(Linear) (Six-base) MAPSORT of: KIEE6450.seq Check: 5719 from: 1 to: 1581 KIEE6450.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. Acc65I G'GTAC_C Cuts at: 0 555 1581 Size: 555 1026 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 1581 Size: 13 1568 AfeI AGC'GCT Cuts at: 0 1275 1581 Size: 1275 306 AflIII A'CryG_T Cuts at: 0 662 1581 Size: 662 919 AhdI GACnn_n'nnGTC Cuts at: 0 609 1581 Size: 609 972 ApaLI G'TGCA_C Cuts at: 0 170 1581 Size: 170 1411 ApoI r'AATT_y Cuts at: 0 491 633 755 787 874 1334 1546 1581 Size: 491 142 122 32 87 460 212 35 Fragments arranged by size: 491 460 212 142 122 87 35 32 AseI AT'TA_AT Cuts at: 0 1292 1581 Size: 1292 289 BanI G'GyrC_C Cuts at: 0 225 555 1581 Size: 225 330 1026 Fragments arranged by size: 1026 330 225 BanII G_rGCy'C Cuts at: 0 128 889 1581 Size: 128 761 692 Fragments arranged by size: 761 692 128 BbsI GAAGACnn'nnnn_ Cuts at: 0 723 1368 1581 Size: 723 645 213 Fragments arranged by size: 723 645 213 BbvCI CC'TCA_GC Cuts at: 0 969 1581 Size: 969 612 BciVI GTATCCnnnnn_n' Cuts at: 0 1055 1581 Size: 1055 526 BclI T'GATC_A Cuts at: 0 1185 1344 1581 Size: 1185 159 237 Fragments arranged by size: 1185 237 159 BglII A'GATC_T Cuts at: 0 371 1581 Size: 371 1210 BlpI GC'TnA_GC Cuts at: 0 1270 1581 Size: 1270 311 Bme1580I G_kGCm'C Cuts at: 0 174 230 1581 Size: 174 56 1351 Fragments arranged by size: 1351 174 56 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 536 1581 Size: 536 1045 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 506 1581 Size: 506 1075 Bpu10I CC'TnA_GC Cuts at: 0 888 969 1581 Size: 888 81 612 Fragments arranged by size: 888 612 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 531 1581 Size: 531 1050 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1214 1244 1581 Size: 1214 30 337 Fragments arranged by size: 1214 337 30 BsiHKAI G_wGCw'C Cuts at: 0 128 174 889 1581 Size: 128 46 715 692 Fragments arranged by size: 715 692 128 46 BsmI GAATG_Cn' Cuts at: 0 1320 1581 Size: 1320 261 Bsp1286I G_dGCh'C Cuts at: 0 128 174 230 889 1581 Size: 128 46 56 659 692 Fragments arranged by size: 692 659 128 56 46 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 852 1581 Size: 852 729 BsrDI GCAATG_nn' Cuts at: 0 1199 1581 Size: 1199 382 BstYI r'GATC_y Cuts at: 0 371 1130 1581 Size: 371 759 451 Fragments arranged by size: 759 451 371 Bsu36I CC'TnA_GG Cuts at: 0 860 1581 Size: 860 721 BtgI C'CryG_G Cuts at: 0 1149 1581 Size: 1149 432 BtsI GCAGTG_nn' Cuts at: 0 834 1581 Size: 834 747 DraI TTT'AAA Cuts at: 0 482 1581 Size: 482 1099 DraIII CAC_nnn'GTG Cuts at: 0 360 1581 Size: 360 1221 EaeI y'GGCC_r Cuts at: 0 76 332 1581 Size: 76 256 1249 Fragments arranged by size: 1249 256 76 EcoICRI GAG'CTC Cuts at: 0 126 887 1581 Size: 126 761 694 Fragments arranged by size: 761 694 126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 506 1581 Size: 13 493 1075 Fragments arranged by size: 1075 493 13 EcoRI G'AATT_C Cuts at: 0 491 1334 1581 Size: 491 843 247 Fragments arranged by size: 843 491 247 HaeII r_GCGC'y Cuts at: 0 1277 1581 Size: 1277 304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 306 338 595 627 914 946 1581 Size: 306 32 257 32 287 32 635 Fragments arranged by size: 635 306 287 257 32 32 32 HindIII A'AGCT_T Cuts at: 0 707 1100 1581 Size: 707 393 481 Fragments arranged by size: 707 481 393 KpnI G_GTAC'C Cuts at: 0 559 1581 Size: 559 1022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 804 1581 Size: 804 777 MscI TGG'CCA Cuts at: 0 78 334 1581 Size: 78 256 1247 Fragments arranged by size: 1247 256 78 MspA1I CmG'CkG Cuts at: 0 402 1228 1581 Size: 402 826 353 Fragments arranged by size: 826 402 353 NspI r_CATG'y Cuts at: 0 666 1581 Size: 666 915 PciI A'CATG_T Cuts at: 0 662 1581 Size: 662 919 PflMI CCAn_nnn'nTGG Cuts at: 0 1464 1581 Size: 1464 117 PsiI TTA'TAA Cuts at: 0 1307 1581 Size: 1307 274 PvuII CAG'CTG Cuts at: 0 402 1228 1581 Size: 402 826 353 Fragments arranged by size: 826 402 353 SacI G_AGCT'C Cuts at: 0 128 889 1581 Size: 128 761 692 Fragments arranged by size: 761 692 128 SexAI A'CCwGG_T Cuts at: 0 1444 1581 Size: 1444 137 SfcI C'TryA_G Cuts at: 0 652 699 1581 Size: 652 47 882 Fragments arranged by size: 882 652 47 SmlI C'TyrA_G Cuts at: 0 510 1581 Size: 510 1071 TatI w'GTAC_w Cuts at: 0 503 1581 Size: 503 1078 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1214 1246 1581 Size: 1214 32 335 Fragments arranged by size: 1214 335 32 Enzymes that do cut: Acc65I AcuI AfeI AflIII AhdI ApaLI ApoI AseI BanI BanII BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaXI BsiHKAI BsmI Bsp1286I BspMI BsrDI BstYI Bsu36I BtgI BtsI DraI DraIII EaeI EcoICRI Eco57MI EcoRI HaeII Hin4I HindIII KpnI MmeI MscI MspA1I NspI PciI PflMI PsiI PvuII SacI SexAI SfcI SmlI TatI TstI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI AleI AlfI AloI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BfrBI BglI BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DrdI EagI EarI EciI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HincII HpaI KasI MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI