(Circular) (Six-base) MAPSORT of: pFN21AE3090.seq Check: 7968 from: 1 to: 5485 pFN21AE3090 5485 bp With 182 enzymes: SgfI * March 28, 2012 12:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3295 278 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2828 1937 Size: 891 4594 Acc65I G'GTAC_C Cuts at: 2806 2806 Size: 5485 AclI AA'CG_TT Cuts at: 1563 3612 3985 1563 Size: 2049 373 3063 Fragments arranged by size: 3063 2049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 3548 4932 5200 1632 Size: 602 1314 1384 268 1917 Fragments arranged by size: 1917 1384 1314 602 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5288 AflIII A'CryG_T Cuts at: 1203 4384 5361 1203 Size: 3181 977 1327 Fragments arranged by size: 3181 1327 977 AgeI A'CCGG_T Cuts at: 4316 4316 Size: 5485 AhdI GACnn_n'nnGTC Cuts at: 4214 4214 Size: 5485 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5485 AlwNI CAG_nnn'CTG Cuts at: 3228 4800 3228 Size: 1572 3913 ApaLI G'TGCA_C Cuts at: 3542 4698 3542 Size: 1156 4329 ApoI r'AATT_y Cuts at: 1493 2204 2794 3020 3172 5281 1493 Size: 711 590 226 152 2109 1697 Fragments arranged by size: 2109 1697 711 590 226 152 AseI AT'TA_AT Cuts at: 160 1032 4039 160 Size: 872 3007 1606 Fragments arranged by size: 3007 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 AvaI C'yCGr_G Cuts at: 1870 1943 2810 1870 Size: 73 867 4545 Fragments arranged by size: 4545 867 73 BamHI G'GATC_C Cuts at: 2815 3182 2815 Size: 367 5118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2695 2806 4261 618 Size: 325 217 627 908 111 1455 1842 Fragments arranged by size: 1842 1455 908 627 325 217 111 BanII G_rGCy'C Cuts at: 1391 2326 2804 1391 Size: 935 478 4072 Fragments arranged by size: 4072 935 478 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2730 928 Size: 971 831 3683 Fragments arranged by size: 3683 971 831 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3676 3710 1903 Size: 34 1739 34 3678 Fragments arranged by size: 3678 1739 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3377 4587 3377 Size: 1210 4275 BclI T'GATC_A Cuts at: 1548 1548 Size: 5485 BfrBI ATG'CAT Cuts at: 2442 2442 Size: 5485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4096 136 Size: 107 122 71 631 605 2424 1525 Fragments arranged by size: 2424 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5480 5480 Size: 5485 BlpI GC'TnA_GC Cuts at: 2325 2902 2325 Size: 577 4908 Bme1580I G_kGCm'C Cuts at: 1790 3546 4702 1790 Size: 1756 1156 2573 Fragments arranged by size: 2573 1756 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5485 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3216 4174 442 Size: 416 53 83 399 24 1799 958 1753 Fragments arranged by size: 1799 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2532 4145 1239 Size: 1293 1613 2579 Fragments arranged by size: 2579 1613 1293 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2181 3610 4475 4773 2181 Size: 1429 865 298 2893 Fragments arranged by size: 2893 1429 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4148 882 Size: 617 2649 2219 Fragments arranged by size: 2649 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3276 493 Size: 711 2072 2702 Fragments arranged by size: 2702 2072 711 BsaBI GATnn'nnATC Cuts at: 5479 5479 Size: 5485 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3292 3674 4309 275 Size: 53 83 186 936 1759 382 635 1451 Fragments arranged by size: 1759 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3918 4316 4590 4737 5148 3918 Size: 398 274 147 411 4255 Fragments arranged by size: 4255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5260 Fragments arranged by size: 5260 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5485 BseYI C'CCAG_C Cuts at: 4688 5184 4688 Size: 496 4989 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4912 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2935 3696 4724 1540 Size: 275 186 934 761 1028 2301 Fragments arranged by size: 2301 1028 934 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2326 3546 3631 4702 2326 Size: 1220 85 1071 3109 Fragments arranged by size: 3109 1220 1071 85 BsmI GAATG_Cn' Cuts at: 2444 2601 2996 3089 2444 Size: 157 395 93 4840 Fragments arranged by size: 4840 395 157 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2326 2804 3546 3631 4702 1391 Size: 399 536 478 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 536 478 399 85 BspEI T'CCGG_A Cuts at: 5148 5148 Size: 5485 BspHI T'CATG_A Cuts at: 3374 5104 3374 Size: 1730 3755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2840 844 Size: 1996 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3372 1394 Size: 429 1549 3507 Fragments arranged by size: 3507 1549 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2188 3980 4154 65 Size: 1183 940 1792 174 1396 Fragments arranged by size: 1792 1396 1183 940 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4129 4316 1700 Size: 110 2319 187 2869 Fragments arranged by size: 2869 2319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5485 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5485 BssSI C'ACGA_G Cuts at: 3545 4557 3545 Size: 1012 4473 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5485 BstBI TT'CG_AA Cuts at: 4289 4289 Size: 5485 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5485 BstYI r'GATC_y Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480 Size: 843 367 385 17 1452 86 12 346 Cuts at: 5480 1972 Size: 1977 Fragments arranged by size: 1977 1452 843 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3994 Fragments arranged by size: 3994 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2077 487 Size: 683 56 851 3895 Fragments arranged by size: 3895 851 683 56 BtsI GCAGTG_nn' Cuts at: 3001 3795 3815 3001 Size: 794 20 4671 Fragments arranged by size: 4671 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5450 DraI TTT'AAA Cuts at: 2731 2789 2731 Size: 58 5427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4492 809 Size: 507 3176 1802 Fragments arranged by size: 3176 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2503 2932 Size: 54 1425 50 275 18 177 496 429 Cuts at: 2932 3821 5450 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 496 429 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2932 1537 Size: 275 1120 4090 Fragments arranged by size: 4090 1120 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2586 3415 1353 Size: 1233 829 3423 Fragments arranged by size: 3423 1233 829 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4072 4446 4592 387 Size: 1064 138 2483 374 146 1280 Fragments arranged by size: 2483 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2324 2324 Size: 5485 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2532 3548 4145 4932 5200 1239 Size: 393 602 298 1016 597 787 268 1524 Fragments arranged by size: 1524 1016 787 602 597 393 298 268 EcoO109I rG'GnC_Cy Cuts at: 2058 2058 Size: 5485 EcoRI G'AATT_C Cuts at: 2794 3172 2794 Size: 378 5107 FspI TGC'GCA Cuts at: 3991 3991 Size: 5485 HaeII r_GCGC'y Cuts at: 1678 4632 1678 Size: 2954 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2452 2484 4126 4158 4200 Size: 32 135 32 880 32 1642 32 42 Cuts at: 4200 4232 1373 Size: 32 2626 Fragments arranged by size: 2626 1642 880 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2109 2829 3075 669 Size: 988 281 171 720 246 3079 Fragments arranged by size: 3079 988 720 281 246 171 HindIII A'AGCT_T Cuts at: 748 2624 748 Size: 1876 3609 HpaI GTT'AAC Cuts at: 3075 3075 Size: 5485 KpnI G_GTAC'C Cuts at: 2810 2810 Size: 5485 MfeI C'AATT_G Cuts at: 2132 3084 2132 Size: 952 4533 MluI A'CGCG_T Cuts at: 5361 5361 Size: 5485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4599 4783 1305 Size: 3294 184 2007 Fragments arranged by size: 3294 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2505 10 Size: 54 1425 343 177 496 2990 Fragments arranged by size: 2990 1425 496 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3443 3802 3961 518 Size: 648 2277 359 159 2042 Fragments arranged by size: 2277 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2901 3578 4726 4971 1606 Size: 1295 677 1148 245 2120 Fragments arranged by size: 2120 1295 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3994 Fragments arranged by size: 3994 939 552 NdeI CA'TA_TG Cuts at: 387 2299 387 Size: 1912 3573 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5485 NotI GC'GGCC_GC Cuts at: 2932 2932 Size: 5485 NsiI A_TGCA'T Cuts at: 2444 2444 Size: 5485 NspI r_CATG'y Cuts at: 1132 1229 2559 2843 4388 1132 Size: 97 1330 284 1545 2229 Fragments arranged by size: 2229 1545 1330 284 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5485 PciI A'CATG_T Cuts at: 4384 4384 Size: 5485 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5485 PmeI GTTT'AAAC Cuts at: 2789 2789 Size: 5485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3554 3586 5067 5099 3554 Size: 32 1481 32 3940 Fragments arranged by size: 3940 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2058 2058 Size: 5485 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5485 PsiI TTA'TAA Cuts at: 2634 3055 2634 Size: 421 5064 PstI C_TGCA'G Cuts at: 830 2101 2837 830 Size: 1271 736 3478 Fragments arranged by size: 3478 1271 736 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5485 SacI G_AGCT'C Cuts at: 2326 2326 Size: 5485 SalI G'TCGA_C Cuts at: 1936 2827 1936 Size: 891 4594 SanDI GG'GwC_CC Cuts at: 2058 2058 Size: 5485 SapI GCTCTTCn'nnn_ Cuts at: 2586 2586 Size: 5485 SbfI CC_TGCA'GG Cuts at: 2837 2837 Size: 5485 ScaI AGT'ACT Cuts at: 2194 2779 3733 5445 2194 Size: 585 954 1712 2234 Fragments arranged by size: 2234 1712 954 585 SfcI C'TryA_G Cuts at: 826 1045 2097 2833 3968 4649 4840 826 Size: 219 1052 736 1135 681 191 1471 Fragments arranged by size: 1471 1135 1052 736 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5485 SmaI CCC'GGG Cuts at: 1872 2812 1872 Size: 940 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2196 3589 4490 4752 820 Size: 197 926 253 1393 901 262 1553 Fragments arranged by size: 1553 1393 926 901 262 253 197 SnaBI TAC'GTA Cuts at: 493 3276 493 Size: 2783 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5485 SphI G_CATG'C Cuts at: 1132 2843 1132 Size: 1711 3774 SspI AAT'ATT Cuts at: 5 52 2615 3409 5 Size: 47 2563 794 2081 Fragments arranged by size: 2563 2081 794 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5485 StyI C'CwwG_G Cuts at: 513 1065 2004 2924 513 Size: 552 939 920 3074 Fragments arranged by size: 3074 939 920 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3512 3529 3682 3867 4312 325 Size: 1255 1932 17 153 185 445 1498 Fragments arranged by size: 1932 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2192 2777 3731 Size: 274 80 33 51 1443 215 585 954 Cuts at: 3731 5443 96 Size: 1712 138 Fragments arranged by size: 1712 1443 954 585 274 215 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5445 5477 5445 Size: 32 5453 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5485 XbaI T'CTAG_A Cuts at: 2821 2821 Size: 5485 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5485 XmaI C'CCGG_G Cuts at: 1870 2810 1870 Size: 940 4545 XmnI GAAnn'nnTTC Cuts at: 2154 3614 2154 Size: 1460 4025 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3293 276 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI