(Circular) (Six-base) MAPSORT of: pFN21AE6450.seq Check: 9662 from: 1 to: 6286 pFN21AE6450 6286 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3629 1937 Size: 1692 4594 Acc65I G'GTAC_C Cuts at: 2560 3607 2560 Size: 1047 5239 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2018 4349 5733 6001 1632 Size: 386 2331 1384 268 1917 Fragments arranged by size: 2331 1917 1384 386 268 AfeI AGC'GCT Cuts at: 3280 3280 Size: 6286 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 2667 5185 6162 1203 Size: 1464 2518 977 1327 Fragments arranged by size: 2518 1464 1327 977 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 2614 5015 2614 Size: 2401 3885 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6286 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6286 AlwNI CAG_nnn'CTG Cuts at: 4029 5601 4029 Size: 1572 4714 ApaLI G'TGCA_C Cuts at: 2175 4343 5499 2175 Size: 2168 1156 2962 Fragments arranged by size: 2962 2168 1156 ApoI r'AATT_y Cuts at: 1493 2496 2638 2760 2792 2879 3339 3551 3595 Size: 1003 142 122 32 87 460 212 44 Cuts at: 3595 3821 3973 6082 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1003 460 226 212 152 142 122 87 44 32 AseI AT'TA_AT Cuts at: 160 1032 3297 4840 160 Size: 872 2265 1543 1606 Fragments arranged by size: 2265 1606 1543 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 3611 1870 Size: 73 1668 4545 Fragments arranged by size: 4545 1668 73 BamHI G'GATC_C Cuts at: 3616 3983 3616 Size: 367 5919 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2230 2560 3607 5062 618 Size: 325 217 627 443 330 1047 1455 1842 Fragments arranged by size: 1842 1455 1047 627 443 330 325 217 BanII G_rGCy'C Cuts at: 1391 2133 2894 3605 1391 Size: 742 761 711 4072 Fragments arranged by size: 4072 761 742 711 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2728 3373 928 Size: 971 829 645 3841 Fragments arranged by size: 3841 971 829 645 BbvCI CC'TCA_GC Cuts at: 2974 2974 Size: 6286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4477 4511 1903 Size: 34 2540 34 3678 Fragments arranged by size: 3678 2540 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3060 4178 5388 3060 Size: 1118 1210 3958 Fragments arranged by size: 3958 1210 1118 BclI T'GATC_A Cuts at: 1548 3190 3349 1548 Size: 1642 159 4485 Fragments arranged by size: 4485 1642 159 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4897 136 Size: 107 122 71 631 605 3225 1525 Fragments arranged by size: 3225 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2376 6281 2376 Size: 3905 2381 BlpI GC'TnA_GC Cuts at: 3275 3703 3275 Size: 428 5858 Bme1580I G_kGCm'C Cuts at: 1790 2179 2235 4347 5503 1790 Size: 389 56 2112 1156 2573 Fragments arranged by size: 2573 2112 1156 389 56 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4017 4975 442 Size: 416 53 83 399 24 2600 958 1753 Fragments arranged by size: 2600 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2541 2541 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2511 4946 1239 Size: 1272 2435 2579 Fragments arranged by size: 2579 2435 1272 Bpu10I CC'TnA_GC Cuts at: 2893 2974 2893 Size: 81 6205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2536 4411 5276 5574 2536 Size: 1875 865 298 3248 Fragments arranged by size: 3248 1875 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4949 882 Size: 617 3450 2219 Fragments arranged by size: 3450 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4077 493 Size: 711 2873 2702 Fragments arranged by size: 2873 2702 711 BsaBI GATnn'nnATC Cuts at: 6280 6280 Size: 6286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4093 4475 5110 275 Size: 53 83 186 936 2560 382 635 1451 Fragments arranged by size: 2560 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4719 5117 5391 5538 5949 4719 Size: 398 274 147 411 5056 Fragments arranged by size: 5056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3219 3249 1178 Size: 30 165 30 1816 30 4215 Fragments arranged by size: 4215 1816 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6286 BseYI C'CCAG_C Cuts at: 5489 5985 5489 Size: 496 5790 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5713 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3736 4497 5525 1540 Size: 275 186 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 2179 2894 4347 4432 5503 2133 Size: 46 715 1453 85 1071 2916 Fragments arranged by size: 2916 1453 1071 715 85 46 BsmI GAATG_Cn' Cuts at: 3325 3797 3890 3325 Size: 472 93 5721 Fragments arranged by size: 5721 472 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2179 2235 2894 3605 4347 4432 Size: 399 343 46 56 659 711 742 85 Cuts at: 4432 5503 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 711 659 399 343 85 56 46 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 4175 5905 4175 Size: 1730 4556 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2857 3641 844 Size: 2013 784 3489 Fragments arranged by size: 3489 2013 784 BsrBI CCG'CTC Cuts at: 1394 1823 4173 1394 Size: 429 2350 3507 Fragments arranged by size: 3507 2350 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3204 4781 4955 65 Size: 1183 1956 1577 174 1396 Fragments arranged by size: 1956 1577 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4930 5117 1700 Size: 110 3120 187 2869 Fragments arranged by size: 3120 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 4346 5358 4346 Size: 1012 5274 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6286 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6286 BstYI r'GATC_y Cuts at: 1972 2376 3135 3616 3983 4368 4385 5837 5923 Size: 404 759 481 367 385 17 1452 86 Cuts at: 5923 5935 6281 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 759 481 404 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2865 2865 Size: 6286 BtgI C'CryG_G Cuts at: 513 1065 3154 513 Size: 552 2089 3645 Fragments arranged by size: 3645 2089 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5547 Fragments arranged by size: 5547 683 56 BtsI GCAGTG_nn' Cuts at: 2839 3802 4596 4616 2839 Size: 963 794 20 4509 Fragments arranged by size: 4509 963 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 2487 3590 2487 Size: 1103 5183 DraIII CAC_nnn'GTG Cuts at: 2365 2365 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2081 2337 3733 Size: 54 1425 50 275 18 251 256 1396 Cuts at: 3733 4622 6251 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1396 889 275 256 251 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4216 1353 Size: 2863 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4873 5247 5393 387 Size: 1064 138 3284 374 146 1280 Fragments arranged by size: 3284 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2131 2892 2131 Size: 761 5525 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2018 2511 4349 4946 5733 6001 1239 Size: 393 386 493 1838 597 787 268 1524 Fragments arranged by size: 1838 1524 787 597 493 393 386 268 EcoRI G'AATT_C Cuts at: 2496 3339 3595 3973 2496 Size: 843 256 378 4809 Fragments arranged by size: 4809 843 378 256 FspI TGC'GCA Cuts at: 4792 4792 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 3282 5433 1678 Size: 1604 2151 2531 Fragments arranged by size: 2531 2151 1604 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2311 2343 2600 2632 2919 Size: 32 135 32 739 32 257 32 287 Cuts at: 2919 2951 4927 4959 5001 5033 1373 Size: 32 1976 32 42 32 2626 Fragments arranged by size: 2626 1976 739 287 257 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3630 3876 669 Size: 988 281 1692 246 3079 Fragments arranged by size: 3079 1692 988 281 246 HindIII A'AGCT_T Cuts at: 748 2712 3105 748 Size: 1964 393 3929 Fragments arranged by size: 3929 1964 393 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KpnI G_GTAC'C Cuts at: 2564 3611 2564 Size: 1047 5239 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2809 5400 5584 1305 Size: 1504 2591 184 2007 Fragments arranged by size: 2591 2007 1504 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2083 2339 10 Size: 54 1425 343 251 256 3957 Fragments arranged by size: 3957 1425 343 256 251 54 MslI CAynn'nnrTG Cuts at: 518 1166 4244 4603 4762 518 Size: 648 3078 359 159 2042 Fragments arranged by size: 3078 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2407 3233 3702 4379 5527 5772 1606 Size: 801 826 469 677 1148 245 2120 Fragments arranged by size: 2120 1148 826 801 677 469 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5734 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 2671 3644 5189 1132 Size: 97 1442 973 1545 2229 Fragments arranged by size: 2229 1545 1442 973 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6286 PciI A'CATG_T Cuts at: 2667 5185 2667 Size: 2518 3768 PflMI CCAn_nnn'nTGG Cuts at: 1108 3469 1108 Size: 2361 3925 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4355 4387 5868 5900 4355 Size: 32 1481 32 4741 Fragments arranged by size: 4741 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6286 PsiI TTA'TAA Cuts at: 3312 3856 3312 Size: 544 5742 PstI C_TGCA'G Cuts at: 830 3638 830 Size: 2808 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 PvuII CAG'CTG Cuts at: 2407 3233 2407 Size: 826 5460 SacI G_AGCT'C Cuts at: 2133 2894 2133 Size: 761 5525 SalI G'TCGA_C Cuts at: 1936 3628 1936 Size: 1692 4594 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 4534 6246 4534 Size: 1712 4574 SexAI A'CCwGG_T Cuts at: 3449 3449 Size: 6286 SfcI C'TryA_G Cuts at: 826 1045 2657 2704 3634 4769 5450 5641 826 Size: 219 1612 47 930 1135 681 191 1471 Fragments arranged by size: 1612 1471 1135 930 681 219 191 47 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3613 1872 Size: 1741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2515 4390 5291 5553 820 Size: 197 926 572 1875 901 262 1553 Fragments arranged by size: 1875 1553 926 901 572 262 197 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 3644 1132 Size: 2512 3774 SspI AAT'ATT Cuts at: 5 52 4210 5 Size: 47 4158 2081 Fragments arranged by size: 4158 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6286 StyI C'CwwG_G Cuts at: 513 1065 3725 513 Size: 552 2660 3074 Fragments arranged by size: 3074 2660 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4313 4330 4483 4668 5113 325 Size: 1255 2733 17 153 185 445 1498 Fragments arranged by size: 2733 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2508 4532 6244 Size: 274 80 33 51 1443 531 2024 1712 Cuts at: 6244 96 Size: 138 Fragments arranged by size: 2024 1712 1443 531 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3219 3251 6246 6278 3219 Size: 32 2995 32 3227 Fragments arranged by size: 3227 2995 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6286 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6286 XmaI C'CCGG_G Cuts at: 1870 3611 1870 Size: 1741 4545 XmnI GAAnn'nnTTC Cuts at: 4415 4415 Size: 6286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI