125 2821 2125 Size: 696 5488 FspI TGC'GCA Cuts at: 4690 4690 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 5331 1678 Size: 3653 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4825 4857 4899 4931 1373 Size: 32 135 32 3253 32 42 32 2626 Fragments arranged by size: 3253 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2254 3528 3774 669 Size: 988 281 316 1274 246 3079 Fragments arranged by size: 3079 1274 988 316 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 2236 3509 2236 Size: 1273 4911 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2014 5298 5482 1305 Size: 709 3284 184 2007 Fragments arranged by size: 3284 2007 709 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 10 Size: 54 1425 343 264 4098 Fragments arranged by size: 4098 1425 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 3407 4142 4501 4660 518 Size: 648 2241 735 359 159 2042 Fragments arranged by size: 2241 2042 735 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3132 3600 4277 5425 5670 1606 Size: 1526 468 677 1148 245 2120 Fragments arranged by size: 2120 1526 1148 677 468 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4693 Fragments arranged by size: 4693 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 3542 5087 1132 Size: 97 2313 1545 2229 Fragments arranged by size: 2313 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6184 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6184 PfoI T'CCnGG_A Cuts at: 2611 2611 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3428 3428 Size: 6184 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3057 3399 3754 3057 Size: 342 355 5487 Fragments arranged by size: 5487 355 342 PstI C_TGCA'G Cuts at: 830 3197 3268 3536 830 Size: 2367 71 268 3478 Fragments arranged by size: 3478 2367 268 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 3132 3132 Size: 6184 SacI G_AGCT'C Cuts at: 2283 2512 2847 2283 Size: 229 335 5620 Fragments arranged by size: 5620 335 229 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 2903 3193 3264 3532 4667 5348 5539 Size: 219 1858 290 71 268 1135 681 191 Cuts at: 5539 826 Size: 1471 Fragments arranged by size: 1858 1471 1135 681 290 268 219 191 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6184 SmaI CCC'GGG Cuts at: 1872 3511 1872 Size: 1639 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4288 5189 5451 820 Size: 197 926 2345 901 262 1553 Fragments arranged by size: 2345 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 4108 5 Size: 47 4056 2081 Fragments arranged by size: 4056 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6184 StyI C'CwwG_G Cuts at: 513 1065 2004 2672 3623 513 Size: 552 939 668 951 3074 Fragments arranged by size: 3074 951 939 668 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4211 4228 4381 4566 5011 325 Size: 1255 2631 17 153 185 445 1498 Fragments arranged by size: 2631 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2914 4430 6142 Size: 274 80 33 51 1443 937 1516 1712 Cuts at: 6142 96 Size: 138 Fragments arranged by size: 1712 1516 1443 937 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2076 2108 3208 3240 6144 6176 2076 Size: 32 1100 32 2904 32 2084 Fragments arranged by size: 2904 2084 1100 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6184 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2572 2572 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 3509 1870 Size: 1639 4545 XmnI GAAnn'nnTTC Cuts at: 2935 4313 2935 Size: 1378 4806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI