################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MflI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 913 GAATTT [GA]^AATT[CT] AfaI 726 GTAC GT^AC Alw44I 965 GTGCAC G^TGCAC AocI 1069 CCTAAGG CC^T[ACGT]AGG ApaLI 965 GTGCAC G^TGCAC ApoI 913 GAATTT [GA]^AATT[CT] Asp700I 424 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1303 CCCGAG C^[CT]CG[GA]G BanI 501 GGCACC G^G[CT][GA]CC BbsI 808 GAAGAC GAAGAC(2/6) BbvCI 91 CCTCAGC CCTCAGC(-5/-2) BciVI 1242 GGATAC GTATCC(6/5) BpmI 379 CTCCAG CTGGAG(16/14) BpuAI 808 GAAGAC GAAGAC(2/6) BsaAI 727 TACGTG [CT]AC^GT[GA] BsaBI 42 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 232 GGTCTC GGTCTC(1/5) BseRI 235 CTCCTC GAGGAG(10/8) BsoBI 1303 CCCGAG C^[CT]CG[GA]G BspHI 776 TCATGA T^CATGA BstXI 1208 CCACATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1069 CCTAAGG CC^T[ACGT]AGG EarI 254 CTCTTC CTCTTC(1/4) Eco81I 1069 CCTAAGG CC^T[ACGT]AGG EcoT22I 1312 ATGCAT ATGCA^T FauI 800 GCGGG CCCGC(4/6) Ksp632I 254 CTCTTC CTCTTC(1/4) MaeII 728 ACGT A^CGT MamI 42 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 748 CAATTG C^AATTG MslI 1209 CACATCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1142 CAGCTG C[AC]G^C[GT]G MunI 748 CAATTG C^AATTG NsiI 1312 ATGCAT ATGCA^T PflMI 838 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 157 CTGCAG CTGCA^G PvuII 1142 CAGCTG CAG^CTG RcaI 776 TCATGA T^CATGA RsaI 726 GTAC GT^AC SacI 529 GAGCTC GAGCT^C SfcI 157 CTGCAG C^T[GA][CT]AG SmlI 130 CTTGAG C^T[CT][GA]AG SspI 299 AATATT AAT^ATT TfiI 15 GATTC G^A[AT]TC Van91I 838 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 424 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 800 GCGG CCGC(-3/-1) 896 GCGG AspHI 529 GAGCTC G[AT]GC[AT]^C 965 GTGCAC BsiHKAI 529 GAGCTC G[AT]GC[AT]^C 965 GTGCAC BsmFI 450 GGGAC GGGAC(10/14) 1119 GGGAC BsmI 753 GAATGC GAATGC(1/-1) 872 GAATGC BspMI 1038 ACCTGC ACCTGC(4/8) 495 GCAGGT BsrI 381 CCAGT ACTGG(1/-1) 925 CCAGT Cac8I 796 GCAGGC GC[ACGT]^[ACGT]GC 1088 GCCTGC HgaI 1318 GACGC GACGC(5/10) 1130 GCGTC MaeIII 899 GTGAC ^GT[ACGT]AC 1132 GTCAC MseI 166 TTAA T^TAA 212 TTAA NcoI 259 CCATGG C^CATGG 1238 CCATGG PleI 919 GACTC GAGTC(4/5) 1121 GACTC PpuMI 35 AGGACCT [GA]G^G[AT]CC[CT] 1186 AGGACCT Tru9I 166 TTAA T^TAA 212 TTAA Tsp45I 899 GTGAC ^GT[GC]AC 1132 GTCAC XbaI 187 TCTAGA T^CTAGA 1005 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AluI AlwI AlwNI AvaII BanII BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp509I TspRI