(Circular) (Six-base) MAPSORT of: pFN21AE2417.seq Check: 7264 from: 1 to: 6046 pFN21AE2417 6046 bp With 182 enzymes: SgfI * July 23, 2012 18:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2491 2491 Size: 6046 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3389 1937 Size: 1452 4594 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 3093 4109 5493 5761 1632 Size: 788 673 1016 1384 268 1917 Fragments arranged by size: 1917 1384 1016 788 673 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 4945 5922 1203 Size: 3742 977 1327 Fragments arranged by size: 3742 1327 977 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 3218 1166 Size: 2052 3994 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6046 AlwNI CAG_nnn'CTG Cuts at: 2936 3149 3152 3789 5361 2936 Size: 213 3 637 1572 3621 Fragments arranged by size: 3621 1572 637 213 3 ApaLI G'TGCA_C Cuts at: 2970 4103 5259 2970 Size: 1133 1156 3757 Fragments arranged by size: 3757 1156 1133 ApoI r'AATT_y Cuts at: 1493 2918 3355 3581 3733 5842 1493 Size: 1425 437 226 152 2109 1697 Fragments arranged by size: 2109 1697 1425 437 226 152 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 3308 3371 1870 Size: 73 1365 63 4545 Fragments arranged by size: 4545 1365 73 63 BamHI G'GATC_C Cuts at: 3376 3743 3376 Size: 367 5679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2506 3367 4822 618 Size: 325 217 627 719 861 1455 1842 Fragments arranged by size: 1842 1455 861 719 627 325 217 BanII G_rGCy'C Cuts at: 1391 2538 3063 3107 3309 3345 3365 1391 Size: 1147 525 44 202 36 20 4072 Fragments arranged by size: 4072 1147 525 202 44 36 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2820 928 Size: 971 921 4154 Fragments arranged by size: 4154 971 921 BbvCI CC'TCA_GC Cuts at: 2097 2097 Size: 6046 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3241 3938 5148 3241 Size: 697 1210 4139 Fragments arranged by size: 4139 1210 697 BclI T'GATC_A Cuts at: 1548 1548 Size: 6046 BfrBI ATG'CAT Cuts at: 3319 3319 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4657 136 Size: 107 122 71 631 605 2985 1525 Fragments arranged by size: 2985 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6041 6041 Size: 6046 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 2974 4107 5263 1790 Size: 1184 1133 1156 2573 Fragments arranged by size: 2573 1184 1156 1133 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2924 3777 4735 Size: 416 53 83 399 24 1507 853 958 Cuts at: 4735 442 Size: 1753 Fragments arranged by size: 1753 1507 958 853 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 4706 1239 Size: 1130 2337 2579 Fragments arranged by size: 2579 2337 1130 Bpu10I CC'TnA_GC Cuts at: 2097 2532 2097 Size: 435 5611 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2156 4171 5036 5334 2156 Size: 2015 865 298 2868 Fragments arranged by size: 2868 2015 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2243 4709 882 Size: 617 744 2466 2219 Fragments arranged by size: 2466 2219 744 617 BsaAI yAC'GTr Cuts at: 493 1204 2734 3837 493 Size: 711 1530 1103 2702 Fragments arranged by size: 2702 1530 1103 711 BsaBI GATnn'nnATC Cuts at: 2051 6040 2051 Size: 3989 2057 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3853 4235 4870 275 Size: 53 83 186 936 2320 382 635 1451 Fragments arranged by size: 2320 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4479 4877 5151 5298 5709 4479 Size: 398 274 147 411 4816 Fragments arranged by size: 4816 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2367 2397 1178 Size: 30 165 30 964 30 4827 Fragments arranged by size: 4827 964 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 1186 Size: 1045 5001 BseYI C'CCAG_C Cuts at: 2890 3019 3131 3142 5249 5745 2890 Size: 129 112 11 2107 496 3191 Fragments arranged by size: 3191 2107 496 129 112 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5473 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3496 4257 5285 1540 Size: 275 186 1495 761 1028 2301 Fragments arranged by size: 2301 1495 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2538 2974 4107 4192 5263 2538 Size: 436 1133 85 1071 3321 Fragments arranged by size: 3321 1133 1071 436 85 BsmI GAATG_Cn' Cuts at: 2764 2883 3557 3650 2764 Size: 119 674 93 5160 Fragments arranged by size: 5160 674 119 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2538 2974 3063 3107 3309 3345 3365 Size: 399 748 436 89 44 202 36 20 Cuts at: 3365 4107 4192 5263 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 748 742 436 399 202 89 85 44 36 20 BspEI T'CCGG_A Cuts at: 5709 5709 Size: 6046 BspHI T'CATG_A Cuts at: 2781 3935 5665 2781 Size: 1154 1730 3162 Fragments arranged by size: 3162 1730 1154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2491 3052 3401 844 Size: 1647 561 349 3489 Fragments arranged by size: 3489 1647 561 349 BsrBI CCG'CTC Cuts at: 1394 1823 3933 1394 Size: 429 2110 3507 Fragments arranged by size: 3507 2110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4541 4715 65 Size: 1183 3293 174 1396 Fragments arranged by size: 3293 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4690 4877 1700 Size: 110 2880 187 2869 Fragments arranged by size: 2880 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6046 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 4106 5118 4106 Size: 1012 5034 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6046 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6046 BstXI CCAn_nnnn'nTGG Cuts at: 3220 3220 Size: 6046 BstYI r'GATC_y Cuts at: 1972 3376 3743 4128 4145 5597 5683 5695 6041 Size: 1404 367 385 17 1452 86 12 346 Cuts at: 6041 1972 Size: 1977 Fragments arranged by size: 1977 1452 1404 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3075 3075 Size: 6046 BtgI C'CryG_G Cuts at: 513 1065 2264 3219 3243 513 Size: 552 1199 955 24 3316 Fragments arranged by size: 3316 1199 955 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5307 Fragments arranged by size: 5307 683 56 BtsI GCAGTG_nn' Cuts at: 2088 3562 4356 4376 2088 Size: 1474 794 20 3758 Fragments arranged by size: 3758 1474 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3493 4382 6011 Size: 54 1425 50 275 18 1663 889 1629 Cuts at: 6011 8 Size: 43 Fragments arranged by size: 1663 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3493 1537 Size: 275 1681 4090 Fragments arranged by size: 4090 1681 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2265 3976 1353 Size: 912 1711 3423 Fragments arranged by size: 3423 1711 912 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4633 5007 5153 387 Size: 1064 138 3044 374 146 1280 Fragments arranged by size: 3044 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2536 2536 Size: 6046 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2420 3093 4109 4706 5493 5761 Size: 393 737 51 673 1016 597 787 268 Cuts at: 5761 1239 Size: 1524 Fragments arranged by size: 1524 1016 787 737 673 597 393 268 51 EcoO109I rG'GnC_Cy Cuts at: 2041 2948 3192 2041 Size: 907 244 4895 Fragments arranged by size: 4895 907 244 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2450 3123 2450 Size: 673 5373 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 5193 1678 Size: 3515 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2221 2253 2763 2795 4687 Size: 32 135 32 649 32 510 32 1892 Cuts at: 4687 4719 4761 4793 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1892 649 510 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3390 3636 669 Size: 988 281 1452 246 3079 Fragments arranged by size: 3079 1452 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 2753 3645 2753 Size: 892 5154 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5160 5344 1305 Size: 3855 184 2007 Fragments arranged by size: 3855 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4224 Fragments arranged by size: 4224 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3218 4004 4363 4522 518 Size: 648 2052 786 359 159 2042 Fragments arranged by size: 2052 2042 786 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3149 3462 4139 5287 5532 1606 Size: 1543 313 677 1148 245 2120 Fragments arranged by size: 2120 1543 1148 677 313 245 NcoI C'CATG_G Cuts at: 513 1065 2264 3243 513 Size: 552 1199 979 3316 Fragments arranged by size: 3316 1199 979 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NsiI A_TGCA'T Cuts at: 3321 3321 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 3404 4949 1132 Size: 97 2175 1545 2229 Fragments arranged by size: 2229 2175 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6046 PciI A'CATG_T Cuts at: 4945 4945 Size: 6046 PflMI CCAn_nnn'nTGG Cuts at: 1108 2849 1108 Size: 1741 4305 PfoI T'CCnGG_A Cuts at: 2064 2719 2842 3228 2064 Size: 655 123 386 4882 Fragments arranged by size: 4882 655 386 123 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2041 3192 2041 Size: 1151 4895 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PstI C_TGCA'G Cuts at: 830 2166 3398 830 Size: 1336 1232 3478 Fragments arranged by size: 3478 1336 1232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 PvuII CAG'CTG Cuts at: 3149 3149 Size: 6046 SacI G_AGCT'C Cuts at: 2538 2538 Size: 6046 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SfcI C'TryA_G Cuts at: 826 1045 2162 3394 4529 5210 5401 826 Size: 219 1117 1232 1135 681 191 1471 Fragments arranged by size: 1471 1232 1135 1117 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 3373 1872 Size: 1501 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2135 4150 5051 5313 820 Size: 197 926 192 2015 901 262 1553 Fragments arranged by size: 2015 1553 926 901 262 197 192 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 2306 3970 5 Size: 47 2254 1664 2081 Fragments arranged by size: 2254 2081 1664 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6046 StyI C'CwwG_G Cuts at: 513 1065 2264 3098 3243 3485 513 Size: 552 1199 834 145 242 3074 Fragments arranged by size: 3074 1199 834 552 242 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4073 4090 4243 4428 4873 325 Size: 1255 2493 17 153 185 445 1498 Fragments arranged by size: 2493 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4292 6004 96 Size: 274 80 33 51 1443 2315 1712 138 Fragments arranged by size: 2315 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6006 6038 6006 Size: 32 6014 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6046 XbaI T'CTAG_A Cuts at: 2192 3010 3382 2192 Size: 818 372 4856 Fragments arranged by size: 4856 818 372 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 3371 1870 Size: 1501 4545 XmnI GAAnn'nnTTC Cuts at: 2433 4175 2433 Size: 1742 4304 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI