(Circular) (Six-base) MAPSORT of: pFN21AE6524.seq Check: 4128 from: 1 to: 5095 pFN21AE6524 5095 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2167 2167 Size: 5095 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2905 278 Size: 53 83 186 936 1369 2468 Fragments arranged by size: 2468 1369 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2438 1937 Size: 501 4594 Acc65I G'GTAC_C Cuts at: 2416 2416 Size: 5095 AclI AA'CG_TT Cuts at: 1563 3222 3595 1563 Size: 1659 373 3063 Fragments arranged by size: 3063 1659 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3158 4542 4810 1632 Size: 1526 1384 268 1917 Fragments arranged by size: 1917 1526 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4898 AflIII A'CryG_T Cuts at: 1203 3994 4971 1203 Size: 2791 977 1327 Fragments arranged by size: 2791 1327 977 AgeI A'CCGG_T Cuts at: 3926 3926 Size: 5095 AhdI GACnn_n'nnGTC Cuts at: 2273 3824 2273 Size: 1551 3544 AleI CACnn'nnGTG Cuts at: 1166 2209 1166 Size: 1043 4052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5095 AlwNI CAG_nnn'CTG Cuts at: 2019 2838 4410 2019 Size: 819 1572 2704 Fragments arranged by size: 2704 1572 819 ApaI G_GGCC'C Cuts at: 2188 2188 Size: 5095 ApaLI G'TGCA_C Cuts at: 3152 4308 3152 Size: 1156 3939 ApoI r'AATT_y Cuts at: 1493 2404 2630 2782 4891 1493 Size: 911 226 152 2109 1697 Fragments arranged by size: 2109 1697 911 226 152 AseI AT'TA_AT Cuts at: 160 1032 3649 160 Size: 872 2617 1606 Fragments arranged by size: 2617 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5095 AvaI C'yCGr_G Cuts at: 1870 1943 2164 2420 1870 Size: 73 221 256 4545 Fragments arranged by size: 4545 256 221 73 BamHI G'GATC_C Cuts at: 2425 2792 2425 Size: 367 4728 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2416 3871 618 Size: 325 217 627 629 1455 1842 Fragments arranged by size: 1842 1455 629 627 325 217 BanII G_rGCy'C Cuts at: 1391 2188 2414 1391 Size: 797 226 4072 Fragments arranged by size: 4072 797 226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2368 928 Size: 971 469 3655 Fragments arranged by size: 3655 971 469 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3286 3320 1903 Size: 34 1349 34 3678 Fragments arranged by size: 3678 1349 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2987 4197 2987 Size: 1210 3885 BclI T'GATC_A Cuts at: 1548 1548 Size: 5095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3706 136 Size: 107 122 71 631 605 2034 1525 Fragments arranged by size: 2034 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2136 5090 2136 Size: 2954 2141 BlpI GC'TnA_GC Cuts at: 2512 2512 Size: 5095 Bme1580I G_kGCm'C Cuts at: 1790 2188 3156 4312 1790 Size: 398 968 1156 2573 Fragments arranged by size: 2573 1156 968 398 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5095 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2826 3784 442 Size: 416 53 83 399 24 1409 958 1753 Fragments arranged by size: 1753 1409 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5095 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2118 2351 3755 1239 Size: 879 233 1404 2579 Fragments arranged by size: 2579 1404 879 233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3220 4085 4383 3220 Size: 865 298 3932 Fragments arranged by size: 3932 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3758 882 Size: 617 2259 2219 Fragments arranged by size: 2259 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2886 493 Size: 711 1682 2702 Fragments arranged by size: 2702 1682 711 BsaBI GATnn'nnATC Cuts at: 5089 5089 Size: 5095 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2902 3284 3919 275 Size: 53 83 186 936 1369 382 635 1451 Fragments arranged by size: 1451 1369 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2173 3528 3926 4200 4347 4758 2173 Size: 1355 398 274 147 411 2510 Fragments arranged by size: 2510 1355 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 1178 Size: 30 165 30 606 30 4234 Fragments arranged by size: 4234 606 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2182 1186 Size: 996 4099 BseYI C'CCAG_C Cuts at: 2187 4298 4794 2187 Size: 2111 496 2488 Fragments arranged by size: 2488 2111 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2545 3306 4334 1540 Size: 275 186 544 761 1028 2301 Fragments arranged by size: 2301 1028 761 544 275 186 BsiHKAI G_wGCw'C Cuts at: 3156 3241 4312 3156 Size: 85 1071 3939 Fragments arranged by size: 3939 1071 85 BsmI GAATG_Cn' Cuts at: 2606 2699 2606 Size: 93 5002 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2188 2414 3156 3241 4312 1391 Size: 399 398 226 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 398 226 85 BspEI T'CCGG_A Cuts at: 4758 4758 Size: 5095 BspHI T'CATG_A Cuts at: 2984 4714 2984 Size: 1730 3365 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2167 2450 844 Size: 1323 283 3489 Fragments arranged by size: 3489 1323 283 BsrBI CCG'CTC Cuts at: 1394 1823 2982 1394 Size: 429 1159 3507 Fragments arranged by size: 3507 1159 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3590 3764 65 Size: 1183 2342 174 1396 Fragments arranged by size: 2342 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3739 3926 1700 Size: 110 1929 187 2869 Fragments arranged by size: 2869 1929 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5095 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5095 BssSI C'ACGA_G Cuts at: 3155 4167 3155 Size: 1012 4083 BstAPI GCAn_nnn'nTGC Cuts at: 794 2019 794 Size: 1225 3870 BstBI TT'CG_AA Cuts at: 3899 3899 Size: 5095 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5095 BstXI CCAn_nnnn'nTGG Cuts at: 2319 2319 Size: 5095 BstYI r'GATC_y Cuts at: 1972 2136 2425 2792 3177 3194 4646 4732 4744 Size: 164 289 