Restriction map for
pFN21AE3090
##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT14I
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII HphI KasI KpnI KspI
MaeI MaeII MamI MflI MluI
MroI MslI MspA1I MspI MvaI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 199 GAATTT [GA]^AATT[CT]
ApoI 199 GAATTT [GA]^AATT[CT]
Asp700I 145 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 317 GAGCTC G[AT]GC[AT]^C
AvaII 53 GGACC G^G[AT]CC
BanI 690 GGTGCC G^G[CT][GA]CC
BanII 317 GAGCTC G[GA]GC[CT]^C
BbsI 718 GAAGAC GAAGAC(2/6)
BlpI 319 GCTCAGC GC^T[ACGT]AGC
BmyI 317 GAGCTC G[AGT]GC[ACT]^C
BpmI 542 CTCCAG CTGGAG(16/14)
Bpu1102I 319 GCTCAGC GC^T[ACGT]AGC
BpuAI 718 GAAGAC GAAGAC(2/6)
BsiHKAI 317 GAGCTC G[AT]GC[AT]^C
BsmFI 52 GGGAC GGGAC(10/14)
Bsp1286I 317 GAGCTC G[AGT]GC[ACT]^C
BsrDI 176 GCAATG GCAATG(2/0)
CelII 319 GCTCAGC GC^T[ACGT]AGC
Cfr13I 53 GGACC G^G[ACGT]CC
DpnII 383 GATC ^GATC
DraI 724 TTTAAA TTT^AAA
DraII 52 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 586 GAAGAG CTCTTC(1/4)
EcoO109I 52 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 435 ATGCAT ATGCA^T
HincII 102 GTCAAC GT[CT]^[GA]AC
HindII 102 GTCAAC GT[CT]^[GA]AC
HindIII 619 AAGCTT A^AGCTT
Ksp632I 586 GAAGAG CTCTTC(1/4)
MaeIII 395 GTGAC ^GT[ACGT]AC
MboI 383 GATC ^GATC
MfeI 127 CAATTG C^AATTG
MunI 127 CAATTG C^AATTG
MwoI 319 GCTCAGCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 293 CATATG CA^TATG
NdeII 383 GATC ^GATC
NsiI 435 ATGCAT ATGCA^T
NspI 550 ACATGC [GA]CATG^[CT]
PpuMI 52 GGGACCC [GA]G^G[AT]CC[CT]
PstI 92 CTGCAG CTGCA^G
SacI 317 GAGCTC GAGCT^C
SapI 586 GAAGAGC GCTCTTC(1/4)
Sau3AI 383 GATC ^GATC
Sau96I 53 GGACC G^G[ACGT]CC
SfcI 92 CTGCAG C^T[GA][CT]AG
SmlI 191 CTCAAG C^T[CT][GA]AG
SspI 608 AATATT AAT^ATT
Tsp45I 395 GTGAC ^GT[GC]AC
XmnI 145 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 10 CCGC CCGC(-3/-1)
57 CCGC
AfaI 188 GTAC GT^AC
773 GTAC
BalI 2 TGGCCA TGG^CCA
498 TGGCCA
BbvI 23 GCTGC GCAGC(8/12)
91 GCTGC
BsiYI 74 CCATCGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
507 CCTTGAATTGG
BslI 74 CCATCGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
507 CCTTGAATTGG
BsmI 433 GAATGC GAATGC(1/-1)
596 GCATTC
EaeI 2 TGGCCA [CT]^GGCC[GA]
498 TGGCCA
FauI 9 CCCGC CCCGC(4/6)
56 CCCGC
Fnu4HI 23 GCTGC GC^[ACGT]GC
91 GCTGC
HaeIII 3 GGCC GG^CC
499 GGCC
HinfI 427 GAATC G^A[ACGT]TC
521 GAATC
ItaI 23 GCTGC GC^[ACGT]GC
91 GCTGC
MluNI 2 TGGCCA TGG^CCA
498 TGGCCA
MscI 2 TGGCCA TGG^CCA
498 TGGCCA
RsaI 188 GTAC GT^AC
773 GTAC
ScaI 187 AGTACT AGT^ACT
772 AGTACT
TfiI 427 GAATC G^A[AT]TC
521 GAATC
TseI 23 GCTGC G^C[AT]GC
91 GCTGC
TspRI 659 CACTG CA[GC]TG[ACGT][ACGT]^
704 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BsmAI BsrI BstF5I Cac8I
DdeI FokI MboII MnlI MseI
NlaIII NlaIV SfaNI TaqI Tru9I
Tsp509I TthHB8I