################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AluI 650 AGCT AG^CT Asp700I 331 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 454 GGATCC G^GATCC BbrPI 554 CACGTG CAC^GTG BbvI 840 GCAGC GCAGC(8/12) BfaI 302 CTAG C^TAG BsaAI 554 CACGTG [CT]AC^GT[GA] BseRI 646 GAGGAG GAGGAG(10/8) BsmAI 782 GAGAC GTCTC(1/5) BspLU11I 519 ACATGT A^CATGT BsrDI 123 CATTGC GCAATG(2/0) BsrI 785 ACTGG ACTGG(1/-1) BstBI 869 TTCGAA TT^CGAA BstYI 454 GGATCC [GA]^GATC[CT] DdeI 875 CTGAG C^T[ACGT]AG DsaI 42 CCATGG C^C[GA][CT]GG EcoNI 582 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 592 GATATC GAT^ATC EcoT14I 42 CCATGG C^C[AT][AT]GG Fnu4HI 840 GCAGC GC^[ACGT]GC HapII 384 CCGG C^CGG HpaII 384 CCGG C^CGG ItaI 840 GCAGC GC^[ACGT]GC MaeI 302 CTAG C^TAG MaeII 555 ACGT A^CGT MflI 454 GGATCC [GA]^GATC[CT] MseI 224 TTAA T^TAA MspI 384 CCGG C^CGG MwoI 94 GCGTTCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 42 CCATGG C^CATGG NspI 519 ACATGT [GA]CATG^[CT] NspV 869 TTCGAA TT^CGAA PflMI 445 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 554 CACGTG CAC^GTG PmlI 554 CACGTG CAC^GTG SfuI 869 TTCGAA TT^CGAA SspI 74 AATATT AAT^ATT StyI 42 CCATGG C^C[AT][AT]GG Tru9I 224 TTAA T^TAA TseI 840 GCAGC G^C[AT]GC Van91I 445 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 454 GGATCC [GA]^GATC[CT] XmnI 331 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 146 GTAC GT^AC 559 GTAC AflIII 519 ACATGT A^C[GA][CT]GT 555 ACGTGT AlwI 455 GATCC GGATC(4/5) 454 GGATC BbsI 312 GAAGAC GAAGAC(2/6) 577 GAAGAC BpuAI 312 GAAGAC GAAGAC(2/6) 577 GAAGAC BsaJI 42 CCATGG C^C[ACGT][ACGT]GG 152 CCTGGG BsmI 244 GAATGC GAATGC(1/-1) 387 GAATGC BspHI 263 TCATGA T^CATGA 761 TCATGA EcoRI 3 GAATTC G^AATTC 294 GAATTC HphI 190 GGTGA GGTGA(8/7) 606 GGTGA PleI 315 GACTC GAGTC(4/5) 549 GAGTC RcaI 263 TCATGA T^CATGA 761 TCATGA RsaI 146 GTAC GT^AC 559 GTAC TaqI 228 TCGA T^CGA 870 TCGA TfiI 48 GATTC G^A[AT]TC 853 GAATC TspRI 722 CACTG CA[GC]TG[ACGT][ACGT]^ 860 CACTG TthHB8I 228 TCGA T^CGA 870 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AcsI ApoI BsiYI BslI BssKI BstF5I BstNI DpnII EcoRII FokI HinfI MboI MboII MnlI MvaI NdeII NlaIII NlaIV Sau3AI ScrFI Tsp509I