################################## # NO CUT ENZYMES ( 193 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I TseI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 112 GCGG CCGC(-3/-1) AhdI 263 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 373 AGCT AG^CT AlwNI 9 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 179 GGGCCC GGGCC^C AspEI 263 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 159 CCCGAG C^[CT]CG[GA]G BalI 316 TGGCCA TGG^CCA BanII 179 GGGCCC G[GA]GC[CT]^C BbsI 356 GAAGAC GAAGAC(2/6) BglII 131 AGATCT A^GATCT BmyI 179 GGGCCC G[AGT]GC[ACT]^C BpuAI 356 GAAGAC GAAGAC(2/6) BsaWI 168 TCCGGT [AT]^CCGG[AT] BseRI 162 GAGGAG GAGGAG(10/8) BsoBI 159 CCCGAG C^[CT]CG[GA]G Bsp1286I 179 GGGCCC G[AGT]GC[ACT]^C BspMI 153 ACCTGC ACCTGC(4/8) BstAPI 8 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 307 CCATTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 131 AGATCT [GA]^GATC[CT] Cac8I 4 GCTCGC GC[ACGT]^[ACGT]GC DdeI 380 CTGAG C^T[ACGT]AG DpnII 132 GATC ^GATC DraII 178 AGGGCCC [GA]G^G[ACGT]CC[CT] EaeI 316 TGGCCA [CT]^GGCC[GA] Eam1105I 263 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 73 GAAGAG CTCTTC(1/4) EcoO109I 178 AGGGCCC [GA]G^G[ACGT]CC[CT] HapII 169 CCGG C^CGG HgaI 115 GACGC GACGC(5/10) HpaII 169 CCGG C^CGG HphI 143 TCACC GGTGA(8/7) Ksp632I 73 GAAGAG CTCTTC(1/4) MboI 132 GATC ^GATC MflI 131 AGATCT [GA]^GATC[CT] MluNI 316 TGGCCA TGG^CCA MscI 316 TGGCCA TGG^CCA MslI 200 CACCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 169 CCGG C^CGG MwoI 8 GCAGACACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 132 GATC ^GATC NlaIV 179 GGGCCC GG[ACGT]^[ACGT]CC Sau3AI 132 GATC ^GATC Tsp45I 172 GTCAC ^GT[GC]AC TspRI 13 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 131 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BpmI 361 CTCCAG CTGGAG(16/14) 92 CTGGAG BsaJI 160 CCGAGG C^C[ACGT][ACGT]GG 202 CCCAGG BsmFI 209 GTCCC GGGAC(10/14) 271 GTCCC BsrI 26 ACTGG ACTGG(1/-1) 33 ACTGG BssKI 91 CCTGG ^CC[ACGT]GG 203 CCAGG BstNI 91 CCTGG CC^[AT]GG 203 CCAGG EcoRII 91 CCTGG ^CC[AT]GG 203 CCAGG MvaI 91 CCTGG CC^[AT]GG 203 CCAGG PleI 359 GACTC GAGTC(4/5) 165 GAGTC ScrFI 91 CCTGG CC^[ACGT]GG 203 CCAGG TfiI 97 GATTC G^A[AT]TC 239 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### Cfr13I HaeIII HinfI MaeIII MboII MnlI Sau96I