367 385 17 1452 86 12 Cuts at: 4744 5090 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 289 164 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3604 Fragments arranged by size: 3604 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4356 Fragments arranged by size: 4356 683 56 BtsI GCAGTG_nn' Cuts at: 2017 2611 3405 3425 2017 Size: 594 794 20 3687 Fragments arranged by size: 3687 794 594 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5095 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5060 DraI TTT'AAA Cuts at: 2399 2399 Size: 5095 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4102 809 Size: 507 2786 1802 Fragments arranged by size: 2786 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2321 2542 3431 Size: 54 1425 50 275 18 491 221 889 Cuts at: 3431 5060 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 491 275 221 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2542 1537 Size: 275 730 4090 Fragments arranged by size: 4090 730 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2073 3025 1353 Size: 720 952 3423 Fragments arranged by size: 3423 952 720 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3682 4056 4202 387 Size: 1064 138 2093 374 146 1280 Fragments arranged by size: 2093 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2118 2351 3158 3755 4542 4810 1239 Size: 393 486 233 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 597 486 393 268 233 EcoO109I rG'GnC_Cy Cuts at: 2184 2184 Size: 5095 EcoRI G'AATT_C Cuts at: 2404 2782 2404 Size: 378 4717 FspI TGC'GCA Cuts at: 3601 3601 Size: 5095 HaeII r_GCGC'y Cuts at: 1678 4242 1678 Size: 2564 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2259 2291 3736 3768 3810 Size: 32 135 32 687 32 1445 32 42 Cuts at: 3810 3842 1373 Size: 32 2626 Fragments arranged by size: 2626 1445 687 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2439 2685 669 Size: 988 281 501 246 3079 Fragments arranged by size: 3079 988 501 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5095 HpaI GTT'AAC Cuts at: 2685 2685 Size: 5095 KpnI G_GTAC'C Cuts at: 2420 2420 Size: 5095 MfeI C'AATT_G Cuts at: 2694 2694 Size: 5095 MluI A'CGCG_T Cuts at: 4971 4971 Size: 5095 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4209 4393 1305 Size: 2904 184 2007 Fragments arranged by size: 2904 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2323 10 Size: 54 1425 343 491 2782 Fragments arranged by size: 2782 1425 491 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2209 3053 3412 3571 518 Size: 648 1043 844 359 159 2042 Fragments arranged by size: 2042 1043 844 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2511 3188 4336 4581 1606 Size: 905 677 1148 245 2120 Fragments arranged by size: 2120 1148 905 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3604 Fragments arranged by size: 3604 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5095 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5095 NotI GC'GGCC_GC Cuts at: 2542 2542 Size: 5095 NspI r_CATG'y Cuts at: 1132 1229 2453 3998 1132 Size: 97 1224 1545 2229 Fragments arranged by size: 2229 1545 1224 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5095 PciI A'CATG_T Cuts at: 3994 3994 Size: 5095 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5095 PfoI T'CCnGG_A Cuts at: 2095 2095 Size: 5095 PmeI GTTT'AAAC Cuts at: 2399 2399 Size: 5095 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3164 3196 4677 4709 3164 Size: 32 1481 32 3550 Fragments arranged by size: 3550 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5095 PsiI TTA'TAA Cuts at: 2665 2665 Size: 5095 PspOMI G'GGCC_C Cuts at: 2184 2184 Size: 5095 PstI C_TGCA'G Cuts at: 830 2447 830 Size: 1617 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5095 SalI G'TCGA_C Cuts at: 1936 2437 1936 Size: 501 4594 SbfI CC_TGCA'GG Cuts at: 2447 2447 Size: 5095 ScaI AGT'ACT Cuts at: 3343 5055 3343 Size: 1712 3383 SfcI C'TryA_G Cuts at: 826 1045 2443 3578 4259 4450 826 Size: 219 1398 1135 681 191 1471 Fragments arranged by size: 1471 1398 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5095 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5095 SmaI CCC'GGG Cuts at: 1872 2422 1872 Size: 550 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3199 4100 4362 820 Size: 197 926 1256 901 262 1553 Fragments arranged by size: 1553 1256 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2886 493 Size: 2393 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5095 SphI G_CATG'C Cuts at: 1132 2453 1132 Size: 1321 3774 SspI AAT'ATT Cuts at: 5 52 3019 5 Size: 47 2967 2081 Fragments arranged by size: 2967 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5095 StyI C'CwwG_G Cuts at: 513 1065 2004 2534 513 Size: 552 939 530 3074 Fragments arranged by size: 3074 939 552 530 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2221 3122 3139 3292 3477 3922 325 Size: 1255 641 901 17 153 185 445 1498 Fragments arranged by size: 1498 1255 901 641 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3341 5053 96 Size: 274 80 33 51 1443 1364 1712 138 Fragments arranged by size: 1712 1443 1364 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2009 2041 5055 5087 2009 Size: 32 3014 32 2017 Fragments arranged by size: 3014 2017 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5095 XbaI T'CTAG_A Cuts at: 2431 2431 Size: 5095 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5095 XmaI C'CCGG_G Cuts at: 1870 2420 1870 Size: 550 4545 XmnI GAAnn'nnTTC Cuts at: 3224 3224 Size: 5095 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2903 276 Size: 53 83 186 936 1369 2468 Fragments arranged by size: 2468 1369 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI