Restriction map for
pFN21AE3036
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbvCI BcgI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HaeIII HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I MaeI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MwoI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 6 CGCG CG^CG
AflIII 174 ACGTGT A^C[GA][CT]GT
AocI 479 CCTCAGG CC^T[ACGT]AGG
AvaII 42 GGTCC G^G[AT]CC
BamHI 486 GGATCC G^GATCC
BbrPI 173 CACGTG CAC^GTG
BbsI 163 GAAGAC GAAGAC(2/6)
BciVI 116 GTATCC GTATCC(6/5)
BpuAI 163 GAAGAC GAAGAC(2/6)
BsaAI 173 CACGTG [CT]AC^GT[GA]
BseRI 12 GAGGAG GAGGAG(10/8)
BsgI 141 CTGCAC GTGCAG(16/14)
BsiYI 5 CCGCGGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 5 CCGCGGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 43 GTCCC GGGAC(10/14)
BspMI 275 GCAGGT ACCTGC(4/8)
BsrFI 34 GCCGGC [GA]^CCGG[CT]
BstF5I 17 GGATG GGATG(2/0)
BstUI 6 CGCG CG^CG
BstYI 486 GGATCC [GA]^GATC[CT]
Bsu36I 479 CCTCAGG CC^T[ACGT]AGG
Cac8I 34 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 49 GCGC GCG^C
Cfr10I 34 GCCGGC [GA]^CCGG[CT]
Cfr13I 42 GGTCC G^G[ACGT]CC
DpnII 487 GATC ^GATC
DraII 41 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 5 CCGCGG C^C[GA][CT]GG
Eco81I 479 CCTCAGG CC^T[ACGT]AGG
EcoO109I 41 GGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 7 GCGGG CCCGC(4/6)
FokI 17 GGATG GGATG(9/13)
HapII 35 CCGG C^CGG
HhaI 49 GCGC GCG^C
HinP1I 49 GCGC G^CGC
HpaII 35 CCGG C^CGG
KspI 5 CCGCGG CCGC^GG
MaeII 174 ACGT A^CGT
MaeIII 475 GTTAC ^GT[ACGT]AC
MboI 487 GATC ^GATC
MflI 486 GGATCC [GA]^GATC[CT]
MspA1I 5 CCGCGG C[AC]G^C[GT]G
MspI 35 CCGG C^CGG
MvnI 6 CGCG CG^CG
NaeI 34 GCCGGC GCC^GGC
NdeII 487 GATC ^GATC
NgoMIV 34 GCCGGC G^CCGGC
PmaCI 173 CACGTG CAC^GTG
PmlI 173 CACGTG CAC^GTG
PpuMI 41 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 256 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 5 CCGCGG CCGC^GG
Sau3AI 487 GATC ^GATC
Sau96I 42 GGTCC G^G[ACGT]CC
SmlI 182 CTCAAG C^T[CT][GA]AG
TaqI 438 TCGA T^CGA
TthHB8I 438 TCGA T^CGA
XhoII 486 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 95 GTAC GT^AC
429 GTAC
AluI 147 AGCT AG^CT
205 AGCT
AlwI 487 GATCC GGATC(4/5)
486 GGATC
BbvI 51 GCTGC GCAGC(8/12)
54 GCTGC
BsrI 295 ACTGG ACTGG(1/-1)
131 CCAGT
BssKI 63 CCTGG ^CC[ACGT]GG
121 CCAGG
BstNI 63 CCTGG CC^[AT]GG
121 CCAGG
DdeI 91 CTCAG C^T[ACGT]AG
480 CTCAG
EcoRII 63 CCTGG ^CC[AT]GG
121 CCAGG
MboII 163 GAAGA GAAGA(8/7)
75 TCTTC
MseI 322 TTAA T^TAA
340 TTAA
MvaI 63 CCTGG CC^[AT]GG
121 CCAGG
PleI 127 GAGTC GAGTC(4/5)
261 GAGTC
PstI 273 CTGCAG CTGCA^G
420 CTGCAG
RsaI 95 GTAC GT^AC
429 GTAC
ScrFI 63 CCTGG CC^[ACGT]GG
121 CCAGG
SfaNI 24 GATGC GCATC(5/9)
242 GCATC
TfiI 105 GATTC G^A[AT]TC
369 GATTC
Tru9I 322 TTAA T^TAA
340 TTAA
TseI 51 GCTGC G^C[AT]GC
54 GCTGC
TspRI 132 CAGTG CA[GC]TG[ACGT][ACGT]^
238 CAGTG
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AciI BsaJI Fnu4HI HinfI ItaI
MnlI NlaIII NlaIV SfcI